Comparative pathogenicity of two closely related Newcastle disease virus isolates from chicken and pigeon respectively

Zhan, Tiansong; Hu, Shunlin; Chen, Yu; Lü, Xiaolong; Liao, Tianxing; Guo, Lili; Gao, Xiaomin; Xu, Xiaoting; Hu, Zenglei; Wang, Xiaoquan

doi:10.1016/j.virusres.2020.198091

Cited by 10 publications

(18 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This shall avoid mischaracterization of the pathogenic nature (velogenic/mesogenic/lentogenic) of the isolated strains, especially with increased detection of lentogenic strains that possess a velogenic motif at their F protein cleavage site. (2) For NDV isolates from birds rather than chicken, pathogenicity indices shall be performed in both actual bird of origin and chicken, as different indices may implicate the adaptation of the virus in both hosts, despite the close genetic relatedness (179)…”

Section: Discussionmentioning

confidence: 99%

Avian Paramyxovirus Type 1 in Egypt: Epidemiology, Evolutionary Perspective, and Vaccine Approach

et al. 2021

View full text Add to dashboard Cite

Avian orthoavulavirus 1, formerly known as avian paramyxovirus type-1 (APMV-1), infects more than 250 different species of birds. It causes a broad range of clinical diseases and results in devastating economic impact due to high morbidity and mortality in addition to trade restrictions. The ease of spread has allowed the virus to disseminate worldwide with subjective virulence, which depends on the virus strain and host species. The emergence of new virulent genotypes among global epizootics, including those from Egypt, illustrates the time-to-time genomic alterations that lead to simultaneous evolution of distinct APMV-1 genotypes at different geographic locations across the world. In Egypt, the Newcastle disease was firstly reported in 1947 and continued to occur, despite rigorous prophylactic vaccination, and remained a potential threat to commercial and backyard poultry production. Since 2005, many researchers have investigated the nature of APMV-1 in different outbreaks, as they found several APMV-1 genotypes circulating among various species. The unique intermingling of migratory, free-living, and domesticated birds besides the availability of frequently mobile wild birds in Egypt may facilitate the evolution power of APMV-1 in Egypt. Pigeons and waterfowls are of interest due to their inclusion in Egyptian poultry industry and their ability to spread the infection to other birds either by presence of different genotypes (as in pigeons) or by harboring a clinically silent disease (as in waterfowl). This review details (i) the genetic and pathobiologic features of APMV-1 infections in Egypt, (ii) the epidemiologic and evolutionary events in different avian species, and (iii) the vaccine applications and challenges in Egypt.

show abstract

Section: Discussionmentioning

confidence: 99%

Avian Paramyxovirus Type 1 in Egypt: Epidemiology, Evolutionary Perspective, and Vaccine Approach

et al. 2021

View full text Add to dashboard Cite

show abstract

“…NDV strains LX, La Sota, Mukterswar, Herts/33, NDV-P05, Kuwait, JS 5/05, and ZJ1 were isolated and identified by our laboratory. The biological characteristics of these NDV isolates were determined in previous studies [28][29][30][31][32] and are shown in Table 1.…”

Section: Cell Lines and Virus Propagationmentioning

confidence: 99%

“…The prediction results of different strains were intersected using Venny 2.1 (https://bioinfogp.cnb.csic.es/tools/venny/) and the overlaps were selected for conservation analysis. We aligned predicted miRNA binding sites in 192 NDV strains collected from GenBank using MEGA 5 software (DNAStar Inc. USA) and genotyping of these strains was performed using an updated classification system as described in our previous study [29].…”

Section: Potential Mirnas Prediction and Conservation Analysismentioning

confidence: 99%

gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

Chen

Zhu

et al. 2020

Virulence

Self Cite

View full text Add to dashboard Cite

“…With the rapid development and availability of molecular methods, these are now almost universally utilized for characterization of avian paramyxovirus isolates. Although many pigeon-derived viruses have been found to possess multiple basic amino acid residues at the F-protein-cleavage site, which is typical for virulent NDV, experimental inoculation of many of these viruses revealed variable clinical outcomes in chickens [ 22 , 23 , 24 , 25 , 26 ]. While PPMV-1 commonly produce high morbidity and mortality in pigeons, infections in chickens often present with mild to moderate neurological signs [ 23 , 24 , 27 ] or even absence of clinical disease [ 22 , 25 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…Although many pigeon-derived viruses have been found to possess multiple basic amino acid residues at the F-protein-cleavage site, which is typical for virulent NDV, experimental inoculation of many of these viruses revealed variable clinical outcomes in chickens [ 22 , 23 , 24 , 25 , 26 ]. While PPMV-1 commonly produce high morbidity and mortality in pigeons, infections in chickens often present with mild to moderate neurological signs [ 23 , 24 , 27 ] or even absence of clinical disease [ 22 , 25 , 28 ]. These findings are also supported by establishing the intracerebral pathogenicity index (ICPI), with many pigeon-derived viruses having values below 1.5 [ 28 , 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

A Pigeon-Derived Sub-Genotype XXI.1.2 Newcastle Disease Virus from Bangladesh Induces High Mortality in Chickens

Nooruzzaman

Barman

Mumu

et al. 2021

Viruses

View full text Add to dashboard Cite

Newcastle disease virus (NDV) is a significant pathogen of poultry; however, variants also affect other species, including pigeons. While NDV is endemic in Bangladesh, and poultry isolates have been recently characterized, information about viruses infecting pigeons is limited. Worldwide, pigeon-derived isolates are commonly of low to moderate virulence for chickens. Here, we studied a pigeon-derived NDV isolated in Bangladesh in 2010. To molecularly characterize the isolate, we sequenced its complete fusion gene and performed a comprehensive phylogenetic analysis. We further studied the biological properties of the virus by estimating mean death time (MDT) and by experimentally infecting 5-week-old naïve Sonali chickens. The studied virus clustered in sub-genotype XXI.1.2 with NDV from pigeons from Pakistan isolated during 2014–2018. Deduced amino acid sequence analysis showed a polybasic fusion protein cleavage site motif, typical for virulent NDV. The performed in vivo pathogenicity testing showed a MDT of 40.8 h, and along with previously established intracerebral pathogenicity index of 1.51, these indicated a velogenic pathotype for chickens, which is not typical for pigeon-derived viruses. The experimental infection of chickens resulted in marked neurological signs and high mortality starting at 7 days post infection (dpi). Mild congestion in the thymus and necrosis in the spleen were observed at an advanced stage of infection. Microscopically, lymphoid depletion in the thymus, spleen, and bursa of Fabricius were found at 5 dpi, which progressed to severe in the following days. Mild to moderate proliferation of glial cells was noticed in the brain starting at 2 dpi, which gradually progressed with time, leading to focal nodular aggregation. This study reports the velogenic nature for domestic chickens of a pigeon-derived NDV isolate of sub-genotype XXI.1.2. Our findings show that not all pigeon-derived viruses are of low virulence for chickens and highlight the importance of biologically evaluating the pathogenicity of NDV isolated from pigeons.

show abstract

Comparative pathogenicity of two closely related Newcastle disease virus isolates from chicken and pigeon respectively

Cited by 10 publications

References 69 publications

Avian Paramyxovirus Type 1 in Egypt: Epidemiology, Evolutionary Perspective, and Vaccine Approach

Avian Paramyxovirus Type 1 in Egypt: Epidemiology, Evolutionary Perspective, and Vaccine Approach

gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

A Pigeon-Derived Sub-Genotype XXI.1.2 Newcastle Disease Virus from Bangladesh Induces High Mortality in Chickens

Contact Info

Product

Resources

About