Comparative gonad protein and metabolite responses to a binary mixture of 2,4′-DDT and benzo(a)pyrene in the female green mussel Perna viridis

Qiu, Liguo; Song, Qinqin; Jiang, Xiu; Zhao, Hongwei; Chen, Hao; Zhou, Hailong; Han, Qian

doi:10.1007/s11306-016-1089-3

Cited by 9 publications

(5 citation statements)

References 54 publications

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“…Plots were made by the R's ggplot2[ 42]. The quality of the PLS-DA model was cross validated by permutation tests, with the R 2 value described the data fitness and Q 2 value described the predictive ability as reported[ 43]. For each metabolite, the Variable Importance in the Projection (VIP) score was calculated[ 44] to determine the most relevant metabolites by SBM substitution.…”

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confidence: 99%

WITHDRAWN: 1H NMR-based metabolomics investigation of dietary soybean meal substitution in hybrid sturgeon (Acipenser baerii ♀× A. schrenckii ♂)

Yue¹,

Wu²,

Ruan³

et al. 2019

Preprint

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Background: Soybean proteins is the most widely used alternative protein sources for fish meal in aquafeed. However, soybean meal (SBM) substitution imposes negative effects such as growth restriction and intestinal inflammation. Limited reports were concentrated on the metabolic alterations in fish. Results: A ten-week feeding trial was conducted to examine the effect of dietary SBM substitution on growth performance, serum biochemistry and metabolism in hybrid sturgeon. It was showed that 25% SBM substitution produced the highest weight gain, with hepatosomatic index reduced significantly in the 50% and 100% SBM substitution groups. Besides, SBM diet led to significant increase of the glutamic-pyruvic transaminase concentration in the 100% SBM diet group. Moreover, SBM diets higher than 50% resulted in hepatic injury of lipid droplet accumulation by histological analysis. 1H NMR method was applied to detect metabolite changes in liver and serum. Totally, 47 and 50 representative metabolites were screened in liver and serum, respectively. The subsequent PLS-DA analysis identified 23 in liver and 10 in serum of metabolites affected by SBM substitution through pairwise comparisons, respectively. The following pathway enrichment revealed 9 in liver and 17 in serum of perturbed pathways, respectively. Among them, pathway of phenylalanine, tyrosine and tryptophan biosynthesis with two differentiated metabolites (phenylalanine and tyrosine) involved deserved the highest impact factor, which indicated severe liver damage by SBM diets.

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confidence: 99%

WITHDRAWN: 1H NMR-based metabolomics investigation of dietary soybean meal substitution in hybrid sturgeon (Acipenser baerii ♀× A. schrenckii ♂)

Yue¹,

Wu²,

Ruan³

et al. 2019

Preprint

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“…PLSDA was then used for cluster analysis and to build a discriminant model. Permutation tests ( n = 200) were used to evaluate the validity of PLSDA models [ 69 ].…”

Section: Methodsmentioning

confidence: 99%

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Qiang

Tao

et al. 2021

BMC Genomics

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Background Dissolved oxygen (DO) in the water is a vital abiotic factor in aquatic animal farming. A hypoxic environment affects the growth, metabolism, and immune system of fish. Glycolipid metabolism is a vital energy pathway under acute hypoxic stress, and it plays a significant role in the adaptation of fish to stressful environments. In this study, we used multi-omics integrative analyses to explore the mechanisms of hypoxia adaptation in Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). Results The 96 h median lethal hypoxia (96 h-LH50) for GIFT was determined by linear interpolation. We established control (DO: 5.00 mg/L) groups (CG) and hypoxic stress (96 h-LH50: 0.55 mg/L) groups (HG) and extracted liver tissues for high-throughput transcriptome and metabolome sequencing. A total of 581 differentially expressed (DE) genes and 93 DE metabolites were detected between the CG and the HG. Combined analyses of the transcriptome and metabolome revealed that glycolysis/gluconeogenesis and the insulin signaling pathway were down-regulated, the pentose phosphate pathway was activated, and the biosynthesis of unsaturated fatty acids and fatty acid metabolism were up-regulated in GIFT under hypoxia stress. Conclusions The results show that lipid metabolism became the primary pathway in GIFT under acute hypoxia stress. Our findings reveal the changes in metabolites and gene expression that occur under hypoxia stress, and shed light on the regulatory pathways that function under such conditions. Ultimately, this information will be useful to devise strategies to decrease the damage caused by hypoxia stress in farmed fish.

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“…Partial least squares discriminant analysis (PLSDA) was then used for cluster analysis and to build a discriminant model. Permutation tests ( n = 200) were utilized to evaluate the validity of the PLSDA models (Qiu et al., 2016).…”

Section: Methodsmentioning

confidence: 99%

Untargeted LC–MS metabolomics approach reveals metabolic changes in genetically improved farmed tilapia (Oreochromis niloticus) with fatty liver induced by a high‐fat diet

et al. 2020

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Comparative gonad protein and metabolite responses to a binary mixture of 2,4′-DDT and benzo(a)pyrene in the female green mussel Perna viridis

Cited by 9 publications

References 54 publications

WITHDRAWN: 1H NMR-based metabolomics investigation of dietary soybean meal substitution in hybrid sturgeon (Acipenser baerii ♀× A. schrenckii ♂)

WITHDRAWN: 1H NMR-based metabolomics investigation of dietary soybean meal substitution in hybrid sturgeon (Acipenser baerii ♀× A. schrenckii ♂)

Multi-omics analysis reveals the glycolipid metabolism response mechanism in the liver of genetically improved farmed Tilapia (GIFT, Oreochromis niloticus) under hypoxia stress

Untargeted LC–MS metabolomics approach reveals metabolic changes in genetically improved farmed tilapia (Oreochromis niloticus) with fatty liver induced by a high‐fat diet

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