Linear Quantitative Profiling Method Fast Monitors Alkaloids of Sophora Flavescens That Was Verified by Tri-Marker Analyses

Hou, Zhifei; Sun, Guoxiang; Guo, Yong

doi:10.1371/journal.pone.0161146

Cited by 5 publications

(1 citation statement)

References 32 publications

(30 reference statements)

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“…is another quantitative parameter to describe the accuracy of linear model. The S l , P l , and α were combined as ALQFM that is the optimization of simple linear quantitative profiling method to assess the quality of TCM/HP S comprehensively based on eight grades listed in Supporting Information Table S1.

r = \frac{0false \sum_{i = 1}^{n} ((), x_{i} - \overset{x_{i}}{true}) ((), y_{i} - \overset{y_{i}}{true})}{\sqrt{{\sum_{i = 1}^{n} (x_{i} - \vec{x_{i}})}^{2}} \sqrt{{\sum_{i = 1}^{n} (y_{i} - \vec{y_{i}})}^{2}}}

\begin{matrix} left b & left = & left \frac{n \sum_{i = 1}^{n} x_{i} y_{i} - \sum_{i = 1}^{n} x_{i} \sum_{i = 1}^{n} y_{i}}{n \sum_{i = 1}^{n} y_{i}^{2} - \sum_{i = 1}^{n} y_{i}^{2}} \\ left \times \frac{m_{R}}{m_{j}} \approx \frac{\bar{x}}{\bar{y}} \times \frac{m_{R}}{m_{j}} \times 100 % \\ left = & left R \times \frac{m_{R}}{m_{j}} \times 100 % \end{matrix}

0true S_{l} = \frac{1}{2} ((), r + S)

…”

Section: Theory and Principlesmentioning

confidence: 99%

Novel strategy for quality consistency evaluation of Chinese medicine “YIQING” tablet that combines the simultaneous quantification and screening of ten bioactive constituents

Gong

Hong

Sun

et al. 2017

J of Separation Science

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The UV characteristics for different categories compounds in complex traditional Chinese medicines and herbal preparations usually vary. Thus, to achieve the integral analysis of multiwavelength fingerprint characteristics, we introduced a novel strategy of multiwavelength total fusion profiling. The simultaneous separation and quantification of multiple components by reversed-phase high-performance liquid chromatography coupled with diode array detection was developed in an effective, accurate, and reliable way. Furthermore, a 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging assay was set up to detect and screen the bioactivity of similar-structure constituents (aloe-emodin, rhein, emodin, chrysophanol, baicalein, wogonin, baicalin, wogonoside, berberine hydrochloride, and jatrorrhizine hydrochloride). Moreover, the high-performance liquid chromatography DPPH assay was developed to monitor the relationship between the biological activity and the spatial structure, the number of hydroxyl groups, the concentration of the analytes in samples. The result of qualitative classification for 15 batches of "YIQING" tablets using principle component analysis was consistent with the quantitative fingerprint assessment using the average linear quantitative fingerprint method. Therefore, chemometrics, multiwavelength total fusion profiling in conjunction with average linear quantitative fingerprint method and antioxidant activity can control the quality of traditional Chinese medicines/herbal preparations comprehensively and practically.

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