Comparative investigation of the role of the YadA, InvA, and PsaA genes in the pathogenicity of Yersinia pseudotuberculosis

Karataev, G. I.; Markov, Arseni; Sinyashina, L. N.; Miller, G. G.; Klitsunova, N. V.; Titova, Irina V.; Semin, E. G.; Гончарова, Н. И.; Pokrovskaya, M. S.; Амелина, И. П.; Amoako, Kingsley K.; Smirnov, G. B.

doi:10.3103/s0891416808040034

Cited by 3 publications

(1 citation statement)

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“…The expression data of the three genes were listed in Table 3 . In order to study of yadA (Yersinia adhesin A), researchers focus on the importance of the gene function on the pathogenicity of Yersinia and this gene was found to be a major virulence factor of Yersinia enterocolitica [ 56 - 58 ]. YadA is a trimeric autotransporter produced by Yersinia enterocolitica and it has manifold functions such as helping bacteria adhesion to host cells, promoting autoaggregation and protection of the bacteria from complement-mediated killing [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

Global transcriptome analysis of Mesorhizobium alhagi CCNWXJ12-2 under salt stress

et al. 2014

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BackgroundMesorhizobium alhagi CCNWXJ12-2 is a α-proteobacterium which could be able to fix nitrogen in the nodules formed with Alhagi sparsifolia in northwest of China. Desiccation and high salinity are the two major environmental problems faced by M. alhagi CCNWXJ12-2. In order to identify genes involved in salt-stress adaption, a global transcriptional analysis of M. alhagi CCNWXJ12-2 growing under salt-free and high salt conditions was carried out. The next generation sequencing technology, RNA-Seq, was used to obtain the transcription profiles.ResultsWe have compared the transcriptome of M. alhagi growing in TY medium under high salt conditions (0.4 M NaCl) with salt free conditions as a control. A total of 1,849 differentially expressed genes (fold change ≧ 2) were identified and 933 genes were downregulated while 916 genes were upregulated under high salt condition. Except for the upregulation of some genes proven to be involved in salt resistance, we found that the expression levels of protein secretion systems were changed under high salt condition and the expression levels of some heat shock proteins were reduced by salt stress. Notably, a gene encoding YadA domain-containing protein (yadA), a gene encoding trimethylamine methyltransferase (mttB) and a gene encoding formate--tetrahydrofolate ligase (fhs) were highly upregulated. Growth analysis of the three gene knockout mutants under salt stress demonstrated that yadA was involved in salt resistance while the other two were not.ConclusionsTo our knowledge, this is the first report about transcriptome analysis of a rhizobia using RNA-Seq to elucidate the salt resistance mechanism. Our results showed the complex mechanism of bacterial adaption to salt stress and it was a systematic work for bacteria to cope with the high salinity environmental problems. Therefore, these results could be helpful for further investigation of the bacterial salt resistance mechanism.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-014-0319-y) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Global transcriptome analysis of Mesorhizobium alhagi CCNWXJ12-2 under salt stress

et al. 2014

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Transcriptomic analysis of porcine PBMCs in response to Actinobacillus pleuropneumoniae reveals the dynamic changes of differentially expressed genes related to immuno-inflammatory responses

Jiang

Zhu

Liu

et al. 2018

Antonie van Leeuwenhoek

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Actinobacillus pleuropneumoniae is the cause of porcine pleuropneumonia, for which the mortality rate is high. Host peripheral blood is a body site for the immune clearance of pathogens mediated by release of inflammatory factors. However, "out of control" inflammatory factor release can contribute to host death. To further understand the changes in the transcription level of immune-related effectors, samples of peripheral blood mononuclear cells (PBMCs) collected from piglets at different stages of infection (0, 24 and 120 h) were sequenced on an Illumina HiSeq™ 4000 platform. We found 3818 differentially expressed genes (DEGs) in the 24 h-infection group compared to the 0 h-infection group (Pb24-Vs-Pb0). DEGs mainly involved in the Gene ontology and KEGG pathways that included nucleic acid metabolism regulation, cell growth, cell differentiation, and organ morphological maintenance were not significantly enriched (P > 0.05). However, DEGs associated with protein kinase activity, receptor activation, metabolism, local adhesion and immune inflammatory responses were significantly enriched in Pb120-Vs-Pb24 (P < 0.05), as were those related to the T cell receptor signalling pathway, with most being down-regulated compared to the preceding stage (Pb24-Vs-Pb0). In PBMCs there were some changes in glucose metabolism, local adhesion and the immune inflammatory response (Pb120-Vs-Pb0). In addition, up-regulated DEGs, such as IL8, IL1β, and CCL2, and were significantly enriched in immune-inflammatory related pathways compared to the uninfected stage, although they began to decline after 24 h.

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Sequence analysis of the yadA, inv, and ail genes and their expression in the main and nonmain Yersinia pestis subspecies and Yersinia pseudotuberculosis

et al. 2010

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Comparative investigation of the role of the YadA, InvA, and PsaA genes in the pathogenicity of Yersinia pseudotuberculosis

Cited by 3 publications

References 20 publications

Global transcriptome analysis of Mesorhizobium alhagi CCNWXJ12-2 under salt stress

Global transcriptome analysis of Mesorhizobium alhagi CCNWXJ12-2 under salt stress

Transcriptomic analysis of porcine PBMCs in response to Actinobacillus pleuropneumoniae reveals the dynamic changes of differentially expressed genes related to immuno-inflammatory responses

Sequence analysis of the yadA, inv, and ail genes and their expression in the main and nonmain Yersinia pestis subspecies and Yersinia pseudotuberculosis

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