Comparative lectin histochemistry on the murine respiratory tract and primary olfactory pathway using a fully automated staining procedure

Keller, Lea-Adriana; Niedermeier, Sophia; Claassen, Lars; Popp, Andreas

doi:10.1016/j.acthis.2022.151877

Cited by 10 publications

(14 citation statements)

References 40 publications

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“…This emphasizes the broad diversity of glycoconjugates expressed in the VNO of the fossorial water vole. This contrasts with findings observed in rats (Salazar and Sánchez Quinteiro 1998) and mice (Keller et al 2022), where the vomeronasal glands are UEA positive but LEA negative.…”

Section: Lectin Histochemical and Immunohistochemical Stainingscontrasting

confidence: 99%

“…Regarding the respiratory epithelium, it Is noteworthy how in the fossorial water vole the UEA lectin produces strong labeling, consistent with findings in mice with the same lectin (Lundh et al 1989; Mendoza and Khünel 1991). Moreover, the lack of reactivity in the respiratory epithelium of the water vole against LEA aligns with observations by Keller et al (2022) in the respiratory epithelium of mice.…”

Section: Discussionsupporting

confidence: 86%

“…These were enhanced by lectin-histochemical labeling and immunohistochemical staining. Results obtained here on the anatomy and neurochemistry of the fossorial water vole VNO were compared with those results obtained in other rodents such as rats (Salazar and Sánchez Quinteiro 1998), mice (Keller et al 2022) and capybaras (Torres et al 2020), or carnivores like minks (Salazar et al 1998) and fox (Ortiz-Leal et al 2020) to go deeper on its differentiation and functionality levels. This will provide the basis for future studies focused on assessing the physiological effects of kairomones on individuals, and ultimately explore practices aimed at containing the growth and spread of wild populations.…”

Section: Introductionmentioning

confidence: 85%

“…This suggests that the glycoconjugates detected by them play a key role in recognizing and processing specific chemical signals. Both UEA and LEA also stain the sensory epithelium in rats (Lundh et al 1989; Salazar and Sánchez Quinteiro 1998) and mice (Salazar and Sánchez Quinteiro 2003; Keller et al 2022). However, a key difference exists compared to the fossorial water vole: there is a clear apical-basal zonation in the sensory epithelium in both species, especially in mice.…”

Section: Discussionmentioning

confidence: 99%

“…LEA has a maximum affinity for N-acetylglucosamine. LEA has served as a comprehensive marker for both the MOS and VNS in rodents such as rats (Salazar and Sánchez Quinteiro 1998), mice (Keller et al 2022) capybaras (Torres et al 2020), and carnivores like minks (Salazar et al 1998) and meerkats (Torres et al 2021).…”

Section: Methodsmentioning

confidence: 99%

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Do Fossorial Water Voles have a Functional Vomeronasal Organ? A Histological and Immunohistochemical Study

Ruiz-Rubio,

Ortiz-Leal,

Torres

et al. 2023

Preprint

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The fossorial water vole,Arvicola scherman, is a herbivorous rodent that cause significant agricultural damages. The application of cairomones and alarm pheromones emerges as a promising sustainable method to improve its integrated management. These chemical signals would induce stress responses that could interfere with the species regular reproductive cycles and induce aversive reactions, steering them away from farmlands and meadows. However, there is a paucity of information regarding the water vole vomeronasal system, both in its morphological foundations and its functionality, making it imperative to understand the same for the application of chemical communication in pest control.This study fills the existing gaps in knowledge through a morphological and immunohistochemical analysis of the fossorial water vole vomeronasal organ. The study is primarily microscopic, employing two approaches: histological, using serial sections stained with various dyes (Hematoxylin-Eosin, PAS, Alcian Blue, Nissl), and immunohistochemical, applying various markers that provide morphofunctional and structural information. These procedures have confirmed the presence of a functional vomeronasal system in fossorial water voles, characterized by a high degree of differentiation and a significant expression of cellular markers indicative of active chemical communication in this species.

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Section: Lectin Histochemical and Immunohistochemical Stainingscontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Do Fossorial Water Voles have a Functional Vomeronasal Organ? A Histological and Immunohistochemical Study

Ruiz-Rubio,

Ortiz-Leal,

Torres

et al. 2023

Preprint

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show abstract

Do fossorial water voles have a functional vomeronasal organ? A histological and immunohistochemical study

Ruiz‐Rubio,

Ortiz‐Leal,

Torres

et al. 2023

The Anatomical Record

View full text Add to dashboard Cite

The fossorial water vole, Arvicola scherman, is an herbivorous rodent that causes significant agricultural damages. The application of cairomones and alarm pheromones emerges as a promising sustainable method to improve its integrated management. These chemical signals would induce stress responses that could interfere with the species regular reproductive cycles and induce aversive reactions, steering them away from farmlands and meadows. However, there is a paucity of information regarding the water vole vomeronasal system, both in its morphological foundations and its functionality, making it imperative to understand the same for the application of chemical communication in pest control. This study fills the existing gaps in knowledge through a morphological and immunohistochemical analysis of the fossorial water vole vomeronasal organ. The study is primarily microscopic, employing two approaches: histological, using serial sections stained with various dyes (hematoxylin–eosin, Periodic acid‐Schiff, Alcian blue, Nissl), and immunohistochemical, applying various markers that provide morphofunctional and structural information. These procedures have confirmed the presence of a functional vomeronasal system in fossorial water voles, characterized by a high degree of differentiation and a significant expression of cellular markers indicative of active chemical communication in this species.

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SARS-CoV-2 induced changes in the glycosylation pattern in the respiratory tract of Golden Syrian hamsters

Barlang,

Mohl,

Blaurock

et al. 2023

Acta Histochemica

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Comparative lectin histochemistry on the murine respiratory tract and primary olfactory pathway using a fully automated staining procedure

Cited by 10 publications

References 40 publications

Do Fossorial Water Voles have a Functional Vomeronasal Organ? A Histological and Immunohistochemical Study

Do Fossorial Water Voles have a Functional Vomeronasal Organ? A Histological and Immunohistochemical Study

Do fossorial water voles have a functional vomeronasal organ? A histological and immunohistochemical study

SARS-CoV-2 induced changes in the glycosylation pattern in the respiratory tract of Golden Syrian hamsters

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