Comparative evidence support better antioxidant efficacy of mitochondrial-targeted (Mitoquinone) than cytosolic (Resveratrol) antioxidant in improving in-vitro sperm functions of cryopreserved buffalo (Bubalus bubalis) semen

Tiwari, Swati; Mohanty, T.K.; Bhakat, Mukesh; Kumar, Narendra; Baithalu, Rubina Kumari; Nath, Sapna; Yadav, Hanuman Prasad; Dewry, Raju Kumar

doi:10.1016/j.cryobiol.2021.04.007

Cited by 18 publications

(12 citation statements)

References 56 publications

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“…In the present study, total motility, progressive motility, VAP, and VSL of post-thawed sperm were significantly better in MT200 and MT200+Tr groups, and other CASA-derived motility characteristics were the highest with MT200+Tr in the extender. This is congruent with previous studies, which have demonstrated the extender containing the concentration of 200 nM in human [21] and buffalo [38], and 150 nM in ram [40] have better sperm motility after cryopreservation. MitoQ supports ATP synthesis to maintain sperm motility through the electrons exchange of the redox cycle in the mitochondrial electron transport chain [37].…”

Section: Discussionsupporting

confidence: 93%

“…In our study, the extenders with 200 nM of MitoQ presented better post-thawed sperm quality characteristics such as viability, acrosome and membrane integrity, MMP and DNA fragmentation, which is in agreement with previous studies [6,13,25,38,41]. This preserving sperm quality can be related to the ability of MitoQ to directly lowed ROS levels in mitochondria.…”

Section: Discussionsupporting

confidence: 92%

“…The addition of MitoQ at various nanomolar concentrations in extender lowered the production of ROS and lipid peroxidation and improved sperm quality in frozen-thawed semen of catfish [13], human [21], buffalo [6], and rooster [25]. In buffalo semen, MitoQ indicated more cryo-tolerance than Resveratrol as a cytosolic antioxidant [38]. Improvement in sperm quality and antioxidant enzymatic activities was observed after the addition of 150 nM MitoQ to extender in cryopreserved ram semen; however, sperm mitochondrial function was not evaluated in this study [40].…”

Section: Introductionmentioning

confidence: 99%

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Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Rezaei

Bahmani²,

Mafakheri³

et al. 2022

Preprint

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The mitochondria-targeted antioxidant MitoQ has been regarded as an effective antioxidant agent against cryo-induced oxidative cellular damage. This study aimed to evaluate the use of different doses of MitoQ combined with trehalose to minimize mitochondrial impairment and oxidative stress during sperm cryopreservation of Markhoz goat. For this, semen collection was performed every 5 days from 5 bucks (10 ejaculates per buck). The ejaculates were pooled and then diluted in eight different Tris-based extenders as follows: no additives (control), 20, 200, 2000 nM of MitoQ (MT20, MT200, MT 2000, respectively), 150 mM of trehalose (Tr), MT20+Tr, MT200+Tr, MT2000+Tr. Each semen sample was frozen using a standard protocol, and sperm function and antioxidative status were evaluated after thawing. Results indicated higher total and progressive motility, acrosome and membrane integrity, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and lower DNA fragmentation and malondialdehyde in MT200+Tr than for all other groups except MT200; meanwhile, MT200 was also greater in these parameters than in the control group (P < 0.05). Furthermore, MT200 and MT200+Tr showed higher (P < 0.05) percentages of live cryopreserved sperm with high membrane mitochondrial potential than other groups. However, abnormality percentage and catalase activity of frozen-thawed sperm were not affected by treatments (P > 0.05). To conclude, we have found that supplementation of 200 nM MitoQ alone or in combination with 150 mM trehalose to semen extender improves the quality of cryopreserved sperm in goats, which is associated with enhanced antioxidant enzymatic defense and mitochondrial activity and reduced DNA fragmentation.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Rezaei

Bahmani²,

Mafakheri³

et al. 2022

Preprint

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“…Fu et al (2019) , stated that MitoQ supplementation in ram semen improves sperm membrane integrity. Moreover, Tiwari et al (2021) , reported that MitoQ had beneficial effect on buffalo sperm membrane integrity.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the performance of targeted mitochondrial antioxidant mitoquinone and non-targeted antioxidant pentoxifylline in improving rooster sperm parameters during freezing and thawing

Nazari¹,

Kia²,

Mehdipour³

et al. 2022

Poultry Science

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“…MitoQ mesylate was obtained from MedChemExpress, it was dissolved in DMSO before the cellular experiments following the manufacturer's instructions, and the working concentrations of MitoQ were referenced from previously published literature. [24][25][26] PQ dichloride was bought from Sigma-Aldrich. Rabbit antibodies used for Western blot including anti-MFN1 and anti-MFN2 were purchased from Proteintech, and a rabbit anti-GAPDH antibody was bought from Abways.…”

Section: Experimental Drugs and Main Reagentsmentioning

confidence: 99%

Mitoquinone mitigates paraquat‐induced A549 lung epithelial cell injury by promoting MFN1/MFN2‐mediated mitochondrial fusion

Liu

Sun

Wang

et al. 2022

J Biochem & Molecular Tox

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Paraquat (PQ) poisoning often leads to severe lung injuries, in which the mitochondria damage plays a critical role. Mitoquinone (MitoQ), a newly designed mitochondria‐targeted antioxidant, has been proved for its benefit in mitochondria protection. However, the role of MitoQ in PQ‐induced lung injury remains unclear. Thus, this study was performed to investigate the effect of MitoQ on PQ‐induced lung injury and its underlying mechanisms. Our work showed that PQ caused the inhibition of A549 lung epithelial cell viability in a dose‐dependent manner, while MitoQ remarkably mitigated the PQ‐induced cell viability suppression. Besides this, PQ‐mediated apoptosis of A549 cells was significantly attenuated by MitoQ, as indicated by the TUNEL assay and mitochondria membrane potential assay. Moreover, the intracellular reactive oxygen species (ROS) production was also dramatically suppressed when cotreated MitoQ with PQ. This could be ascribed to enhanced mitochondrial fusion mediated by Mitofusin 1 (MFN1)/Mitofusin 2 (MFN2), because MitoQ preserved mitochondrial network integrity, as reflected by MitoTracker staining, and MitoQ also increased the expression of MFN1/MFN2 in A549 cells after PQ treatment. Our data suggested MitoQ mitigated PQ‐induced lung epithelial cell injury by promoting MFN1/MFN2‐mediated mitochondrial fusion, and MitoQ might be a potential candidate drug for the treatment of PQ‐induced lung injury.

show abstract

Comparative evidence support better antioxidant efficacy of mitochondrial-targeted (Mitoquinone) than cytosolic (Resveratrol) antioxidant in improving in-vitro sperm functions of cryopreserved buffalo (Bubalus bubalis) semen

Cited by 18 publications

References 56 publications

Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Protective effects of different doses of MitoQ separately and combined with trehalose on sperm function and antioxidative status of cryopreserved Markhoz goat semen

Comparison of the performance of targeted mitochondrial antioxidant mitoquinone and non-targeted antioxidant pentoxifylline in improving rooster sperm parameters during freezing and thawing

Mitoquinone mitigates paraquat‐induced A549 lung epithelial cell injury by promoting MFN1/MFN2‐mediated mitochondrial fusion

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