Comparative genotoxicity of quinolone and quinolonyl-lactam antibacterials in the in vitro micronucleus assay in Chinese hamster ovary cells

Gibson, David; Ma, Xin; Switzer, Adrian G.; Murphy, Vincent A.; Aardema, Marilyn J.

doi:10.1002/(sici)1098-2280(1998)31:4<345::aid-em7>3.0.co;2-h

Cited by 19 publications

(6 citation statements)

References 2 publications

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“…For a higher concentration of antibiotics, the number of MN increased about 5.5 to 7 fold with ciprofloxacin. Results obtained for NA are not in agreement with the negative in vitro MN results reported by Albertini et al (1995) and Gibson et al (1998) in hamster CHO-K cells exposed to this compound. In a review by Forte (1992), NA was reported to be negative in an in vitro chromosome aberration assay.…”

Section: Discussioncontrasting

confidence: 96%

“…A reduction in the mitotic index and fuzzy metaphases were observed at 50 mg/L of ciprofloxacin, indicating a cytotoxic effect of this compound. In previous studies, Albertini et al (1995) and Curry et al (1996) reported significant increases in both chromosome aberration and in MN induction in the same range of ciprofloxacin concentration (0.3-1.8 mM) than those observed by Gibson et al (1998) in CHO-K1 cells. These results indicated the higher genotoxicity of ENR and CIP in comparison with NA.…”

Section: Discussionmentioning

confidence: 67%

“…Ciprofloxacin was found in concentration ranging from 0.7 to 124.5 mg/L in hospital effluents and was assumed to be the main source of genotoxic effects measured with the UmuC test in these effluents (Hartmann et al, 1999). Fluoroquinolones have been reported to induce unscheduled DNA synthesis, DNA strand breakage, chromosome damage and micronuclei formation (Mc Queen et al, 1991;Holden et al, 1989;Bredberg et al, 1991;Ciaravinco et al, 1993;Curry et al, 1996;Gibson et al, 1998). In addition, FQs exert other toxic and genotoxic effects on animal and human cells.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, FQs exert other toxic and genotoxic effects on animal and human cells. For example, exposure of Chinese hamster ovary cells or Syrian hamster embryo cells to nalidixic acid (NA) and ciprofloxacin (CIP) induced cytotoxic effects and micronucleus induction (Gibson et al, 1998). In vitro studies with human lymphocytes exposed to enrofloxacin (ENR) and ciprofloxacin revealed an increase in the chromosomal aberrations, detected as chromatid and chromosome breaks and gaps (Gorla et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Khadra¹,

Pinelli²,

Lacroix³

et al. 2012

Ecotoxicology and Environmental Safety

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, et al.. Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test. Ecotoxicology and Environmental Safety, Elsevier, 2012, 76, pp.187-192. <10.1016/j.ecoenv.2011 a b s t r a c tThe genotoxicity of quinolone and fluroquinolones was assessed using the micronucleus (MN) test on Vicia faba roots by direct contact exposure to a solid matrix. Plants were exposed to quinolones (nalidixic acid) and fluoroquinolones (ciprofloxacin and enrofloxacin) alone or mixed with artificially contaminated soils. Four different concentrations of each of these antibiotics were tested (0.01, 0.1, 1 and 10 mg/Kg) for nalidixic acid and (0.005, 0.05, 0.5 and 5 mg/Kg) for ciprofloxacin and enrofloxacin. These antibiotics were also used in mixture. Exposure of Vicia faba plants to each antibiotic at the highest two concentrations showed significant MN induction. The lowest two concentrations had no significant genotoxic effect. The mixture of the three compounds induced a significant MN induction whatever the mixture tested, from 0.02 to 20 mg/Kg. The results indicated that a similar genotoxic effect was obtained with the mixture at 0.2 mg/Kg in comparison with each molecule alone at 5-10 mg/Kg. Data revealed a clear synergism of these molecules on Vicia faba genotoxicity.

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Section: Discussioncontrasting

confidence: 96%

Section: Discussionmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Khadra¹,

Pinelli²,

Lacroix³

et al. 2012

Ecotoxicology and Environmental Safety

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“…In the present study, the effects of OA on DNA were evaluated further, using the cytokinesisblock micronucleus (CBMN) test in CHO-K1 cells. This cell type has been used routinely in in vitro micronucleus (MN) screening assays for the detection of genotoxicants (Gibson et al, 1998;Erexson et al, 2001). The CBMN methodology is based on the inhibition of cytokinesis by cytochalasin B (cyt B), allowing distinction between cells which did not divide, mononucleated cells, and those which either divided once, binucleated cells (BN), or divided more that once, polynucleated cells (PN) (Fenech and Morley, 1985).…”

Section: Introductionmentioning

confidence: 99%

Aneugenic potential of okadaic acid revealed by the micronucleus assay combined with the FISH technique in CHO-K1 cells

Hégarat¹

2003

Mutagenesis

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Okadaic acid (OA) is a major toxin involved in diarrhetic shellfish poisoning in humans and has been shown to be both a potent tumor promoter in rodent skin and stomach and an inhibitor of serine/threonine protein phosphatases, specifically PP1 and PP2A. The research on the genotoxic potential of OA amounts to only a few studies, which give conflicting results. In order to evaluate the ability of OA to induce DNA damage, the cytokinesis-block micronucleus assay was performed in the CHO-K1 cell line. A statistically significant induction of micronuclei without strong cytotoxicity was obtained after a 24 h treatment with 20 (approximately 5-fold) and 30 nM (approximately 10-fold) OA. Then, in order to discriminate between a clastogenic or aneugenic effect of OA, the micronucleus assay was carried out in combination with fluorescence in situ hybridization (FISH) using a (TTAGGG)(n) DNA probe for centromere detection. FISH analysis showed that OA mainly induced centromere-positive micronuclei (68.9% induction with 20 nM OA and 77.0% with 30 nM). Therefore, OA can be considered aneugenic. Using the same assay, biotransformation of OA was studied after a 4 h treatment with and without metabolic activation. The results show that reactive metabolites of OA were generated with a significant increase in genotoxic potential. The relationship between the different components involved in the mitotic process and OA inhibition of protein phosphatase is also discussed.

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Toxicity and Genotoxicity of Three Antimicrobials Commonly Used in Veterinary Medicine

Magdaleno

Morettón

2017

Bull Environ Contam Toxicol

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The toxicity of chlortetracycline (CTC), oxytetracycline (OTC) and enrofloxacin (ENF) was tested on two green algal species: the international standard Pseudokirchneriella subcapitata and the native Argentine species Ankistrodesmus fusiformis. All three antibiotics inhibited the algal growth. The most sensitive species was P. subcapitata, for which the EC for CTC, OTC and ENF were 1.19 ± 0.53, 0.92 ± 0.30 and 5.18 ± 3.80 mg L, respectively. The EC for A. fusiformis, were 3.23 ± 0.53, 7.15 ± 2.69 and 10.6 ± 1.28 mg L, respectively. The genotoxicity of these veterinary antibiotics was also assessed using chromosome aberration (CA) and micronuclei (MN) induction in Allium cepa roots. Three concentrations were tested (0.1, 1 and 10 mg L). Only ENF at 1 and 10 mg L showed any significant MN induction. These data revealed that CTC, OTC and ENF could cause toxicity on green algae, whereas ENF could cause genotoxicity on A. cepa plants.

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Comparative genotoxicity of quinolone and quinolonyl-lactam antibacterials in the in vitro micronucleus assay in Chinese hamster ovary cells

Cited by 19 publications

References 2 publications

Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Aneugenic potential of okadaic acid revealed by the micronucleus assay combined with the FISH technique in CHO-K1 cells

Toxicity and Genotoxicity of Three Antimicrobials Commonly Used in Veterinary Medicine

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