Comparative iTRAQ proteomics revealed proteins associated with spermatogenic arrest of cattleyak

Yu, Shumin; Cai, Xin; Sun, Lina; Zuo, Zhicai; Mipam, TserangDonko; Cao, Suizhong; Shen, Liuhong; Ren, Zhihua; Chen, Xi; Yang, Fang; Deng, Junliang; Ma, Xiaoping; Ya, Wang

doi:10.1016/j.jprot.2016.04.049

Cited by 33 publications

(44 citation statements)

References 46 publications

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“…The statistical significance of GO terms or KEGG pathway enrichment was determined by a hypergeometric test following Yu et al . [24]. Additionally, protein-protein interaction networks for DEPs were explored using the publicly available Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database [25].…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development

Wang

Liu

et al. 2017

PLoS ONE

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Heterostyly is a common floral polymorphism, but the proteomic basis of this trait is still largely unexplored. In this study, self- and cross-pollination of L-morph and S-morph flowers and comparison of embryo sac development in eggplant (Solanum melongena L.) suggested that lower fruit set from S-morph flowers results from stigma-pollen incompatibility. To explore the molecular mechanism underlying heterostyly development, we conducted isobaric tags for relative and absolute quantification (iTRAQ) proteomic analysis of eggplant pistils for L- and S-morph flowers. A total of 5,259 distinct proteins were identified during heterostyly development. Compared S-morph flowers with L-morph, we discovered 57 and 184 differentially expressed proteins (DEPs) during flower development and maturity, respectively. Quantitative real time polymerase chain reactions were used for nine genes to verify DEPs from the iTRAQ approach. During flower development, DEPs were mainly involved in morphogenesis, biosynthetic processes, and metabolic pathways. At flower maturity, DEPs primarily participated in biosynthetic processes, metabolic pathways, and the formation of ribosomes and proteasomes. Additionally, some proteins associated with senescence and programmed cell death were found to be upregulated in S-morph pistils, which may lead to the lower fruit set in S-morph flowers. Although the exact roles of these related proteins are not yet known, this was the first attempt to use an iTRAQ approach to analyze proteomes of heterostylous eggplant flowers, and these results will provide insights into biochemical events taking place during the development of heterostyly.

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Section: Methodsmentioning

confidence: 99%

Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development

Wang

Liu

et al. 2017

PLoS ONE

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“…The sampling of cattleyak and yak, and preparing of testis from each animal was described in Reference [1] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…This data is related to DEPs from testis proteome of cattleyak and yak using LC–ESI-MS/MS [1] . All the DEPs were categorized by GO and KEGG analysis.…”

Section: Datamentioning

confidence: 99%

“…All the DEPs were subjected to functional classification by Gene Ontology (GO) analysis and gene-pathway annotation by Kyoto Encyclopedia of Genes and Genomes (KEGG). The comparative testis proteome dataset here can shed new light on the molecular characteristics of male infertility of cattleyak on proteome level, “Comparative iTRAQ proteomics revealed proteins associated with spermatogenic arrest of cattleyak” [1] .…”

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confidence: 99%

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Comparative testis proteome dataset between cattleyak and yak

et al. 2016

Self Cite

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Cattleyak are hybrid between cattle and yak, which exhibit equivalent adaptability on the Qinghai-Tibetan Plateau as yak and much higher capability in economic traits. However, the F1 males of cattleyak are infertile due to spermatogenic arrest and this greatly restricts the effective utilization of this hybrid. In this data article, differentially expressed proteins (DEPs) were identified from testis proteome of cattleyak and yak using high-performance liquid chromatography–electrospray tandem mass spectrometry (LC–ESI-MS/MS). All the DEPs were subjected to functional classification by Gene Ontology (GO) analysis and gene-pathway annotation by Kyoto Encyclopedia of Genes and Genomes (KEGG). The comparative testis proteome dataset here can shed new light on the molecular characteristics of male infertility of cattleyak on proteome level, “Comparative iTRAQ proteomics revealed proteins associated with spermatogenic arrest of cattleyak” [1].

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“…Different methylation statuses of several candidate genes in cattle–yak testes have also been reported (Gu et al, ; Li, Luo, et al, ; Liu et al, ; Wang et al, ). Furthermore, proteome, transcriptome and microRNA analyses have been recently conducted on cattle–yak testes (Cai et al, ; Sun et al, ; Xu et al, ; Yu et al, ). However, the genetic cause of the male cattle–yak infertility is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Abnormal functions of Leydig cells in crossbred cattle–yak showing infertility

Sato

Kuriwaki

Hagino

et al. 2020

Reprod Domestic Animals

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In Mongolia, yak (Bos grunniens) are able to live in alpine areas and their products greatly influence the lives of the local people. Increased vigour in hybridized yak and cattle can offer benefits for livestock farmers. However, male hybrids show reproductive defects resulting from spermatogenesis arrest, affecting the conservation and maintenance of dominant traits in the next generation. The underlying mechanisms involved in hybrid cattle–yak infertility have recently been investigated; however, the genetic cause is still unclear. Androgens and androgen receptor (AR) signalling are required for spermatogenesis. We, therefore, evaluated the expression of AR, 3β‐hydroxysteroid dehydrogenase (3βHSD) and 5α‐reductase 2 (SRD5A2) in Leydig cells to investigate their function in cattle–yak spermatogenesis. Testicular tissues from yaks (1–3 years old) and hybrids (F1–F3, 2 years old) were collected and subjected to immunohistochemistry and image analyses to investigate the expression of each parameter in the Leydig cells. After maturation at 2 years, the expression levels of AR increased and the levels of 3βHSD decreased, but the SRD5A2 levels remained constant in yak. However, the cattle–yak hybrid F2 showed immature testicular development and significantly different expression levels of AR and 3βHSD compared with mature yak. These results suggest that the decreased expression of AR and increased expression of 3βHSD in the Leydig cells of cattle–yak hybrid testes may represent one of the causes of infertility. Our study might help in solving the problem of infertility in crossbreeding.

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Comparative iTRAQ proteomics revealed proteins associated with spermatogenic arrest of cattleyak

Cited by 33 publications

References 46 publications

Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development

Comparative proteomic analysis of eggplant (Solanum melongena L.) heterostylous pistil development

Comparative testis proteome dataset between cattleyak and yak

Abnormal functions of Leydig cells in crossbred cattle–yak showing infertility

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