Comparative GC–FID and GC–MS analysis of the mono and sesquiterpene secondary metabolites produced by the field grown and micropropagated plants of Artemisia amygdalina Decne

Rather, Manzoor A.; Ganai, Bashir Ahmad; Kamili, Azra N.; Qayoom, Mubashir; Akbar, Seema; Akbar, Muhammad; Rasool, Roohi; Wani, Sajad H; Qurishi, Mushtaq A.

doi:10.1007/s11738-011-0886-6

Cited by 16 publications

(9 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secondary metabolites previously identified in micropropagated plant material was comparable to or was higher than that obtained from greenhousegrown, field-grown or wild-growing plants ( Arikat et al 2004;Guo et al 2007;Al-Qudah et al 2011;Ncube et al 2011;Rather et al 2012 (Murav'eva and Bubenchikova. 1986) , C. isaurica (Flamini et al 2004) and C. horrida (Flamini et al 2002).…”

Section: Observations Of Variable Rooting Frequencies Depended On Spementioning

confidence: 50%

Direct plant regeneration from different explants through micropropagation and determination of secondary metabolites in the critically endangered endemicRhaponticoides mykalea

Hayta

Bayraktar

Erel

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

View full text Add to dashboard Cite

Direct plant regeneration from different explants, micropropagation and determination of secondary metabolites were studied in the critically endangered endemic Rhaponticoides mykalea (Hub.-Mor.) M.V. Agab & Greuter. Seed germination was achieved by damaging the seed coat and cultivating the embryos on Woody Plant Medium (WPM), of which 40% germinated. The epicotyls and cotyledonary petioles of seedlings were used as initial explants and direct shoot regeneration was obtained on WPM containing 2.22 μM 6-benzyladenine (BA). WPM medium supplemented with 2.22 μM BA and 4.92 μM indole-3-butyric acid (IBA) significantly improved the production of multiple shoots, resulting in an average of 5.6 shoots per explants. The highest rooting of shoots (35.6%) was observed with WPM medium containing 19.68 μM IBA with 990 μM putrescine.Plantlets with well-developed roots were transferred to soil and acclimatised within a A c c e p t e d M a n u s c r i p t 2 plant growth chamber. Acclimatised plants showed 100% survival rate and remained healthy. As a part of our study, the content of secondary metabolites in three tissue culture regenerated lines were determined by HPLC analysis. Chlorogenic acid, Quercetin and scutellarin were confirmed secondary metabolites of R. mykalea.

show abstract

Section: Observations Of Variable Rooting Frequencies Depended On Spementioning

confidence: 50%

Direct plant regeneration from different explants through micropropagation and determination of secondary metabolites in the critically endangered endemicRhaponticoides mykalea

Hayta

Bayraktar

Erel

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

View full text Add to dashboard Cite

show abstract

“…The p-cymene (PC) [1-methyl-4-(1-methylethyl) benzene] is a monocyclic monoterpene, found in plants of the genus Protium, Thymus and Artemisia (Pinto et al, 2010;Rather et al, 2012;Salas et al, 2012). Medicinal plants such as oregano, a favorite in cuisine worldwide, are a rich source of essential oil with a significant amount of PC (Kulisic et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

The anti-hyperalgesic and anti-inflammatory profiles ofp-cymene: Evidence for the involvement of opioid system and cytokines

Santana¹,

Guimarães

Chaves³

et al. 2015

Pharmaceutical Biology

View full text Add to dashboard Cite

(2015) The anti-hyperalgesic and anti-inflammatory profiles of p-cymene: Evidence for the involvement of opioid system and cytokines, Pharmaceutical Biology, 53:11, 1583-1590, DOI: 10.3109/13880209.2014 To link to this article: https://doi.org/10. 3109/13880209.2014.993040 Published online: 05 May 2015.Submit your article to this journal Objective: The objective of this study was to evaluate the antinociceptive and anti-inflammatory profiles of p-cymene (PC), a monocyclic monoterpene, and its possible mechanisms of action. Materials and methods: Mice treated acutely with PC (25, 50, or 100 mg/kg, i.p.) were screened for carrageenan-induced hyperalgesia and the inflammatory components of its cascade (30-180 min), carrageenan-induced pleurisy (4 h), and tail-flick test (1-8 h). Also, we observed the PC effect on the generation of nitric oxide by macrophages and the activation of neurons in the periaqueductal gray (PAG) by immunofluorescence.Results: PC reduced (p50.001) the hyperalgesia induced by carrageenan, TNF-a, dopamine, and PGE 2 . PC decrease total leukocyte migration (100 mg/kg: p50.01), neutrophils (50 and 100 mg/kg: p50.05 and 0.001), and TNF-a (25, 50, and 100 mg/kg: p50.01, 0.05, and 0.001, respectively), besides reducing NO production (p50.05) in vitro. PC produced antinociceptive effect in tail-flick test (p50.05), which was antagonized by naloxone, naltrindole, nor-BNI, and CTOP, and increased (p50.001) the number of c-Fos-immunoreactive neurons in PAG. Discussion and conclusion: These results provide information about the anti-hyperalgesic and anti-inflammatory properties of PC suggesting a possible involvement of the opioid system and modulating some pro-inflammatory cytokines.

show abstract

“…Even if mass spectrometry is currently probably the most widely application used detection method, the flame ionization detector (FID) remains of interest because it is universal for hydrocarbon compounds (Buchmabauer et al 1997;Hasanloo et al 2008;Rather et al 2012). Other detectors may present some interest in plant analysis such as the thermo-ionic detector also called NPD because it specifically detects nitrogen-(i.e., alkaloids) and phosphorus-containing compounds.…”

Section: Generalitiesmentioning

confidence: 99%

The Modern Art of Identification of Natural Substances in Whole Plants

Chaimbault

2014

Recent Advances in Redox Active Plant and Microbial Products

View full text Add to dashboard Cite

Comparative GC–FID and GC–MS analysis of the mono and sesquiterpene secondary metabolites produced by the field grown and micropropagated plants of Artemisia amygdalina Decne

Cited by 16 publications

References 8 publications

Direct plant regeneration from different explants through micropropagation and determination of secondary metabolites in the critically endangered endemicRhaponticoides mykalea

Direct plant regeneration from different explants through micropropagation and determination of secondary metabolites in the critically endangered endemicRhaponticoides mykalea

The anti-hyperalgesic and anti-inflammatory profiles ofp-cymene: Evidence for the involvement of opioid system and cytokines

The Modern Art of Identification of Natural Substances in Whole Plants

Contact Info

Product

Resources

About