Comparative pathogenicity of Vibrio spp., Photobacterium damselae ssp. damselae and five isolates of Aeromonas salmonicida ssp. achromogenes in juvenile Atlantic halibut (Hippoglossus hippoglossus)

Abstract

Juvenile Atlantic halibut (~100 mg, Hippoglossus hippoglossus) were exposed to Vibrio proteolyticus, a Vibrio spp. isolate, Photobacterium damselae ssp. damselae and five different isolates of Aeromonas salmonicida ssp. achromogenes via an hour‐long bath immersion to ascertain their variation in pathogenicity to this fish species. Results were analysed using Kaplan–Meier survival analysis. Analysis of the data from challenges using A. salmonicida ssp. achromogenes revealed three survival values of zero and a spread of values from 0 to 28.43. Challenges using a Vibrio spp isolate, V. proteolyticus and P. damselae resulted in Kaplan–Meier survival estimates of 31.21, 50.41 and 57.21, respectively. As all bacterial species tested could induce juvenile halibut mortalities, they must all be considered as potential pathogens. However, the degree of pathogenicity of A. salmonicida is isolate dependent.

A Vibrio T6SS-Mediated Lethality in an Aquatic Animal Model

“… Vibrio proteolyticus ( Vpr ) is a Gram-negative marine bacterium. Vpr strains were isolated from diseased corals ( 3 ) and were shown to be virulent to fish ( 4 ) and crustaceans ( 5 ). We previously reported that Vpr strain NBRC 13287 (ATCC 15338) has 3 anti-eukaryotic determinants: a secreted pore-forming hemolysin (VPRH) that kills mammalian cells, and 2 type VI secretion systems (T6SS1 and T6SS3) that deliver anti-eukaryotic effector proteins into mammalian phagocytic cells ( 6 – 8 ).…”

Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

“…In addition, despite being the most reliable method, the challenge method by intraperitoneal injection is not the natural route of infection and thus might affect the results (Bowden et al. ).…”

Post-phagocytosis activation of NLRP3 inflammasome by two novel T6SS effectors

“…The copyright holder for this preprint this version posted August 12, 2022. ; https://doi.org/10. 1101/2022 Vibrio proteolyticus (V. proteolyticus) is a Gram-negative marine bacterium that was isolated from diseased corals (Cervino et al, 2008); it was also shown to cause mortality in fish (Bowden et al, 2018) and in the crustacean model organism, Artemia (Verschuere et al, 2000). V. proteolyticus does not possess a type III secretion system (T3SS) that can mediate delivery of effector proteins directly into host cells (Miller et al, 2019;Ray et al, 2016).…”