Comparative genetic diversity studies of Theobroma cacao L. using RFLP and RAPD markers

N'Goran, Jeanne A.K.; Laurent, V.; Risterucci, Ange-Marie; Lanaud, Claire

doi:10.1038/hdy.1994.166

Cited by 75 publications

(38 citation statements)

References 12 publications

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“…This class also included material from germplasm collections in Costa Rica, Cô te d'Ivoire, Mexico, Venezuela and France. 'Modern Criollo' represents the genotypes studied as Criollo in previous biochemical and molecular studies (Lanaud, 1987;Bekele and Bekele, 1998;Ronning and Schnell, 1994;Laurent et al, 1995;N'Goran et al, 1994;De la Cruz et al, 1995;Lerceteau et al, 1997;Whitkus et al, 1998).…”

Section: Plant Materialsmentioning

confidence: 99%

Cacao domestication I: the origin of the cacao cultivated by the Mayas

et al. 2002

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Criollo cacao (Theobroma cacao ssp. cacao) was cultivated by the Mayas over 1500 years ago. It has been suggested that Criollo cacao originated in Central America and that it evolved independently from the cacao populations in the Amazon basin. Cacao populations from the Amazon basin are included in the second morphogeographic group: Forastero, and assigned to T. cacao ssp. sphaerocarpum. To gain further insight into the origin and genetic basis of Criollo cacao from Central America, RFLP and microsatellite analyses were performed on a sample that avoided mixing pure Criollo individuals with individuals classified as Criollo but which might have been introgressed with Forastero genes. We distinguished these two types of individuals as Ancient and Modern Criollo. In contrast to previous studies, Ancient Criollo individuals formerly classified as 'wild', were found to

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Section: Plant Materialsmentioning

confidence: 99%

Cacao domestication I: the origin of the cacao cultivated by the Mayas

et al. 2002

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“…Therefore, markers provide an efficient method for genetic resources characterization, through which genetic diversity and organization at different levels can be assessed (Karp & Edwards, 1997;Lanaud & Lebot, 1997). RFLP has been broadly and successfully used to characterize genetic resources including some tropical perennial crops (Besse et al, 1994;N'goran et al, 1994;Jack et al, 1995;Lebrun et al, 1995;Luo & Boutry, 1995;Lerceteau et al, 1997). However, this technique requires a relatively large quantity of good quality DNA and the best results are obtained using radioisotopes, which makes RFLP technically and ecologically sensitive.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers

Barcelos

Amblard

Berthaud

et al. 2002

Pesq. agropec. bras.

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-The objective of this work was to evaluate the genetic diversity, its organization and the genetic relationships within oil palm (Elaeis oleifera (Kunth) Cortés, from America, and E. guineensis (Jacq.), from Africa) germplasm using Restriction Fragment Length Polymorphism (RFLP) and Amplified Fragment Length Polymorphism (AFLP). In complement to a previous RFLP study on 241 E. oleifera accessions, 38 E. guineensis accessions were analyzed using the same 37 cDNA probes. These accessions covered a large part of the geographical distribution areas of these species in America and Africa. In addition, AFLP analysis was performed on a sub-set of 40 accessions of E. oleifera and 22 of E. guineensis using three pairs of enzyme/primer combinations. Data were subjected to Factorial Analysis of Correspondence (FAC) and cluster analysis, with parameters of genetic diversity being also studied. Results appeared congruent between RFLP and AFLP. In the E. oleifera, AFLP confirmed the strong structure of genetic diversity revealed by RFLP, according to geographical origin of the studied material, with the identification of the same four distinct genetic groups: Brazil, French Guyana/Surinam, Peru, north of Colombia/Central America. Both markers revealed that genetic divergence between the two species is of the same magnitude as that among provenances of E. oleifera. This finding is in discrepancy with the supposed early tertiary separation of the two species. Os resultados obtidos apresentaram grande coerência entre as duas técnicas utilizadas, RFLP e AFLP. Na espécie E. oleifera, a técnica AFLP confirmou a forte estruturação da diversidade genética revelada pela técnica de RFLP, de acordo com a origem geográfica do material estudado e com a identificação dos mesmos quatro grupos genéticos distintos: Brasil, Guiana Francesa/Suriname, Peru e norte da Colômbia/ América Central. Entretanto, ambos os marcadores revelaram que a divergência entre as duas espécies é da mesma magnitude da divergência dentro da espécie americana. Este resultado está em discrepância com uma suposta separação das duas espécies durante a era terciária.Termos para indexação: Elaeis oleifera, Elaeis guineensis, genética de população, palmeiras, recursos genéticos, caracterização molecular.

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“…In general, accessions from the Upper Amazon germplasm show higher GD than those from other regions (MARITA et al, 2001;RONNING et al, 1995;N'GORAN et al, 1994;LERCETEAU et al, 1997;WHITKUS et al, 1998). The clone set studied here consisted of Upper and Lower Amazon and Trinitario germplasm, comprising 20 TSA-644 descendants and 10 non-descendants.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data

et al. 2005

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Genetic diversity in cocoa (Theobroma cacao L.) has been assessed based on morphological and molecular markers for germplasm management and breeding purposes. Pedigree data is available in cocoa but it has not been used for assessing genetic relatedness. The geneitic diversity of 30 clonal cocoa accessions resistant to witche´ broom disease, from the CEPEC series, were studied on the basis of RAPD data and pedigree information. Twenty of these accessions descend from the TSA-644 clone, originated from a cross between the Upper Amazon germplasm called Scavina-6, the main source of resistance to witches' broom disease, and IMC-67. The ten remaining clones come from different sources including Amazon and Trinitario germplasm. RAPD data was collected using 16 primers and pedigree information was obtained from the International Cocoa Germplasm Database. Genetic similarities, genetic distances and coefficient of parentage were calculated using available software. Relatively low genetic diversity was observed in this germplasm set, probably because of great genetic relatedness amongst accessions studied and the poor representation of the germplasm. The TSA-644 descendants were more diverse than the other accessions used in the study. This might be due to the origin of the TSA clone, which was derived from highly divergent genotypes. Association between genetic similarities based on RAPD data and coefficient of parentage, based on pedigree data, was very low, probably due to the homogeneity of the breeding stocks and poor pedigree information. These findings are useful to cocoa breeders in planning crosses for the development of hybrid and clonal cultivars.

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Comparative genetic diversity studies of Theobroma cacao L. using RFLP and RAPD markers

Cited by 75 publications

References 12 publications

Cacao domestication I: the origin of the cacao cultivated by the Mayas

Cacao domestication I: the origin of the cacao cultivated by the Mayas

Genetic diversity and relationship in American and African oil palm as revealed by RFLP and AFLP molecular markers

Assessment of genetic diversity on a sample of cocoa accessions resistant to witches' broom disease based on RAPD and pedigree data

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