Comparative immunohistochemical study of proliferation and apoptosis in colorectal adenocarcinoma

Shyshkin, М. А.

doi:10.14739/2310-1237.2016.3.87497

Cited by 1 publication

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“…Moreover, there is a direct medium correlation between the low 53 expression level by epitheliocytes of adenomas and the grade of dysplasia (γ=0,53). According to the results of IHC study of caspase-3 (the marker of apoptotic degradation) it was established that the DCP are characterized by the low apoptosis level of epithelial and stromal cells: the median of caspase-3 expression by adenomas' epitheliocytes was 31,84 CUOD (19,53; 42,34), by hyperplastic polyps' epitheliocytes -16,99 CUOD (11,86; 39,85), (р>0,05); the median of caspase-3 expression by adenomas' stromal cells was 43,87 CUOD (25,47; 73,09), by hyperplastic polyps' stromal cells -28,64 CUOD (19,20; 30,71), (р˂0,05).…”

Section: Research Results and Discussionmentioning

confidence: 99%

“…3. Distal colonic polyps are characterized by the low level of apoptosis of epitheliocytes [Ме=31,84CUOD (19,53;42,34) in adenomas, Ме=16,99CUOD (11,86; 39,85) in hyperplastic polyps] and stromal cells [Ме=43,87CUOD (25,47; 73,09) in adenomas, Ме=28,64CUOD (19,20; 30,71) in hyperplastic polyps], that were evaluated by the caspase-3 immunohistochemical expression.…”

Section: Research Results and Discussionmentioning

confidence: 99%

“…Activation of cell proliferation mediated by proto-oncogenes combined with inactivation of apoptotic cell death mediated by tumor-suppressor genes is one of universal molecular characteristics of carcinogenesis [5]. The features of proliferation and apoptosis in CRC were studied in the number of studies [8][9][10] including the work carried out in the context of this study [11][12], while the question of features of proliferation and apoptosis in colon polyps which are precursors of CRC weren't elaborated.…”

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confidence: 99%

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Distal colonic polyps: immunohistochemical study of proliferation and apoptosis

Shyshkin

Khrystenko

2019

Morphologia

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colonic polyps: immunohistochemical study of proliferation and apoptosis. Zaporizhzhya state medical university, Zaporizhzhya, Ukraine ABSTRACT. Background. It's well known that colonic polyps can be precursors of colorectal cancer. But exact immunohistochemical parameters, including proliferation and apoptosis levels of polyps' cells, that may have prognostic value, still not known. Objective. To study the features of immunohistochemical expression of proliferation and apoptosis markers by distal colonic polyps' cells. Methods. Pathomorphological and immunohistochemical studies of biopsies of distal colonic polyps from 30 patients (the age ranged from 41 to 83 years) were carried out. Results. Distal colonic polyps are characterized by the medium proliferation level of epithelial cells [Me=62.40% (48.65; 76.23) for adenomas, Me=26.23% (22.19; 48.88) for hyperplastic polyps], the low proliferation level of stromal cells [Me=2.94% (1.23; 4.35) for adenomas, Me=1.18% (1.10; 1.86) for hyperplastic polyps], the low p53 expression level of epithelial and stromal cells [Me=2.39% (1.58; 8.26) and Me=1.23% (0.72; 1.49) for adenomas' cells, Me=0,00% (0,00; 1,47) and Me=0,00% (0,00; 0,05) for hyperplastic polyps' cells], and also by the low apoptosis level of epithelial and stromal cells [Me=31.84CUOD (19.53; 42.34) and Me=43.87CUOD (25.47; 73.09) for adenomas, Me=16.99CUOD (11.86; 39.85) and Me=28.64CUOD (19.20; 30.71) for hyperplastic polyps]. There are direct correlations between the expression levels of proliferation and apoptosis markers and also the dysplasia grade of the distal colonic adenomas: between the Ki-67 expression levels of epithelial and stromal cells and also the dysplasia grade (γ=0.65 and γ=0.70, respectively), between the p53 expression level of epithelial cells and the dysplasia grade (γ=0.53), between the caspase-3 expression levels of epithelial and stromal cells and also the dysplasia grade (γ=0.80 and γ=0.63, respectively). Moreover, there are direct correlations between the expression levels of proliferation and apoptosis markers of distal colonic polyps' epitheliocytes: between the Ki-67 and p53 expression levels (r=0.71 for adenomas, r=0.79 for hyperplastic polyps), between the Ki-67 and caspase-3 expression levels (r=0.61 for adenomas), between the p53 and caspase-3 expression levels (r=0.59 for adenomas, r=0.79 for hyperplastic polyps). Conclusion. These data indicate the close association between the processes of proliferation, accumulation of p53 protein, and apoptosis of the distal colonic polyps' cells.

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Section: Research Results and Discussionmentioning

confidence: 99%

Section: Research Results and Discussionmentioning

confidence: 99%