Sequence analysis of the yadA, inv, and ail genes and their expression in the main and nonmain Yersinia pestis subspecies and Yersinia pseudotuberculosis

Eroshenko, G. A.; Odinokov, G. N.; Куклева, Л. М.; Krasnov, Ya. M.; Кутырев, Владимир

doi:10.1134/s1022795410060025

Cited by 7 publications

(7 citation statements)

References 18 publications

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“…caucasica, altaica, hissarica ) instead of Phe and additional substitution of Ser in the position 137 ( Y. pestis Pestoides F, Y. pestis subsp. caucasica ) (Eroshenko et al, 2010 ). Alignments of Ail from Y. pestis ( y1324 ) with Ail from Y. pseudotuberculosis IP 32953, Y. enterocolitica 8081c, and three other Y. pestis Ail homologs ( y1682, y2446 , and y2034 ) are depicted.…”

Section: Primary Secondary and Tertiary Structure Of Ailmentioning

confidence: 99%

“…Analysis of the ail nucleotide sequence reveals differences among the Y. pestis isolates that result in amino acid variation in the polypeptide chain (Eroshenko et al, 2010 ). Two variable sites in the gene can be identified: (1) a missense mutation in position 376 that results in a codon change from GTT to TTT and consequently a substitution of Val126 ( Y. pestis Pestoides F, Angola, Y. pestis subsp.…”

Section: Primary Secondary and Tertiary Structure Of Ailmentioning

confidence: 99%

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Yersinia pestis Ail: multiple roles of a single protein

Kolodziejek¹,

Hovde²,

Minnich³

2012

Front. Cell. Inf. Microbio.

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Yersinia pestis is one of the most virulent bacteria identified. It is the causative agent of plague—a systemic disease that has claimed millions of human lives throughout history. Y. pestis survival in insect and mammalian host species requires fine-tuning to sense and respond to varying environmental cues. Multiple Y. pestis attributes participate in this process and contribute to its pathogenicity and highly efficient transmission between hosts. These include factors inherited from its enteric predecessors; Y. enterocolitica and Y. pseudotuberculosis, as well as phenotypes acquired or lost during Y. pestis speciation. Representatives of a large Enterobacteriaceae Ail/OmpX/PagC/Lom family of outer membrane proteins (OMPs) are found in the genomes of all pathogenic Yersiniae. This review describes the current knowledge regarding the role of Ail in Y. pestis pathogenesis and virulence. The pronounced role of Ail in the following areas are discussed (1) inhibition of the bactericidal properties of complement, (2) attachment and Yersinia outer proteins (Yop) delivery to host tissue, (3) prevention of PMNL recruitment to the lymph nodes, and (4) inhibition of the inflammatory response. Finally, Ail homologs in Y. enterocolitica and Y. pseudotuberculosis are compared to illustrate differences that may have contributed to the drastic bacterial lifestyle change that shifted Y. pestis from an enteric to a vector-born systemic pathogen.

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Section: Primary Secondary and Tertiary Structure Of Ailmentioning

confidence: 99%

Section: Primary Secondary and Tertiary Structure Of Ailmentioning

confidence: 99%

Yersinia pestis Ail: multiple roles of a single protein

Kolodziejek¹,

Hovde²,

Minnich³

2012

Front. Cell. Inf. Microbio.

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“…один из этих генов -y1324, кодирует белок с мо-лекулярной массой 17,46 кда (без сигнальной после-довательности), на 99 % идентичный Ail Y. pseudotuberculosis и на 70 % -Y. enterocolitica [41]. анализ нуклеотидной последовательности гена ail Y. pestis выявил штаммовые различия, которые отражаются в вариациях аминокислот в полипеп-тидной цепи белка [3]. идентифицированы два ва-риабельных сайта в гене ail.…”

unclassified

“…у штаммов основного и улэгейского подвидов присутствует миссенс-мутация в позиции 376 (смена кодона GTT на TTT), приво-дящая к включению аминокислоты Phe вместо Val в 138 положении белка Ail. у штаммов кавказского подвида в гене ail выявлена вставка триплета AGT в позиции 408, следствием которой является вклю-чение остатка серина в 148 позицию полипептидной цепи белка [3].…”

unclassified

“…так, штаммы Y. pestis, имеющие укороченную коровую часть лпс, увели-чивают чувствительность к сыворотке человека [5,21]. наблюдаемые вариации аминокислот в поли-пептидной цепи Ail в различных штаммах Y. pestis, по-видимому, несущественны для обеспечения ре-зистентности к сыворотке, поскольку все изученные штаммы обладали одинаковой резистентностью к нормальной кроличьей сыворотке [3]. механизм устойчивости возбудителя чумы к сы-воротке, обусловленной Ail, пока неясен.…”

unclassified

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Adhesines of the Plague Agent

Куклева¹

2018

Problemy Osobo Opasnykh Infektsii

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удк 616.98:579.842.23 л.м.куклева адгеЗинЫ воЗБудителя чумЫ ФКУЗ «Российский научно-исследовательский противочумный институт «Микроб», Саратов, Российская Федерация возбудитель чумы обладает комплексом адгезинов, обеспечивающих прикрепление возбудителя к клеткам-мишеням в организме хозяина и во многом определяющих начало, характер и течение болезни. наличие адгези-нов обеспечивает транслокацию эффекторных белков в клетки-мишени млекопитающих. в обзоре представлены литературные данные как о наиболее изученных адгезинах Yersinia pestis (белках Ail и pH6 антиген), так и о недавно выявленных аутотранспортных белках различных классов, участвующих в процессах адгезии (YadBC, Yaps, Ilp). описано их значение для патогенеза чумы, генетическая детерминированность, структура и локализа-ция в клетке. отмечено, что адгезины возбудителя чумы действуют на разных стадиях инфекционного процесса, выполняют множественные функции, участвуют не только в процессах прикрепления к клеткам хозяина, но так-же обеспечивают устойчивость к действию иммунных механизмов хозяина.Ключевые слова: возбудитель чумы, адгезины, патогенность.

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Presence of the Oriental Rat Flea (Siphonaptera: Pulicidae) Infesting an Endemic Mammal and Confirmed Plague Circulation in a Forest Area of Madagascar

Harimalala

Rahelinirina

Girod

2020

Journal of Medical Entomology

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Abstract The Oriental rat flea, Xenopsylla cheopis (Rothschild 1903), is a cosmopolitan flea usually found infesting domestic rats. This flea is a well-known major human plague vector in Madagascar. As part of field sampling, fleas and small mammals were collected in the village of South Andranofeno and the natural reserve of Sohisika, two sites of the district of Ankazobe, located in the Central Highlands of Madagascar. Rats inside houses and forest small mammals were trapped using Besancon Technical Services and pitfall traps, respectively. Their fleas were collected and preserved for laboratory works. Collected fleas from the village and forest belonged to five species, which were X. cheopis, Synopsyllus fonquerniei (Wagner and Roubaud 1932) (Siphonaptera: Pulicidae), Echidnophaga gallinacea (Westwood 1875) (Siphonaptera: Pulicidae), Ctenocephalides felisstrongylus (Jordan 1925) (Siphonaptera: Pulicidae), Pulex irritans (Linnaeus 1758) (Siphonaptera: Pulicidae). After sampling in the forest zone, one specimen of X. cheopis was unexpectedly collected while infesting an endemic tenrec Setifer setosus (Schreber 1777) (Afrosoricida: Tenrecidae). Polymerase chain reaction (PCR) diagnosis on all collected fleas allowed detecting plague bacterium Yersinia pestis (Lehmann and Neumann 1896) (Enterobacterales: Yersiniaceae) on nine specimens of the endemic flea S. fonquerniei collected inside forest. The presence of the oriental rat flea in forest highlights the connection between human and wild environments due to animal movements and the fact that the rat flea can infest various hosts. As only one specimen of X. cheopis was collected on S. setosus, we hypothesize that flea was carried from the village to forest. Yersinia pestis infection of forest fleas outlines plague circulation in this sylvatic area.

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Sequence analysis of the yadA, inv, and ail genes and their expression in the main and nonmain Yersinia pestis subspecies and Yersinia pseudotuberculosis

Cited by 7 publications

References 18 publications

Yersinia pestis Ail: multiple roles of a single protein

Yersinia pestis Ail: multiple roles of a single protein

Adhesines of the Plague Agent

Presence of the Oriental Rat Flea (Siphonaptera: Pulicidae) Infesting an Endemic Mammal and Confirmed Plague Circulation in a Forest Area of Madagascar

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