Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep

Kalyanasundaram, Aravindan; Jawahar, Shabnam; Ilangopathy, Manikkavasagan; Azhahianambi, P.; Raman, Muthusamy

doi:10.1016/j.exppara.2015.04.016

Cited by 15 publications

(10 citation statements)

References 38 publications

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“…These phenomena seem to indicate that Hcftt-2 could not be secreted for a long time or that the antigenicity is weak. A similar result was reported in other research [3]. As reported previously, antibodies may play a role in the immunoprotection against H. contortus [10,32,33].…”

Section: Discussionsupporting

confidence: 92%

“…Fecal egg output in H. contortus infections is probably the best in vivo phenotypic marker of resistance or protection [3,9]. In this research, goats immunized with rHcftt2 showed a significant 26.46% reduction in egg output, and the same vaccinated animals displayed a 32.22% reduction in the amount of adult H. contortus collected from the abomasa compared with the positive control group at the end of the trial.…”

Section: Discussionmentioning

confidence: 66%

“…Haemonchus contortus is a highly pathogenic blood-sucking gastrointestinal nematode of the abomasum of small ruminants. Infections with this nematode can cause anemia, weight loss, and even death [1][2][3][4]. Current methods of prevention and control depend on the use of anthelmintics [3].…”

Section: Introductionmentioning

confidence: 99%

“…Infections with this nematode can cause anemia, weight loss, and even death [1][2][3][4]. Current methods of prevention and control depend on the use of anthelmintics [3]. However, the appearance of anthelmintic-resistant strains, the environmental pollution, and the toxic residues in animal products has led to the urgent need for the development of more effective vaccines [5].…”

Section: Introductionmentioning

confidence: 99%

“…Until now, many native and recombinant proteins have been investigated in different immunization trials against H. contortus and most cases demonstrated variable reduction in fecal egg output and worm burdens [3,[6][7][8][9]. DNA vaccines have also been tested and showed partial protection against H. contortus [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Immunization of Goats with Recombinant Protein 14-3-3 Isoform 2(rHcftt-2) Induced Moderate Protection against Haemonchus contortus Challenge

Jia

Tian

et al. 2020

Pathogens

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A previous study identified that isoform 2 (Hcftt-2) of the 14-3-3 protein of Haemonchus contortus (H. contortus) could suppress immune functions of goat peripheral blood mononuclear cells (PBMCs) and might be a potential vaccine target, as neutralization of the protein function may enhance anti-parasite immunity. In this research, the recombinant Hcftt-2 was evaluated for its immunoprotective efficacy against H. contortus infection in goats. Five experimental goats were immunized twice with rHcftt-2 along with Freund’s adjuvant. The five immunized goats and five nonimmunized goats (adjuvant only) were challenged with 5000 L3-stage H. contortus larvae after 14 days of second immunization. Five nonimmunized and uninfected goats (adjuvant only) were set as the uninfected group. A significant increase in the serum immunoglobin G(IgG) and serum IgA levels were identified in the rHcftt-2 immunized animals. The mean eggs per gram in feces (EPG) and the worm burdens of rHcftt-2 immunized group were reduced by 26.46% (p < 0.05) and 32.33%, respectively. In brief, immunization of goats with rHcftt-2 induced moderate protection against H. contortus challenge.

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