Comparative lyophilized platelet-rich plasma wafer and powder for wound-healing enhancement: formulation, <i>in vitro</i> and <i>in vivo</i> studies

Yassin, Ghada E.; Dawoud, Marwa H. S.; Wasfi, Reham; Maher, Ahmed; Fayez, Ahmed M.

doi:10.1080/03639045.2019.1620269

Cited by 18 publications

(11 citation statements)

References 29 publications

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“…In recent years, several studies have been carried out to evaluate the stability of freeze-dried hemoderivative products. 22,23 Although remarkable results were observed in these studies, the lyophilized products were stored at temperatures under 4°C for their maintenance. In this study, it has been shown that lyophilized PRGF eye drops can be stored at RT for up to 3 months preserving their biological features like those of fresh PRGF eye drops.…”

Section: Discussionmentioning

confidence: 97%

Stability of freeze-dried plasma rich in growth factors eye drops stored for 3 months at different temperature conditions

Anitua

Fuente

Muruzábal

et al. 2020

European Journal of Ophthalmology

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Purpose: The purpose of this study was to analyze the biological content and activity of freeze-dried plasma rich in growth factors eye drops after their storage at 4°C and at room temperature for 3 months with respect to fresh samples (time 0). Methods: Plasma rich in growth factors was obtained after blood centrifugation from three healthy donors. After platelet activation, the obtained plasma rich in growth factors eye drops were lyophilized alone or in combination with lyoprotectant (trehalose), then they were stored for 3 months at room temperature or at 4°C. Several growth factors were analyzed at each storage time and condition. Furthermore, the proliferative and migratory potential of freeze-dried plasma rich in growth factors eye drops kept for 3 months at different temperature conditions was evaluated on primary human keratocytes. Results: The different growth factors analyzed maintained their levels at each time and storage condition. Freeze-dried plasma rich in growth factors eye drops stored at room temperature or 4°C for 3 months showed no significant differences on the proliferative activity of keratocytes in comparison with fresh samples. However, the number of migratory human keratocytes increased significantly after treatment with lyophilized plasma rich in growth factors eye drops kept for 3 months compared to those obtained at time 0. No significant differences were observed between the freeze-dried plasma rich in growth factors eye drops whether mixed or not with lyoprotectant. Conclusion: Freeze-dried plasma rich in growth factors eye drops preserve the main growth factors and their biological activity after storage at room temperature or 4°C for up to 3 months. Lyophilized plasma rich in growth factors eye drops conserve their biological features even without the use of lyoprotectants for at least 3 months.

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Section: Discussionmentioning

confidence: 97%

Stability of freeze-dried plasma rich in growth factors eye drops stored for 3 months at different temperature conditions

Anitua

Fuente

Muruzábal

et al. 2020

European Journal of Ophthalmology

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“…Wang et al [29] fabricated an antibacterial against S. aureus, P. aeruginosa and C. albicans composite dressing using chitosan/silk fibroin nanosilver loaded with PRP (prepared through double spinning), and assessed the physical and mechanical properties of the device, protein release, biological safety (silver content in organs) and efficacy in infectious wound treatment (CTS-SF/Ag/SA/PRP) in vitro and in vivo. In wound healing studies, Yassin et al [30] compared PRP wafers (carboxymethyl cellulose, CMC) and powder and antibacterial activities against Gram-negative bacteria. FD-wafers were proposed as PRP delivery systems: they showed better results than PRP powder in a rat wound model, in terms of antimicrobial efficacy, wound size measurements and histopathological analyses.…”

Section: Wound Managementmentioning

confidence: 99%

“…Moisture balance is beneficial to ensure appropriate wound care and can be achieved by combining PRP with biomaterials commonly used for this purpose in the manufacture of wound dressings. Combination products obtained through the mixture of FD-PRP with alginate [31], CMC [30] or gelatin [32] facilitate moisture preservation in the wound bed while serving as vehicles for sustained release, to match with the biological requirements of the wound [31].…”

Section: Wound Managementmentioning

confidence: 99%

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

Andı́a

Perez‐Valle

Amo

et al. 2020

IJMS

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The complex biology of platelets and their involvement in tissue repair and inflammation have inspired the development of platelet-rich plasma (PRP) therapies for a broad array of medical needs. However, clinical advances are hampered by the fact that PRP products, doses and treatment protocols are far from being standardized. Freeze-drying PRP (FD-PRP) preserves platelet function, cytokine concentration and functionality, and has been proposed as a consistent method for product standardization and fabrication of an off-the-shelf product with improved stability and readiness for future uses. Here, we present the current state of experimental and clinical FD-PRP research in the different medical areas in which PRP has potential to meet prevailing medical needs. A systematic search, according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analyses) guidelines, showed that research is mostly focused on wound healing, i.e., developing combination products for ulcer management. Injectable hydrogels are investigated for lumbar fusion and knee conditions. In dentistry, combination products permit slow kinetics of growth factor release and functionalized membranes for guided bone regeneration.

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“…b. HPLC analysis: This study analyzes the data and separates the analyte to know the components' purity and mobile phase selected based on the drug's solubility [42]. c. FTIR studies: In this study, the compatibility, i.e., any chemical interaction between the polymer and drug, can be detected by Fourier Transform Infrared Spectroscopy (FTIR) instrument [3,43,44].…”

Section: Characterization Studies a X-ray Diffraction (Xrd)mentioning

confidence: 99%

A Review of Diabetic Foot Ulcer Infections and Lyophilized Wafer Formulation

Jani

Puri

Ubana

2021

JPRI

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Diabetic foot difficulties are the most usually occurring problems globally, resulting in economic disasters for the patients, families, and society. In patients with diabetes, the risk of emerging foot ulcers is 25% high. It has also been in the record that one lower limb amputation occurs every 30 seconds in patients with diabetes worldwide. Novel methods of drug delivery and wound dressing have to develop to solve the lower limb amputation crisis. One such novel method is "Lyophilized wafer formulation." It is an upcoming medicated dressing material that can enhance wound healing and the potential to ingest vast quantities of exudates from Chronic wounds. That can have been formulating by lyophilizing hydrogel of absorbent polymers such as Calcium Alginate, Carrageenan, Thiolated Chitosan, and plasticizer to enhance flexibility withstand the day to day mechanical stress, covered with some adhesive and protective backing layer. Unless it passes evaluation tests such as Fourier-transform infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), Scanning electron microscopy (SEM), Exudate handling property, Folding endurance, In-vitro, In-vivo drug release profile, and Gamma-irradiation sterilizes wafer formulation, and it should not administrate directly. Lyophilized wafer formulation will be the most acceptable medicated dressing material in the future that will be useful to treat the normal wound. The wound formation because of diabetic foot ulcer (DFU) infections as there will be site-specific delivery of the drug, packed with an advantage to self administer and easy termination of the drug that can achieve just by removing the wafer case of drug toxicity.

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Comparative lyophilized platelet-rich plasma wafer and powder for wound-healing enhancement: formulation, in vitro and in vivo studies

Cited by 18 publications

References 29 publications

Stability of freeze-dried plasma rich in growth factors eye drops stored for 3 months at different temperature conditions

Stability of freeze-dried plasma rich in growth factors eye drops stored for 3 months at different temperature conditions

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

A Review of Diabetic Foot Ulcer Infections and Lyophilized Wafer Formulation

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