Comparative in vitro antibacterial activity of six root canal sealers against <scp>E</scp>nterococcus faecalis and <scp>P</scp>roteus vulgaris

Farmakis, Eleftherios-Terry R.; Kontakiotis, Evangelos G.; A, Tseleni-Kotsovili; Tsatsas, Vasilios G.

doi:10.1111/j.2041-1626.2012.00159.x

Cited by 15 publications

(13 citation statements)

References 24 publications

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“…Previous investigations pointed out that the original formulation of GuttaFlow does not present antimicrobial activity, 9,10 and thus the silver nanoparticles were solely responsible for promoting a preservative effect. 16 Silver particles present antimicrobial potential, which is manifested by anchorage to or penetration into the bacterial cell wall, and modulation of cellular signaling by dephosphorylating putative key peptide substrates on tyrosine residues.…”

Section: Discussionmentioning

confidence: 99%

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In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

et al. 2016

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In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers Abstract: The aim of this study was to evaluate whether the modification in the silver component is capable of providing GuttaFlow 2 with antibacterial activity against Enterococcus faecalis compared with epoxy resin-based (AH Plus) and zinc oxide and eugenol-based (Endofill) sealers. The antibacterial activity was evaluated using a reference strain of E. faecalis (ATCC 29212). Freshly mixed sealers were subjected to the agar diffusion test (ADT), while the direct contact test (DCT) was performed after materials setting. ADT results were obtained through measurements, in millimeters, of the inhibition zones promoted by the materials, using a digital caliper. In DCT, values of CFU/mL promoted by the three sealers were compared in three experimental periods (1 min, 1 h, and 24 h). The data were analyzed using Kruskal-Wallis and Dunn post-hoc tests (p < 0.05). In both ADT and DCT, GuttaFlow 2 presented no effect against E. faecalis, while Endofill and AH Plus showed similar inhibition zones. Endofill was the only material capable of reducing bacterial growth in DCT. In conclusion, modifications in the silver particle of GuttaFlow 2 did not result in a sealer with antibacterial effect against E. faecalis.

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Section: Discussionmentioning

confidence: 99%

“…faecalis, and Proteus vulgaris. 10 On the other hand, the incorporation of low concentrations of insoluble antibacterial nanoparticles in the GuttaFlow original formulation exhibited significant and stable antimicrobial properties.…”

mentioning

confidence: 99%

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

et al. 2016

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“…However, they had a weak antibacterial effect similar to that of calcium-hydroxide-based sealers. 26–28 To date, the development of a root canal sealer with long-term antibacterial activity and good biocompatibility remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

Bioactive mono-dispersed nanospheres with long-term antibacterial effects for endodontic sealing

Cheng

et al. 2017

J. Mater. Chem. B

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Endodontic sealers with antibacterial capability play an important role in preventing reinfection of an endodontically treated root canal and improving the long-term success of root canal treatment. However, current endodontic sealers rapidly lose their antibacterial properties after fixation. In this work, we designed and synthesized quaternized mono-dispersed bioactive nanospheres as a potential substrate for the development of a long-term antibacterial endodontic sealer with excellent cytocompatibility and biocompatibility. First, mono-dispersed silica-based bioactive glass nanospheres (SBG-NS) were prepared via a modified sol-gel process. Next, a series of quaternary ammonium methacrylate salts (QAMs) with broad antibacterial spectra were synthesized and grafted onto the surfaces of the SBG-NS via a two-step coupling approach. The antibacterial effect of the quaternary ammonium polymethacrylate (QAPM)-containing SBG-NS (SBG-QAPM) against persistent microorganisms associated with infected root canals was evaluated using a direct contact test. Evaluations of the SBG-QAPM cytocompatibility and biocompatibility were performed using LIVE/DEAD staining, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2, 5-tetrazoliumbromide (MTT) assay, and a calvarial implantation model. The results showed that the SBG-QAPMs had the strongest long-term antibacterial effect against the Enterococcus faecalis, Streptococcus mutans, and Streptococcus sanguis during the study period, the best cytocompatibility, and the lowest systemic inflammation compared to three commercial products: ProRoot MTA, Endomethasone C, and AH Plus. In addition, the SBG-QAPMs showed excellent stability in aqueous solution. This work indicates that the SBG-QAPMs are promising substrates for the development of long-term antibacterial endodontic sealers.

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“…Los resultados de los estudios de Wainsteim, descritos anteriormente, mostraron halos de inhibición provocados por AH Plus mayores al encontrado por el sellador Topseal, debido a que en dichos estudios se crearon pozos en la superfi cie del agar, de esta manera se obtuvo un contacto directo de los selladores y el microorganismo, mientras que en la presente investigación los selladores estaban contenidos en discos de papel cartulina que se pusieron en contacto con el agar sobre el cual se sembró el Enterococcus faecalis. Farmakis et al, en el año de 2012, demostraron la efi cacia antibacteriana de Topseal el mismo que provocó un halo de inhibición de 11 mm, Farmakis usó el método de difusión de agar por 24 horas a 37°C, para ello creó en la superfi cie del agar un pozo con el fi n de tener contacto directo entre el material de estudio y el medio de cultivo, usando una pipeta para un volumen de 0,3 ml, los selladores recién mezclados fueron introducidos en el pozo mediante una jeringa de insulina (15). Esta investigación encontró un halo nol posee efecto antimicrobiano, el eugenol tiene un efecto bactericida, acción que se ha atribuido a los fenoles por degeneración de las bacterias, hongos y formas vegetativas (10).…”

Section: Efi Cacia Antibacteriana De Tres Selladores Endodónticos Freunclassified

Eficacia antibacteriana de tres selladores endodónticos frente al Enterococcus faecalis

Veloz¹,

Abad-Coronel²,

Caparó³

2017

Rev Estomatol Herediana.

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Comparative in vitro antibacterial activity of six root canal sealers against Enterococcus faecalis and Proteus vulgaris

Cited by 15 publications

References 24 publications

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

In vitro antibacterial activity of a silicone-based endodontic sealer and two conventional sealers

Bioactive mono-dispersed nanospheres with long-term antibacterial effects for endodontic sealing

Eficacia antibacteriana de tres selladores endodónticos frente al Enterococcus faecalis

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