Acne is a chronic inflammatory skin disease induced by Cutibacterium acnes. Recently, the effects of probiotics, prebiotics and synbiotics have been researched for the treatment of skin diseases in humans. However, the synbiotic effect of probiotics and prebiotic Curcuma longa rhizome extract (CLE) on C. acnes remains ambiguous. Therefore, the aim of this study was to investigate the synergistic antibacterial activities of probiotic lactic acid bacteria (LAB) with CLE as a synbiotic against C. acnes. Agar well diffusion assays were performed to determine the inhibitory effects of each combination of one of five Lactobacillus sp. with CLE as synbiotics against C. acnes KCTC 3314. Among them, the comparison between the average diameters of inhibition zones showed that the synbiotic combination of Lactobacillus acidophilus A001F8 and CLE significantly increased the inhibition zone diameters against C. acnes, compared to the use of Lactobacillus acidophilus A001F8 or CLE alone (p < 0.05). In conclusion, the synbiotics of probiotic LAB and CLE showed synergistic antibacterial effects against C. acnes, suggesting therapeutic potential for this synbiotic combination in the development of cosmetics or medicine against C. acnes.
Bifidobacterium longum
KACC 91563 secretes family 5
extracellular solute-binding protein via extracellular vesicle. In our previous
work, it was demonstrated that the protein effectively alleviated food allergy
symptoms via mast cell specific apoptosis, and it has revealed a therapeutic
potential of this protein in allergy treatment. In the present study, we cloned
the gene encoding extracellular solute-binding protein of the strain into the
histidine-tagged pET-28a(+) vector and transformed the resulting plasmid
into the
Escherichia coli
strain BL21 (DE3). The
histidine-tagged extracellular solute-binding protein expressed in the
transformed cells was purified using Ni-NTA affinity column. To enhance the
efficiency of the protein purification, three parameters were optimized; the
host bacterial strain, the culturing and induction temperature, and the
purification protocol. After the process, two liters of transformed culture
produced 7.15 mg of the recombinant proteins. This is the first study describing
the production of extracellular solute-binding protein of probiotic bacteria.
Establishment of large-scale production strategy for the protein will further
contribute to the development of functional foods and potential alternative
treatments for allergies.
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