Comparative molecular prevalence and subtypes distribution of Blastocystis sp. a potentially zoonotic infection isolated from symptomatic and asymptomatic patients in Iran: A systematic review and meta-analysis

Asghari, Ali; Hassanipour, Soheil; Hatam, Gholamreza

doi:10.1007/s11686-021-00360-0

Cited by 23 publications

(13 citation statements)

References 55 publications

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“…Blastocystis sp. is an intestinal parasite whose pathogenic role is underestimated; several studies reported its presence in both asymptomatic and symptomatic patients [26,27]. In Syria, epidemiological and molecular studies on this parasite are scarce.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria

2023

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Blastocystis sp. is an enteric protist found in humans and a wide range of animal hosts. Genetic variations were established among the 38 different subtypes detected so far, 14 of which are commonly found in human and animal hosts. The aim of the present study is to estimate the prevalence of the common Blastocystis subtypes and evaluate the possible correlation with several variables (gender, age, symptoms, domestic animals…), among patients from the southern region of Syria. Fecal samples were collected from individuals suffering from gastrointestinal complaints. Microscopic examination along with genotype analyses using seven pairs of subtype-specific primers was performed. Our results revealed the presence of Blastocystis sp. in 46 isolates out of the 60 samples microscopically studied (76.7%); single infection was detected in 24 isolates whereas co-infection with other protozoa was identified in 22 ones. Molecular detection targeting the SSU rRNA gene revealed a 100% positive presence of Blastocystis sp. in all the samples. Genotyping results detected the presence of five different subtypes (ST1-ST5) with varying proportions. However, ST1 was the dominant subtype observed (66.7%). Mixed subtype infections were found in 9 isolates (15%). Three samples remained undefined, nonetheless. Our statistical results showed no significant correlation between Blastocystis STs infection and the different studied variables. In conclusion, this study provides the first genetic characterization of Blastocystis subtypes prevalence in patients from the southern region of Syria. ST1 distribution was highly predominant. Further molecular studies are needed to estimate the prevalence of Blastocystis sp. infection in other regions in Syria and to understand the epidemiology and sources of transmission to humans.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria

2023

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“…Blastocystis hominis was the most common detected parasite followed by Entamoeba coli, Giardia lamblia, Cryptosporidium, Iodamoeba butchlii, and Endolimax nana. Blastocystis is considered a common protozoan infection in humans in Iran [11][12][13][14][15][16]. Results of a recent systematic review and meta-analysis revealed that Entamoeba coli, Blastocystis spp., and Giardia lamblia are the most prevalent protozoan parasite among intellectually disabled individuals in Iran [11].…”

Section: Discussionmentioning

confidence: 99%

Intestinal Parasitic Infections among Intellectually Disabled Individuals in Bandar Abbas County, Southern Iran

Pakmehr

Omidian

Turki

et al. 2022

Journal of Parasitology Research

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Intellectually disabled individuals are more prone to parasitic infections due to their unusual behaviors, immune and nutrient deficiencies, and living conditions. The current study is aimed at evaluating the prevalence of intestinal parasites in institutionalized intellectually disabled individuals in Bandar Abbas County in the south of Iran. Subjects of the study were 119 individuals, living in an intellectually disabled individual care center. Demographic features of the subjects including age, sex, intellectual disability type, and duration of their stay in the center were recorded. A stool sample was taken from each subject and evaluated by direct wet mount and formalin-ethyl-acetate concentration methods. Samples were also examined, using trichrome and modified acid-fast permanent staining. The mean age of the subjects was 27.6 (±2.24), ranging from 4 to 60 years old. Of the 119 participants, 55 (46.2%) were male, and 64 cases (53.8%) were female. Overall, 31 individuals (26.1%, 95% CI: 18.4-34.9) were found to be infected with at least one type of intestinal parasite. Blastocystis hominis, as the most common detected parasite, was detected in 13 (10.1%), Entamoeba coli in 12 (10.1%), Giardia lamblia in 5 (4.2%), Cryptosporidium in 2 (1.7%), Iodamoeba butchlii in 2 (1.7%), and Endolimax nana in 1 (0.8%) of participants. Three cases had coinfection with Blastocystis hominis and Entamoeba coli, one case was infected with Blastocystis hominis and Giardia lamblia, and one case was coinfected with Giardia lamblia and Entamoeba coli. There were no statistically significant associations between intestinal parasitic infection and gender, age, type of intellectual disability, or duration of stay in the care center ( P > 0.05 ). The findings of the present study indicate a relatively high prevalence of parasitic infections in people with intellectual disabilities in Bandar Abbas, southern Iran. Noteworthy is the high prevalence of Blastocyst and also the presence of Cryptosporidium infection in these people. Periodic treatment of these people and improvement of their maintenance conditions can be considered for the prevention and control of intestinal parasitic infection in these people.

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“…Meta-analysis was done using Comprehensive Meta-analysis (CMA) v3 software, with p-values <0.05 as statistically significant [ 17 , 18 ]. Cochrane's Q and I 2 statistics (with a significance level of 50%) were employed to evaluate the potential heterogeneity in studies.…”

Section: Methodsmentioning

confidence: 99%

Global molecular prevalence of Giardia duodenalis in pigs (Sus domesticus): A systematic review and meta-analysis

Asghari¹,

Ebrahimi²,

Shamsi³

et al. 2023

Heliyon

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Comparative molecular prevalence and subtypes distribution of Blastocystis sp. a potentially zoonotic infection isolated from symptomatic and asymptomatic patients in Iran: A systematic review and meta-analysis

Cited by 23 publications

References 55 publications

Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria

Molecular characterization of Blastocystis subtypes in symptomatic patients from the southern region of Syria

Intestinal Parasitic Infections among Intellectually Disabled Individuals in Bandar Abbas County, Southern Iran

Global molecular prevalence of Giardia duodenalis in pigs (Sus domesticus): A systematic review and meta-analysis

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