Comparative metabolomics of Himalayan crab apple (Malus baccata) with commercially utilized apple (Malus domestica) using UHPLC-QTOF-IMS coupled with multivariate analysis

Dadwal, V. S.; Joshi, Robin; Gupta, Mahesh

doi:10.1016/j.foodchem.2022.134529

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…Data mining is a popular method for evaluating interactions between different datasets, particularly in phytochemical studies where it can help identify the compound(s) responsible for reported biological activities [62, 63]. In our study, we performed multivariate analysis to determine interactions between the tested biological activities as well as the extraction methods.…”

Section: Resultsmentioning

confidence: 99%

Extractions of aerial parts of Hippomarathrum scabrum with conventional and green methodologies: Chemical profiling, antioxidant, enzyme inhibition, and anti‐cancer effects

Nilofar,

Duran,

Uba

et al. 2023

J of Separation Science

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Hippomarathrum scabrum L. is an endemic medicinal plant in Turkey; however, there have been few studies investigating the phytochemistry and biological properties of these plants has not been investigated. The aim of this work is to determine the chemical composition of different extracts (extracts obtained by using supercritical carbon dioxide extraction, accelerated solvent extraction, homogenizer‐assisted extraction, microwave‐assisted extraction, and ultrasound‐assisted extraction from Hippomarathrum scabrum L., and evaluate their biological properties. The analysis revealed that 5‐O‐caffeoylquinic acid, rutin, and isorhamnetin 3‐O‐rutinoside were the main bioactive compounds. The extract obtained by accelerated extraction contains the highest concentration of 5‐O‐Caffeoylquinic acid (7616.74 ± 63.09 mg/kg dry extract) followed by the extract obtained by homogenizer‐assisted extraction (6682.53 ± 13.04 mg/kg dry extract). In antioxidant tests, all extracts expressed significant antioxidant activity. Also, cytotoxic and anticancer effects of these plant extracts were detected in the human prostate cancer cell line. Intrinsic apoptotic genes were up‐regulated and anti‐apoptotic genes were down‐regulated in human prostate cancer cells after inhibition concentration dose treatment. The findings are promising, and suggest the use of these plant extracts could be used as natural sources with different biological activities, as well as anticancer agents.

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Section: Resultsmentioning

confidence: 99%

Extractions of aerial parts of Hippomarathrum scabrum with conventional and green methodologies: Chemical profiling, antioxidant, enzyme inhibition, and anti‐cancer effects

Nilofar,

Duran,

Uba

et al. 2023

J of Separation Science

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“…In the case of Nasturtium, five flavonoid glycosides, five phenolic acids, one flavanone, one flavone, and one phenolic aldehyde were identified (Table 3, Figure S2). Five phenolic acids (peaks 12, 13, 18 tively. Among flavonoid glycosides (peaks 17, 20, 22, 23, and 24), quercetin pentoside, cyanidin hexosyl deoxyhexoside, delphinidin dihexoside, HHDP-galloyl hexoside, and cyanidin-(malonylhexosyl)-hexoside were identified (Table 3).…”

Section: Uhplc-qtof-ims-based Quantification Of Phenolics and Flavono...mentioning

confidence: 99%

“…13,17 Metabolomics is an evolving and widely deployed omics tool for quantifying and analyzing the distribution of different metabolites. 18 In the present study, we evaluated the phytochemical composition of selected edible flowers using both targeted and non-targeted metabolomics approaches and identified several metabolites that could find use as marker compounds and understood the distribution of a different class of metabolites that were earlier not identified or reported. Further, the determination of the bioaccessibility of phytochemicals from edible flowers is important, as the gastrointestinal environment can change the structures and biological characteristics of polyphenols, thus affecting their bioactivity.…”

Section: Introductionmentioning

confidence: 99%

“…Metabolomics is an evolving and widely deployed omics tool for quantifying and analyzing the distribution of different metabolites . In the present study, we evaluated the phytochemical composition of selected edible flowers using both targeted and non-targeted metabolomics approaches and identified several metabolites that could find use as marker compounds and understood the distribution of a different class of metabolites that were earlier not identified or reported.…”

Section: Introductionmentioning

confidence: 99%

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Wild Edible Flowers of Western Himalayas: Nutritional Characterization, UHPLC-QTOF-IMS-Based Phytochemical Profiling, Antioxidant Properties, and In Vitro Bioaccessibility of Polyphenols

Hegde,

Gupta,

Kumari

et al. 2023

ACS Omega

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Four edible flowers commonly consumed in the Western Himalayan region, namely, Bauhinia variegata (Kachnar), Tropaeolum majus (Nasturtium), Matricaria chamomilla (Chamomile), and Tagetes erecta (Marigold), were characterized for their nutritional and phytochemical composition. Through the UHPLC-QTOF-IMS-based metabolomics approach, 131 compounds were tentatively identified consisting of phenolic acids, flavonoid glycosides, terpenoids, amino acids, and fatty acid derivatives. Kaempferol and quercetin glycosides for Kachnar, apigenin glycosides and caffeoylquinic acid derivatives for Chamomile, patulin and quercetin derivatives for Marigold, cyanidin and delphinidin glycosides for Nasturtium were the predicted marker metabolites identified through non-targeted metabolomics. Kachnar and Chamomile scored best in terms of macronutrients and essential micronutrients, respectively. Nasturtium contained high concentrations of α-linolenic acid, anthocyanins, and lutein. Kachnar contained the highest total phenolic acids (63.36 ± 0.38 mg GAE g–1), while Marigold contained the highest total flavonoids (118.90 ± 1.30 mg QUE g–1). Marigolds possessed excellent free radical scavenging and metal chelation activities. Chamomile exhibited strong α-glucosidase inhibition activity, followed by Nasturtium. The in vitro gastrointestinal digestibility of flower extracts indicated that the bioaccessibility of phenolic acids was higher than that of flavonoids. Polyphenols from Nasturtium and Chamomile showed the highest bioaccessibility. The study is an attempt to characterize traditionally consumed edible flowers and promote their wider utilization in gastronomy and nutraceuticals.

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“…Conversely, the integration of web-based computing tools and food science has introduced innovative methodologies in flavor research that facilitate the exploration of metabolite structural characteristics and enable early detection of potential key flavor indicators in conjunction with omics technologies. Non-targeted metabolomics techniques, particularly ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry (UHPLC-Q-TOF-MS), have recently gained significant popularity for identifying essential food metabolites [6][7][8][9] . Metabolomics studies typically rely on in-house or public databases for metabolite annotation.…”

Section: Introductionmentioning

confidence: 99%

Discovery of taste-active metabolites in Lactobacillus plantarum-fermented chili sauce via web-based computational analysis

Wang,

Mei,

Jin

et al. 2024

Food Science and Human Wellness

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The utilization of Lactobacillus plantarum (LP) in chili sauce production is well-known for its capacity to enhance product quality and sensory attributes. However, there is still limited knowledge regarding the taste-active metabolites in the sauce. To bridge this gap, our study employed metabolomics and web-based computational tools to investigate the dynamic changes of taste-active metabolites during chili sauce fermentation. By leveraging the advantages of the feature-based molecular network (FBMN), we conducted a rapid annotation of metabolites, successfully identifying 205 metabolites, a considerable portion of which were previously unreported. Through the utilization of the VirtualTaste tool, we identified dihydrosphingosine, lactic acid, isoleucine, phytosphingosine, and gluconic acid as potential taste indicators for quality control. Pathway enrichment analysis further supported their primary involvement in key biochemical pathways, including amino acid tRNA biosynthesis, phenylalanine, tyrosine, tryptophan biosynthesis, and sphingolipid metabolism. This investigation provides valuable insights into the underlying mechanisms contributing to the distinctive flavor profile of chili sauce.

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Comparative metabolomics of Himalayan crab apple (Malus baccata) with commercially utilized apple (Malus domestica) using UHPLC-QTOF-IMS coupled with multivariate analysis

Cited by 7 publications

References 37 publications

Extractions of aerial parts of Hippomarathrum scabrum with conventional and green methodologies: Chemical profiling, antioxidant, enzyme inhibition, and anti‐cancer effects

Extractions of aerial parts of Hippomarathrum scabrum with conventional and green methodologies: Chemical profiling, antioxidant, enzyme inhibition, and anti‐cancer effects

Wild Edible Flowers of Western Himalayas: Nutritional Characterization, UHPLC-QTOF-IMS-Based Phytochemical Profiling, Antioxidant Properties, and In Vitro Bioaccessibility of Polyphenols

Discovery of taste-active metabolites in Lactobacillus plantarum-fermented chili sauce via web-based computational analysis

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