Comparative fingerprint analyses of extracts from the root bark of wild<b><i>Hippocratea excelsa</i>and “Cancerina” by high-performance liquid chromatography</b>

Araujo-León, Jesús Alfredo; Ciau, Durcy-Verenice Ruiz; Martinez, Tania-Isolina Coral; Ciau, Zulema-Osiris Cantillo

doi:10.1002/jssc.201401480

Cited by 9 publications

(4 citation statements)

References 28 publications

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“…HPLC-DAD analysis of PUM was carried out with the help of Agilent technology-1200 series, Germany, equipped with UV detector and reverse phase analytical column Zorbex plus RSC8 (Agilent U.S.A); particle size 5 μm; capacity 25 ml for separation. The mobile phase composed entirely of elution solvent A as acetonitrile (5%); methanol (10%); water (85%); acetic acid (1%) and elution solvent B as acetonitrile (40%); methanol (60%); acetic acid (1%) following gradient program [20]. The flow rate was maintained at 1 ml/min keeping injection volume at 1 ml.…”

Section: Hplc-dad Analysis Of Pummentioning

confidence: 99%

Pilea umbrosa ameliorate CCl4 induced hepatic injuries by regulating endoplasmic reticulum stress, pro-inflammatory and fibrosis genes in rat

Naz

Khan

Zai

et al. 2020

Environ Health Prev Med

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Background Pilea umbrosa (Urticaceae) is used by local communities (district Abbotabad) for liver disorders, as anticancer, in rheumatism and in skin disorders. Methods Methanol extract of P. umbrosa (PUM) was investigated for the presence of polyphenolic constituents by HPLC-DAD analysis. PUM (150 mg/kg and 300 mg/kg) was administered on alternate days for eight weeks in rats exposed with carbon tetrachloride (CCl4). Serum analysis was performed for liver function tests while in liver tissues level of antioxidant enzymes and biochemical markers were also studied. In addition, semi quantitative estimation of antioxidant genes, endoplasmic reticulum (ER) induced stress markers, pro-inflammatory cytokines and fibrosis related genes were carried out on liver tissues by RT-PCR analysis. Liver tissues were also studied for histopathological injuries. Results Level of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and glutathione (GSH) decreased (p < 0.05) whereas level of thiobarbituric acid reactive substance (TBARS), H2O2 and nitrite increased in liver tissues of CCl4 treated rat. Likewise increase in the level of serum markers; alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and total bilirubin was observed. Moreover, CCl4 caused many fold increase in expression of ER stress markers; glucose regulated protein (GRP-78), x-box binding protein1-total (XBP-1 t), x-box binding protein1-unspliced (XBP-1 u) and x-box binding protein1-spliced (XBP-1 s). The level of inflammatory mediators such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) was aggregated whereas suppressed the level of antioxidant enzymes; γ-glutamylcysteine ligase (GCLC), protein disulfide isomerase (PDI) and nuclear erythroid 2 p45-related factor 2 (Nrf-2). Additionally, level of fibrosis markers; transforming growth factor-β (TGF-β), Smad-3 and collagen type 1 (Col1-α) increased with CCl4 induced liver toxicity. Histopathological scrutiny depicted damaged liver cells, neutrophils infiltration and dilated sinusoids in CCl4 intoxicated rats. PUM was enriched with rutin, catechin, caffeic acid and apigenin as evidenced by HPLC analysis. Simultaneous administration of PUM and CCl4 in rats retrieved the normal expression of these markers and prevented hepatic injuries. Conclusion Collectively these results suggest that PUM constituted of strong antioxidant chemicals and could be a potential therapeutic agent for stress related liver disorders.

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Section: Hplc-dad Analysis Of Pummentioning

confidence: 99%

Pilea umbrosa ameliorate CCl4 induced hepatic injuries by regulating endoplasmic reticulum stress, pro-inflammatory and fibrosis genes in rat

Naz

Khan

Zai

et al. 2020

Environ Health Prev Med

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“…The chromatographic fingerprints of the herbal medicine samples are typically used in identification and authenticity [35][36], which only indicates the qualitative similarity in the presence and distribution of the chemical components. The difference in the quantity of the chemical components is not addressed by qualitative similarity analysis.…”

Section: Introductionmentioning

confidence: 99%

Linear Quantitative Profiling Method Fast Monitors Alkaloids of Sophora Flavescens That Was Verified by Tri-Marker Analyses

2016

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The present study demonstrated the use of the Linear Quantitative Profiling Method (LQPM) to evaluate the quality of Alkaloids of Sophora flavescens (ASF) based on chromatographic fingerprints in an accurate, economical and fast way. Both linear qualitative and quantitative similarities were calculated in order to monitor the consistency of the samples. The results indicate that the linear qualitative similarity (LQLS) is not sufficiently discriminating due to the predominant presence of three alkaloid compounds (matrine, sophoridine and oxymatrine) in the test samples; however, the linear quantitative similarity (LQTS) was shown to be able to obviously identify the samples based on the difference in the quantitative content of all the chemical components. In addition, the fingerprint analysis was also supported by the quantitative analysis of three marker compounds. The LQTS was found to be highly correlated to the contents of the marker compounds, indicating that quantitative analysis of the marker compounds may be substituted with the LQPM based on the chromatographic fingerprints for the purpose of quantifying all chemicals of a complex sample system. Furthermore, once reference fingerprint (RFP) developed from a standard preparation in an immediate detection way and the composition similarities calculated out, LQPM could employ the classical mathematical model to effectively quantify the multiple components of ASF samples without any chemical standard.

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“…Nossack and co-workers quantified pristimerin and tingenone (maitenin) in hydroalcoholic and aqueous extracts from the leaves and root bark of Maytenus aquifolium (“espinheira santa”) by HPLC-UV coupled with mass spectrometry (LC-MS) as a procedure for assessing the quality of this phytomedicine [40]. Moreover, a simple HPLC method was developed for the identification and comparison of quinonemethide triterpenes in wild Hippocratea excelsa and “cancerina”, a method useful for the control of this herb used in Mexican traditional medicine as an alternative cancer treatment [41]. In addition, Roca-Mézquita and co-workers performed quantitative analysis of pristimerin and tingenone in a dichloromethane extract of Elaeodendron trichotomum , revealing that this species contains both celastroloids, although, unexpectedly, pristimerin was present in very low concentration [29].…”

Section: Resultsmentioning

confidence: 99%

Development and Validation of an HPLC-PDA Method for Biologically Active Quinonemethide Triterpenoids Isolated from Maytenus chiapensis

et al. 2019

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Background: Quinonemethide triterpenoids, known as celastroloids, constitute a relatively small group of biologically active compounds restricted to the Celastraceae family and, therefore, they are chemotaxonomic markers for this family. Among this particular type of metabolite, pristimerin and tingenone are considered traditional medicines in Latin America. The aim of this study was the isolation of the most abundant celastroloids from the root bark of Maytenus chiapensis, and thereafter, to develop an analytical method to identify pristimerin and tingenone in the Celastraceae species. Methods: Pristimerin and tingenone were isolated from the n-hexane-Et2O extract of the root bark of M. chiapensis through chromatographic techniques, and were used as internal standards. Application of a validated RP HPLC-PDA method was developed for the simultaneous quantification of these two metabolites in three different extracts, n-hexane-Et2O, methanol, and water, to determine the best extractor solvent. Results: Concentration values showed great variation between the solvents used for extraction, with the n-hexane–Et2O extract being the richest in pristimerin and tingenone. Conclusions: M. chiapensis is a source of two biologically active quinonemethide triterpenoids. An analytical method was developed for the qualification and quantification of these two celastroloids in the root bark extracts of M. chiapensis. The validated method reported herein could be extended and be useful in analyzing Celastraceae species and real commercial samples.

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Comparative fingerprint analyses of extracts from the root bark of wildHippocratea excelsaand “Cancerina” by high-performance liquid chromatography

Cited by 9 publications

References 28 publications

Pilea umbrosa ameliorate CCl4 induced hepatic injuries by regulating endoplasmic reticulum stress, pro-inflammatory and fibrosis genes in rat

Pilea umbrosa ameliorate CCl4 induced hepatic injuries by regulating endoplasmic reticulum stress, pro-inflammatory and fibrosis genes in rat

Linear Quantitative Profiling Method Fast Monitors Alkaloids of Sophora Flavescens That Was Verified by Tri-Marker Analyses

Development and Validation of an HPLC-PDA Method for Biologically Active Quinonemethide Triterpenoids Isolated from Maytenus chiapensis

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