Comparative iTRAQ proteomic profiling of susceptible and resistant apple cultivars infected by Alternaria alternata apple pathotype

Ni, Weichen; Zhu, Longming; Sha, Renhe; Tao, Jianmin; Cai, Baiyan; Wang, Sanhong

doi:10.1007/s11295-017-1104-5

Cited by 10 publications

(7 citation statements)

References 66 publications

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“…Another interesting observation made in our study was the decrease in fucose and especially rhamnose at high altitude, with the simultaneous accumulation of (+)-neomenthol dehydrogenase (SDR), an enzyme active in reduction-oxidation-processes of the plant cell 22 (Figs. 3, 6; Supplementary Table 1).…”

Section: Discussionmentioning

confidence: 62%

Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

Karagiannis

Michailidis

Tanou³

et al. 2020

Hortic Res

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Apple (Malus domestica Borkh) is an important fruit crop cultivated in a broad range of environmental conditions. Apple fruit ripening is a physiological process, whose molecular regulatory network response to different environments is still not sufficiently investigated and this is particularly true of the peel tissue. In this study, the influence of environmental conditions associated with low (20 m) and high (750 m) altitude on peel tissue ripening was assessed by physiological measurements combined with metabolomic and proteomic analyses during apple fruit development and ripening. Although apple fruit ripening was itself not affected by the different environmental conditions, several key color parameters, such as redness and color index, were notably induced by high altitude. Consistent with this observation, increased levels of anthocyanin and other phenolic compounds, including cyanidin-3-O-galactoside, quercetin-3-O-rhamnoside, quercetin-3-O-rutinoside, and chlorogenic acid were identified in the peel of apple grown at high altitude. Moreover, the high-altitude environment was characterized by elevated abundance of various carbohydrates (e.g., arabinose, xylose, and sucrose) but decreased levels of glutamic acid and several related proteins, such as glycine hydroxymethyltransferase and glutamate-glyoxylate aminotransferase. Other processes affected by high altitude were the TCA cycle, the synthesis of oxidative/defense enzymes, and the accumulation of photosynthetic proteins. From the obtained data we were able to construct a metabolite-protein network depicting the impact of altitude on peel ripening. The combined analyses presented here provide new insights into physiological processes linking apple peel ripening with the prevailing environmental conditions.

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Section: Discussionmentioning

confidence: 62%

Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

Karagiannis

Michailidis

Tanou³

et al. 2020

Hortic Res

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“…Interestingly, some of these were upregulated by both Penicillium spp., while for at least one of them, a higher induction rate was observed in fruit challenged with the apple non-pathogen P. digitatum , providing evidence for its role in the resistance of apple fruits to P. digitatum ( Buron-Moles et al, 2015 ). In another study aimed to determine the proteome profile of apple varieties susceptible and resistant to Alternaria alternata , another necrotrophic pathogen of apple, two major allergens, a glucanase (PR-2 family), and a thaumatin-like protein (PR-5 family) were associated with resistance to this disease ( Ni et al, 2017 ). In addition to apple fruits, allergen and major allergen PR proteins have been found to play a major role in the tolerance to B. cinerea infections on several other hosts such as strawberry or grape ( Monteiro et al, 2003 ; Haile et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…The current proteomic analysis further indicated that 2-Cys Prx was specifically up-accumulated in the O 3 -exposed fruits that had been inoculated with B. cinerea . 2-Cys Prx is a distinct subgroup within the family of peroxiredoxins (PRXs) that are associated with resistance of plants to both biotic and abiotic stress, while in apple fruits, their downregulation has been linked to A. alternata susceptibility ( Borges et al, 2013 ; Ni et al, 2017 ; Ojeda et al, 2018 ). Given that PRXs are highly conserved antioxidant proteins that scavenge reactive oxygen species (ROS) ( Zhang et al, 2019 ), it can be hypothesized that the increased abundance of 2-Cys Prx in O 3 -treated fruit reduces ROS level in the host and protects apple cells from the pathogen invasion.…”

Section: Discussionmentioning

confidence: 99%

Unraveling Interactions of the Necrotrophic Fungal Species Botrytis cinerea With 1-Methylcyclopropene or Ozone-Treated Apple Fruit Using Proteomic Analysis

Testempasis

Tanou²,

Minas

et al. 2021

Front. Plant Sci.

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Gray mold caused by the necrotrophic fungus Botrytis cinerea is one of the major postharvest diseases of apple fruit. The exogenous application of 1-methylcyclopropene (1-MCP) and gaseous ozone (O 3) is commonly used to ensure postharvest fruit quality. However, the effect of these treatments on the susceptibility of apple fruit to postharvest pathogens remains largely unknown. Herein, the effect of O 3 and 1-MCP treatments on the development of gray mold on apple fruit (cv. “Granny Smith”) was investigated. Artificially inoculated apple fruits, treated or not with 1-MCP, were subjected for 2 months to cold storage [0°C, relative humidity (RH) 95%] either in an O3-enriched atmosphere or in a conventional cold chamber. Minor differences between 1-MCP-treated and control fruits were found in terms of disease expression; however, exposure to ozone resulted in a decrease of disease severity by more than 50% compared with 1-MCP-treated and untreated fruits. Proteomic analysis was conducted to determine proteome changes in the mesocarp tissue of control and 1-MCP- or O3-treated fruits in the absence or in the presence of inoculation with B. cinerea. In the non-inoculated fruits, 26 proteins were affected by 1-MCP, while 51 proteins were altered by ozone. Dynamic changes in fruit proteome were also observed in response to B. cinerea. In O3-treated fruits, a significant number of disease/defense-related proteins were increased in comparison with control fruit. Among these proteins, higher accumulation levels were observed for allergen, major allergen, ACC oxidase, putative NBS-LRR disease resistance protein, major latex protein (MLP)-like protein, or 2-Cys peroxiredoxin. In contrast, most of these proteins were down-accumulated in 1-MCP-treated fruits that were challenged with B. cinerea. These results suggest that ozone exposure may contribute to the reduction of gray mold in apple fruits, while 1-MCP was not effective in affecting this disease. This is the first study deciphering differential regulations of apple fruit proteome upon B. cinerea infection and postharvest storage treatments, underlying aspects of host response related to the gray mold disease.

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“…Alternaria blotch disease caused by the necrotrophic pathogen, Alternaria alternata apple pathotype (A. alternata AP), is one of the most serious fungal diseases in East Asia affecting apple quality and yield via damaging young shoots, leaves and fruits, leading to huge economic losses [3]. Previously, most studies have mainly focused on A. alternata pathogenicity and toxicity [4][5][6], while only a few report on apple-pathogen interactions [7][8][9]. It indicated that the different resistance of apple varieties to A. alternata AP was associated with the expression divergence of pathogenesis-related (PR) proteins and some defense-related proteins [7].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Alternative Splicing in Two Contrasting Apple Cultivars Defense against Alternaria alternata Apple Pathotype Infection

Zhou

Zeng

et al. 2022

IJMS

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Alternaria blotch disease, caused by the Alternaria alternata apple pathotype (A. alternata AP), is one of the most serious fungal diseases in apples. Alternative splicing (AS), one of the pivotal post-transcriptional regulatory mechanisms, plays essential roles in various disease resistance responses. Here, we performed RNA-Seq for two apple cultivars (resistant cultivar ‘Jonathan’ (J) and susceptible cultivar ‘Starking Delicious’ (SD)) infected by A. alternata AP to further investigate their AS divergence. In total, 1454, 1780, 1367 and 1698 specifically regulated differential alternative splicing (DAS) events were detected in J36, J72, SD36 and SD72 groups, respectively. Retained intron (RI) was the dominant AS pattern. Conformably, 642, 764, 585 and 742 uniquely regulated differentially spliced genes (DSGs) were found during A. alternata AP infection. Comparative analysis of AS genes in differential splicing and expression levels suggested that only a small proportion of DSGs overlapped with differentially expressed genes (DEGs). Gene ontology (GO) enrichment analysis demonstrated that the DSGs were significantly enriched at multiple levels of gene expression regulation. Briefly, the specific AS was triggered in apple defense against A. alternata AP. Therefore, this study facilitates our understanding on the roles of AS regulation in response to A. alternata AP infection in apples.

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Comparative iTRAQ proteomic profiling of susceptible and resistant apple cultivars infected by Alternaria alternata apple pathotype

Cited by 10 publications

References 66 publications

Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

Decoding altitude-activated regulatory mechanisms occurring during apple peel ripening

Unraveling Interactions of the Necrotrophic Fungal Species Botrytis cinerea With 1-Methylcyclopropene or Ozone-Treated Apple Fruit Using Proteomic Analysis

Comparative Analysis of Alternative Splicing in Two Contrasting Apple Cultivars Defense against Alternaria alternata Apple Pathotype Infection

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