Comparative evaluation of wound healing potential of manuka and acacia honey in diabetic and nondiabetic rats

Abstract

A BSTRACT Background: Manuka honey has attracted the attention of the scientific community for its antimicrobial and antioxidant properties. The active compounds of manuka honey to which its myeloperoxidase activity inhibition is owed are methyl syringate (MSYR) and leptosin (a novel glycoside of MSYR). The non-peroxide antibacterial activity is attributed to glyoxal, 3-deoxyglucosulose, and methylglyoxal. These properties make it an inexpensive and effective topical treatment in wound management. This study has focused on the evaluation of the effect of manuka honey and acacia honey on wound healing in nondiabetic and streptozotocin-induced diabetic rats. Materials and Methods: This study was conducted on a total of 42 rats (six rats in each group) and respective drug/substance was topically applied once daily on the excision wound for 21 days. Induction of diabetes was carried out in rats in groups IV, V, VI, and VII only. Measurement of wound contraction was carried out on days 3, 6, 9, 12, 15, 18, and 21 after operation. Time taken for the complete epithelization was recorded along with a histopathological examination of the healed wound bed. Results: Topical application of manuka honey achieved ≥80% wound contraction on day 9 after operation in both the nondiabetic and diabetic group. Complete epithelization was achieved 2 days earlier than the normal epithelization time in the manuka group. Histopathological examination showed well-formed keratinized squamous epithelium with normal collagen tissue surrounding hair follicles. Conclusion: This study provides good outcome with respect to wound healing (especially in diabetic condition) when manuka honey was compared to acacia honey and standard treatment.

Honey in wound healing: An updated review

“…In a comparative study on topical application of MH and acacia honey in diabetic and normal rats, it was reported that MH achieved up to 80% wound contraction after nine days of treatment. In the MH-treated group, complete epithelialization was evident two days earlier than usual epithelialization [ 61 ].…”

Efficacy of Bee Honey on Wound Healing: Split Skin Graft with Hyper-granulation Tissue

“…It was found that honey is effective bactericidal agent at wide range against both organisms 18 . Topical application of manuka honey was found to be effective and achieved ≥ 80% wound contraction in both the nondiabetic and diabetic group 19 .…”

“…Honey was found to be highly effective on the tested organisms of MRSA isolates with MIC and MBC ranging from 9.38-37.5% v/v 22 . Croatian honey, prominently honeydew honey, has been reported for its the very effective antibacterial activity and its potential to become an important additive to therapeutic techniques available to a medical practitioner against resistant pathogens 23 . Honey film has a greater effectiveness to accelerate the healing of burns and incision wounds when compared to the negative control 24 .…”

Therapeutic Properties of Honey for the Management of Wounds; Is There a Role in the Armamentarium of Diabetic Foot Ulcer Treatment? Results From In vitro and In vivo Studies

“…93 In another study, researchers compared the healing effects of topical application of manuka and acacia honey in streptozotocin-induced diabetic and nondiabetic male Wistar rats. 94 Rats were treated with an alginate gel that served as a control treatment, with manuka honey, with acacia honey, or with silver sulfadiazine cream for 21 days. Topical application of manuka honey was associated with higher wound contraction percentage and faster complete epithelialization of the wounds in both the diabetic and nondiabetic groups of animals, while this was not the case for wounds treated with acacia honey.…”

“…Topical application of manuka honey was associated with higher wound contraction percentage and faster complete epithelialization of the wounds in both the diabetic and nondiabetic groups of animals, while this was not the case for wounds treated with acacia honey. 94 Malkoç et al 95 evaluated the anti-inflammatory, antioxidant, and wound-healing effects of topical application of mad honey in streptozotocin-induced diabetic male Wistar albino rats. Rats received once daily topically on the wound saline solution, terramycin, or mad honey for 19 days.…”