Comparative in vitro study of the cytotoxicity of gelatine and alginate to human umbilical cord mesenchymal stem cells

Abstract

Background: Mesenchymal stem cells (MSCs) and scaffold combination constitute a promising approach currently adopted for tissue engineering. Umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are easily obtained and non-invasive. Gelatine and alginate constitute a biocompatible natural polymer scaffold. At present, a cytotoxicity comparison of gelatine and alginate to hUC-MSCs is not widely conducted Purpose: This study aimed to compare the cytotoxicity of gelatine and alginate in hUC-MSCs in vitro. Methods: Isolation and culture were performed on hUC-MSCs derived from healthy full-term neonates. Flow Cytometry CD90, CD105 and CD73 phenotype characterization was performed in passage 4. 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was performed to measure the cytotoxicity. The three sample groups were: (T1) hUC-MSCs with α-MEM (alpha-minimum essential medium) solution as control; (T2) hUC-MSCs with gelatine; (T3) hUC-MSCs with alginate Results: Flow cytometry of hUC-MSCs displayed positive CD90, CD105 and CD73 surface markers. Gelatine and alginate had no effect on the viability of hUC-MSCs and no statistically significant difference (p>0.05) of cytotoxicity between gelatine and alginate to hUC-MSCs. Conclusion: Gelatine and alginate proved to be non-toxic to hUC-MSCs in vitro.

Swelling‐Dependent Shape‐Based Transformation of a Human Mesenchymal Stromal Cells‐Laden 4D Bioprinted Construct for Cartilage Tissue Engineering

“…[32][33][34][35] Alginate is another natural hydrogel which is extensively used in bioprinting in general [36][37][38][39][40][41] and in cartilage engineering in particular. [39,[42][43][44] That is because it is non-toxic, [45,46] low-immunogenic, [47] and can gelate into a 3D structure upon exposure to Ca 2+ ions. [48,49] Here, we report an advanced 4D biofabrication method based on the differential swelling of a multi-material smart hydrogelbased bioink, with a special focus on cartilage TE.…”

“…[ 32–35 ] Alginate is another natural hydrogel which is extensively used in bioprinting in general [ 36–41 ] and in cartilage engineering in particular. [ 39,42–44 ] That is because it is non‐toxic, [ 45,46 ] low‐immunogenic, [ 47 ] and can gelate into a 3D structure upon exposure to Ca 2+ ions. [ 48,49 ]…”

Lipopolysaccharide’s Cytotoxicity on Human Umbilical Cord Mesenchymal Stem Cells

“…There is a higher interest in searching the prospects of human umbilical cord mesenchymal stem cells (HUCMSC) because this stem cell can be isolated and expanded easily in large quantities, and non-invasive compared to commonly used bone marrow mesenchymal stem cells [18]. There are studies about other MSC sources, but little information was available regarding HUCMSC and its potential use in pulpal and periapical regeneration.…”

Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells

“…Although human bone marrow mesenchymal stem cells are more popular, there are disadvantages, such as the requirement of general anesthesia regarding the invasive surgical procedure involved, and multiple procedures may be needed to acquire a sufficient number of cells [10]. Human umbilical cord mesenchymal stem cells (HUCMSC) are used in this study because they can be isolated and expanded easily in large quantities in vitro [2].…”