Comparative Genome Analysis and Evaluation of Probiotic Characteristics of Lactobacillus plantarum Strain JDFM LP11

Heo, Jae-Young; Shin, Donghyun; Chang, Sung-Ho; Bogere, Paul; Park, Mi Ri; Ryu, Sangdon; Lee, Woong Ji; Yun, Bohyun; Lee, Hak Kyo; Kim, Young-Hoon; Oh, Sangnam

doi:10.5851/kosfa.2018.e21

Cited by 14 publications

(15 citation statements)

References 32 publications

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“…The L . plantarum strain JDFM LP11 used in this study possesses significant probiotic potential, with enhanced acid/bile tolerance, attachment to porcine intestinal epithelial cells (IPEC-J2), and antimicrobial activity [23]. Average daily intake of L .…”

Section: Resultsmentioning

confidence: 99%

“…Liquid probiotics used in this study containing L . plantarum strain JDFM LP11 (2.5×10 7 CFU/ml), 1% glucose, 1% molasses, 0.2% sea salt and 0.2% yeast extract (Eco Probiotics Solution, lot number: 061217, Doozy Probiotics Co., Ltd., Jeolabuk-do, South Korea) as described previously [23, 24] were stored at 4 °C and mixed into a weaner diet (FARMSCO Inc., Gyeonggi-do, South Korea) every morning for probiotic group (50 ml aliquots/kg of diet, 1.25×10 9 CFU/kg of diet). After 1 week allowing for adaptation, each piglet was fed a weaner diet or probiotic diet ad libitum with free access to fresh water during a 4 week trial.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we investigated beneficial roles of probiotics in gut microbiota modulation and immune response on gut heath in three-way crossbred (LYD) weaned piglets through analysis of fecal microbiome and intestinal transcriptome using L . plantarum strain JDFM LP11 possessing significant probiotic potential [23] and influencing on meat quality and chemical characteristics in longissimus muscles of slaughtered pigs [24].…”

Section: Introductionmentioning

confidence: 99%

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Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs

et al. 2019

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The importance of probiotics in swine production is widely acknowledged as crucial. However, gaps still remain in the exact roles played by probiotics in modulation of gut microbiota and immune response. This study determined the roles of probiotic Lactobacillus plantarum strain JDFM LP11in gut microbiota modulation and immune response in weaned piglets. L. plantarum JDFM LP11 increased the population of lactic acid bacteria in feces and enhanced the development of villi in the small intestine. Metagenome analysis showed that microbial diversity and richness (Simpson, Shannon, ACE, Chao1) and the relative abundance of the Firmicutes were higher in weaned piglets fed probiotics. Five bacterial families were different in the relative abundance, especially; Prevotellaceae occupied the largest part of microbial community showed the most difference between two groups. Transcriptome analysis identified 25 differentially expressed genes using RNAsequencing data of the ileum. Further gene ontology and immune DB analysis determined 8 genes associated with innate defense response and cytokine production. BPI, RSAD2, SLPI, LUM, OLFM4, DMBT1 and C6 genes were down-regulated by probiotic supplementation except PLA2G2A. PICRUSt analysis predicting functional profiling of microbial communities indicated branched amino acid biosynthesis and butyrate metabolism promoting gut development and health were increased by probiotics. Altogether, our data suggest that L. plantarum JDFM LP11 increases the diversity and richness in the microbial community, and attenuates the ileal immune gene expression towards gut inflammation, promoting intestinal development in weaned piglets.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs

et al. 2019

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“…Poor sanitary and hygienic situations occur when the pens are not cleaned and disinfected prior to placement and during the rearing period, because of negligent stockmanship and unremoved leftover feed, among others. (Heo et al, 2018;Mizumachi et al, 2009;Pieper et al, 2011;Suo et al, 2012) L. fermentum I5007 (Liu et al, 2014) L. casei GG (Roselli et al, 2005) L. acidophilus C3, NCDC-15 (Dowarah, Verma, et al, 2017a;Giang et al, 2011) L. brevis ATCC 8287, 1E1 (Gebert et al, 2011;Lähteinen et al, 2014) L (Brown et al, 1997;Estrada et al, 2001) B. animalis CSCC 1941, MB5 (Bird et al, 2009;Roselli et al, 2005 (Priori et al, 2015) GRAS: Generally regarded as safe, GIT: Gastrointestinal tract, LAB: Lactic acid bacteria Poor hygiene allows the proliferation of pathogenic microorganisms in the piglets' environment, which terminally find their way into the gut via the faecal-oral pathway. Post-weaning feeding and feeding regimes have been implicated in PWD, and the sudden change from sow milk to a solid ration, as occurs during weaning, has been demonstrated to reduce villus height while increasing crypt depths in the ileum, leading to diarrhoea (Dong & Pluske, 2007).…”

Section: Post-weaning Diarrhoea In Pigletsmentioning

confidence: 99%

Probiotics as alternatives to antibiotics in treating post-weaning diarrhoea in pigs: Review paper

Bogere¹,

Choi²,

Heo³

2019

SA J. An. Sci.

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The use of antibiotics to prevent post-weaning diarrhoea (PWD) in pigs has faced a setback owing to the associated antibiotic resistance in pigs and in the human populace that consumes the pork. In fact, antibiotic resistance that originates from the food chain is estimated to cause around 700,000 deaths globally each year. Consequently, scientists and researchers have suggested possible alternatives to antibiotics in pig diets. The chief of these has been the use of probiotics. The authors reviewed the literature on the use of probiotics as an alternative to antibiotics in treating PWD in pigs. It is clear that because of pathogenic Escherichia coli PWD continues to be a challenge to profitable swine production. The vast number of studies that was reviewed, point to the beneficial effects of probiotic supplementation on reducing the severity and incidence of PWD. However, some studies report inconsistencies to the general hypothesis. The majority of the microorganisms used as probiotics in the studies belong to the genera Lactobacilli, Bacillus, Bifidobacterium, Enterococcus, probiotic Escherichia coli, and Saccharomyces. The review also revealed that the bacterial strains that are used as probiotics are given individually or as combinations of multiple strains, and at various dosages, yielding varied results in each case. Interestingly, the authors observed wide disparities in the onset of probiotic supplementation and duration of the treatment to attain the results. Hence there is a need to standardize supplementation strategies, including dosage, onset and duration of treatment for probiotics. Furthermore, many of the in vivo studies that revealed positive effects of probiotics on diarrhoea and other production parameters were carried out in more controlled environments. The authors therefore suggest that more field studies in more natural and commercial farm settings should be conducted to augment the literature in relation to the use of probiotics as alternatives to antibiotics in treating PWD.

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“…Probiotic products were traditionally considered to exert proper health-promoting effects only when administered alive, but recent evidences are increasing that administration of the postbiotic molecules secreted from beneficial bacteria may be sufficient to promote the desired effects (Heo et al., 2018; Ruiz et al, 2014). Moreover, since administration of live bacteria strains always involves a risk of acting as a reservoir for antibiotic resistance genes which have potential to pass them to pathogenic bacteria and its stability and viability in host body is difficult to determine, utilization of probiotic bacterial extracellular molecules may represent a safer alternative way for the application of probiotic bacteria (Mehdi et al, 2016; Yan et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression and Purification of Extracellular Solute-Binding Protein (ESBP) in Escherichia coli, the Extracellular Protein Derived from Bifidobacterium longum KACC 91563

Song¹,

Kim²,

Park³

et al. 2019

Food Sci Anim Resour

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Bifidobacterium longum KACC 91563 secretes family 5 extracellular solute-binding protein via extracellular vesicle. In our previous work, it was demonstrated that the protein effectively alleviated food allergy symptoms via mast cell specific apoptosis, and it has revealed a therapeutic potential of this protein in allergy treatment. In the present study, we cloned the gene encoding extracellular solute-binding protein of the strain into the histidine-tagged pET-28a(+) vector and transformed the resulting plasmid into the Escherichia coli strain BL21 (DE3). The histidine-tagged extracellular solute-binding protein expressed in the transformed cells was purified using Ni-NTA affinity column. To enhance the efficiency of the protein purification, three parameters were optimized; the host bacterial strain, the culturing and induction temperature, and the purification protocol. After the process, two liters of transformed culture produced 7.15 mg of the recombinant proteins. This is the first study describing the production of extracellular solute-binding protein of probiotic bacteria. Establishment of large-scale production strategy for the protein will further contribute to the development of functional foods and potential alternative treatments for allergies.

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Comparative Genome Analysis and Evaluation of Probiotic Characteristics of Lactobacillus plantarum Strain JDFM LP11

Cited by 14 publications

References 32 publications

Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs

Beneficial roles of probiotics on the modulation of gut microbiota and immune response in pigs

Probiotics as alternatives to antibiotics in treating post-weaning diarrhoea in pigs: Review paper

Expression and Purification of Extracellular Solute-Binding Protein (ESBP) in Escherichia coli, the Extracellular Protein Derived from Bifidobacterium longum KACC 91563

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