Comparative evaluation of the seegene seeplex RV15 and real‐time PCR for respiratory virus detection

Abstract

Respiratory virus infections contribute substantially both to hospitalizations of young children, and to morbidity in immunocompromised patients such as those with hematological malignancies. Their rapid and accurate diagnosis is essential to patient management. To evaluate the prospective utility of Seeplex® DPO technology in respiratory virus diagnosis, a panel of 99 respiratory samples positive by real‐time RT‐PCR for one or more viruses was assayed by the Seegene Seeplex® RV12 system. As well as being able to detect all 10 viruses in the real‐time RT‐PCR system with the exception of enteroviruses, RV12 can also distinguish between the two subgroups of RSV and detect two subgroups of coronaviruses. Seven of the nine viruses in common with the RT‐PCR were detected reliably by RV12. Eleven samples RT‐PCR‐positive for Metapneumovirus and five samples positive for influenza B were not detected by RV12. Seegene developed a second‐generation system, RV15, which not only allowed detection of three additional viruses, but also addressed the potential problems with RV12 specificity. To address these concerns, 84 respiratory samples positive for a range of viruses by real‐time PCR were assayed with RV15. The results of this evaluation improved significantly upon those seen with RV12. The high throughput capabilities and potential lower technical requirements afforded by the Seeplex® system may offer an alternative to real‐time RT‐PCR systems. J. Med. Virol. 83:1469–1475, 2011. © 2011 Wiley‐Liss, Inc.

Outbreak of Febrile Respiratory Illness Caused by Adenovirus at a South Korean Military Training Facility: Clinical and Radiological Characteristics of Adenovirus Pneumonia

“…Complementary DNA (cDNA) was synthesized using random hexamers. Each cDNA preparation was subjected to multiplex PCR using Seeplex RV12 (Seegene, Seoul, Korea), which uses novel dualpriming oligonucleotides as PCR primers (14).…”

Viral Infection in Patients with Severe Pneumonia Requiring Intensive Care Unit Admission

“…The performance of the respiratory virus multiplex RT-PCR kit used in this study has been evaluated in published studies (14,15) (see online supplement).…”

Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses

“…Numerous laboratory-developed and commercial multiplex PCR assays using different amplification platforms have been described for respiratory viruses and have been generally shown to be superior to traditional diagnostic methods, such as virus culture and antigen detection for sensitive and specific detection of respiratory viruses (Arens et al, 2010;Bibby et al, 2011;Brittain-Long et al, 2010;Caliendo, 2011;Gadsby et al, 2010;Kim et al, 2009;Lamson et al, 2006;Mahony et al, 2007;Raymond et al, 2009). The US Food and Drug Administration (FDA) has cleared recently two commercial assays, the xTAG ® RVP Fast (Luminex Molecular Diagnostics, Austin, TX) and FilmArray ® Respiratory Panels (Idaho Technology Inc., Salt Lake City, Utah) (Rand et al, 2011), for invitro diagnostic use for multiplex detection of respiratory viruses.…”