Comparative global transcription analysis of sodium hypochlorite, peracetic acid, and hydrogen peroxide on Pseudomonas aeruginosa

Abstract

Disinfectants are routinely used in hospitals and health care facilities for surface sterilization. However, the mechanisms by which these disinfectants kill and the extent to which bacteria, including Pseudomonas aeruginosa, are resistant remains unclear. Consequently, P. aeruginosa nosocomial infections result in considerable casualties and economic hardship. Previously, DNA microarrays were utilized to analyze the genome-wide transcription changes in P. aeruginosa after oxidative antimicrobial (sodium hypochlorite, peracetic acid, and hydrogen peroxide) exposure. Simultaneous analysis of these transcriptome datasets provided a comprehensive understanding of the differential responses to these disinfectants. An analysis of variance, functional classification analysis, metabolic pathway analysis, Venn diagram analysis, and principal component analysis revealed that sodium hypochlorite exposure resulted in more genome-wide changes than either peracetic acid or hydrogen peroxide exposures.

Mechanisms of Resistance to Chloramphenicol in Pseudomonas putida KT2440

“…This particular cytochrome oxidase complex shows high affinity for O 2 and is considered an adaptive trait to microaerobic conditions (12). Small and colleagues (57) found that P. aeruginosa downregulates Cbb3-encoding genes in response to oxidative agents such as sodium hypochlorite, peracetic acid, and hydrogen peroxide, as well as a bacteriostatic antibiotic known as fusidic acid (62). The authors attributed this trait to the decrease in the cellular respiratory function and the loss of metabolic energy, as had been previously found upon exposure to HOCl (4).…”

Efficacy of chlorine and peroxyacetic acid on reduction of natural microflora, Escherichia coli O157:H7, Listeria monocyotgenes and Salmonella spp. on mung bean sprouts

“…The disinfection efficacy of PAA is not subjected to pH fluctuations as that of chlorine (Buchholz and Matthews, 2010). In addition, the activity of PAA does not weaken as much as chlorine in the presence of organic matter (Small et al, 2007;Vandekinderen et al, 2009). PAA has also lesser detrimental impacts on human health and environment as compared to chlorine.…”

Efficacy of Chlorine, Chlorine Dioxide, and Peroxyacetic Acid in Reducing Salmonella Contamination in Wash Water and on Mangoes Under Simulated Mango Packinghouse Washing Operations

“…Commercially available peracetic acid-based disinfectants contain a considerable amount of hydrogen peroxide that also exhibits antimicrobial activity. PAA is not susceptible to peroxidases and is known to retain its antimicrobial activity in the presence of organic loads or food residues when compared to chlorine (Fatemi and Frank, 1999;Hilgren et al, 2007;Small et al, 2007). Although antimicrobial, the oxidizing ability of these disinfectants, particularly chlorine dioxide and PAA are also associated with discoloration and negative impact on fruit color (Wang et al, 2007;Du et al, 2009;Joshi et al, 2013;Van de Velde et al, 2016;Chen, 2017).…”