Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test

Abstract

, et al.. Assessment of the genotoxicity of quinolone and fluoroquinolones contaminated soil with the Vicia faba micronucleus test. Ecotoxicology and Environmental Safety, Elsevier, 2012, 76, pp.187-192. <10.1016/j.ecoenv.2011 a b s t r a c tThe genotoxicity of quinolone and fluroquinolones was assessed using the micronucleus (MN) test on Vicia faba roots by direct contact exposure to a solid matrix. Plants were exposed to quinolones (nalidixic acid) and fluoroquinolones (ciprofloxacin and enrofloxacin) alone or mixed with artificially contaminated soils. Four different concentrations of each of these antibiotics were tested (0.01, 0.1, 1 and 10 mg/Kg) for nalidixic acid and (0.005, 0.05, 0.5 and 5 mg/Kg) for ciprofloxacin and enrofloxacin. These antibiotics were also used in mixture. Exposure of Vicia faba plants to each antibiotic at the highest two concentrations showed significant MN induction. The lowest two concentrations had no significant genotoxic effect. The mixture of the three compounds induced a significant MN induction whatever the mixture tested, from 0.02 to 20 mg/Kg. The results indicated that a similar genotoxic effect was obtained with the mixture at 0.2 mg/Kg in comparison with each molecule alone at 5-10 mg/Kg. Data revealed a clear synergism of these molecules on Vicia faba genotoxicity.

Toxicological Assessment of Trace β-Diketone Antibiotic Mixtures on Zebrafish (Danio rerio) by Proteomic Analysis

“…For example, they may cause DNA damage, adduct formation, DNA hypermethylation in kidney cells of Carassius auratus , and synchronous teratogenic effects on Cyprinus carpio [8]–[9]. To date, most previous research has focused on the toxicity and relative mechanist effects for single DKA compound [7]–[9]; few studies have addressed exposure to mixtures of DKA compounds. In real-world environments, the joint action of different pollutants may be very complex.…”

Joint toxicity of fluoroquinolone and tetracycline antibiotics to zebrafish (<i>Danio rerio</i>) based on biochemical biomarkers and histopathological observation

“…Tetracyclines facilitate a resultant fatty liver by affecting the gene expression associated with lipid metabolism for increasing triglyceride (TG) and cholesterol biosynthesis and decreasing β-oxidation of fatty acids (Yin et al, 2006). Previous studies showed that DKAs have strong genetic toxicity resulting in DNA damage, adduct formation, DNA hypermethylation of kidney cells in Carassius auratus and teratogenic effects on Cyprinus carpio (Khadra et al, 2012;Li et al, 2010). Among the toxicities of quinolones, one major risk is their chondrotoxicity potential in juvenile animals, such as rats, dogs, rabbits, nonhuman primates, and others (Sheng et al, 2013).…”

“…Previous reports mainly focused on toxicity and associated mechanisms for a single DKA compound (Khadra et al, 2012;Li et al, 2010;Wang et al, 2014). However, little information is available on the aquatic toxicity of mixed DKAs using zebrafish (Danio rerio) as a model organism.…”

Efficiency of co-composting process to remove genotoxicity from sewage sludge contaminated with hexavalent chromium

“…It has been demonstrated that raw sludge contains some compounds that are toxic and genotoxic for plants. Among them, organic acids, phenolic and pharmaceuticals compounds such as antibiotic (El Hajjouji et al, 2007;Khadra et al, 2012;El Fels et al, 2014a). These results underline the efficiency of cocomposting process to degrade and stabilise these compounds, and especially the Cr(VI).…”