gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

Chen, Yu; Zhu, Shanshan; Hu, Jiao; Hu, Zenglei; Liu, Xiaowen; Wang, Xiaoquan; Gu, Minghong; Hu, Shunlin

doi:10.1080/21505594.2020.1864136

Cited by 7 publications

(5 citation statements)

References 49 publications

(47 reference statements)

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“…The virus replicates constantly in cells. When the virus was inoculated at an MOI of 1, the virus replication kinetics did not reach a plateau at 30 hpi ( 44 , 45 ). When the mRNA expression of NOD1 was suppressed, the DHAV-3 genomic copy number dropped significantly.…”

Section: Discussionmentioning

confidence: 99%

NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

et al. 2021

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Duck viral hepatitis (DVH) is an acute, highly lethal infectious disease of ducklings that causes huge losses in the duck industry. Duck hepatitis A virus genotype 3 (DHAV-3) has been one of the most prevalent DVH pathogen in the Asian duck industry in recent years. Here, we investigated the genetic basis of the resistance and susceptibility of ducks to DVH by comparing the genomes and transcriptomes of a resistant Pekin duck flock (Z8) and a susceptible Pekin duck flock (SZ7). Our comparative genomic and transcriptomic analyses suggested that NOD1 showed a strong signal of association with DVH susceptibility in ducks. Then, we found that NOD1 showed a significant expression difference between the livers of susceptible and resistant individuals after infection with DHAV-3, with higher expression in the SZ7 flock. Furthermore, suppression and overexpression experiments showed that the number of DHAV-3 genomic copies in primary duck hepatocytes was influenced by the expression level of NOD1. In addition, in situ RNAscope analysis showed that the localization of NOD1 and DHAV-3 in liver cells was consistent. Altogether, our data suggested that NOD1 was likely associated with DHAV-3 susceptibility in ducks, which provides a target for future investigations of the pathogenesis of DVH.

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Section: Discussionmentioning

confidence: 99%

NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

et al. 2021

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“…The microRNAs (miRNAs) have been shown to regulate viral replication by interacting with various host mRNAs [ 130 ]. Based on this, Chen et al [ 131 ] further investigated the interaction between miRNAs and NDV genes associated with viral replication. They revealed that miRNAs could regulate the infection of NDV through interacting with the L gene.…”

Section: Main Textmentioning

confidence: 99%

The multifaceted interactions between Newcastle disease virus proteins and host proteins: a systematic review

Lu,

Wang,

Liu

et al. 2024

Virulence

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Newcastle disease virus (NDV) typically induces severe illness in poultry and results in significant economic losses for the worldwide poultry sector. NDV, an RNA virus with a single-stranded negative-sense genome, is susceptible to mutation and immune evasion during viral transmission, thus imposing enormous challenges to avian health and poultry production. NDV is composed of six structural proteins and two nonstructural proteins that exert pivotal roles in viral infection and antiviral responses by interacting with host proteins. Nowadays, there is a particular focus on the mechanisms of virus-host protein interactions in NDV research, yet a comprehensive overview of such research is still lacking. Herein, we briefly summarize the mechanisms regarding the effects of virus-host protein interaction on viral infection, pathogenesis, and host immune responses. This review can not only enhance the present comprehension of the mechanism underlying NDV and host interplay, but also furnish a point of reference for the advancement of antiviral measures.

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“…These changes in miRNA expression profiles could be a strategy used by RNA viruses when escaping from the host cell immune system through the fine-tuning of miRNA on intracellular protein expression, or it could be an immune reaction, meaning that the body perceives the invasion of RNA viruses and fine-tunes immune-related genes through the miRNA system to coordinate the whole immune response. Since miRNA can bind to mRNA and regulate its translation, it is reasonable to suggest that the positive RNA from RNA viruses, which carry translatable genetic information, could be targeted and regulated by the host miRNAs ( Zheng et al., 2018 ; Li et al., 2019 ; Chen et al., 2021 ). Up until now, there have been numerous experiments conducted to try and confirm this hypothesis.…”

Section: Interactions Between Mirnas and Virusesmentioning

confidence: 99%

“…Similarly, the virulent newcastle disease virus (NDV) is a negative-strand RNA virus and is translated into viral protein after NDV has been transcribed for a positive-sense RNA upon entry into host cells. gga-miR-1603 and gga-miR-1794 (gga, chicken) were confirmed to target two highly conserved regions of the L gene of NDV, to inhibit the expression of the L protein at both the protein and RNA levels, thus suppressing NDV replication ( Chen et al., 2021 ). Both of these examples are based on the RNA-silencing effects of miRNA in inhibiting protein translation within RNA viruses, and some more interesting examples of miRNA and RNA viruses are mentioned below.…”

Section: Mirnas Regulate Gene Expressionmentioning

confidence: 99%

The Influence of Host miRNA Binding to RNA Within RNA Viruses on Virus Multiplication

Liu

Cheng

Wang

et al. 2022

Front. Cell. Infect. Microbiol.

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microRNAs (miRNAs), non-coding RNAs about 22 nt long, regulate the post-transcription expression of genes to influence many cellular processes. The expression of host miRNAs is affected by virus invasion, which also affects virus replication. Increasing evidence has demonstrated that miRNA influences RNA virus multiplication by binding directly to the RNA virus genome. Here, the knowledge relating to miRNAs’ relationships between host miRNAs and RNA viruses are discussed.

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gga-miR-1603 and gga-miR-1794 directly target viral L gene and function as a broad-spectrum antiviral factor against NDV replication

Cited by 7 publications

References 49 publications

NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

NOD1 Is Associated With the Susceptibility of Pekin Duck Flock to Duck Hepatitis A Virus Genotype 3

The multifaceted interactions between Newcastle disease virus proteins and host proteins: a systematic review

The Influence of Host miRNA Binding to RNA Within RNA Viruses on Virus Multiplication

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