Comparative evaluation of the efficiency of poliovulation induction in donor cows using “FSH-SUPER” drug with various injection schemes

Abstract

This article describes the problems of traditional poliovulation induction scheme with repeated injections of follicle-stimulating hormone (FSH) to embryo donor cows. The purpose of this study was a comparative analysis of poliovulation induction results in donors received one or several FSH injections. Experimental animals (n = 256) in group I (n = 96) received injections of "FSH-super" drug eight times, every 12 hours for 4 days; in group II (n = 73) there was one injection of "FSH-super" combined with polyvinyl alcohol with a molecular weight of 15,000 Da; in group III (n = 87) there was one injection of "FSH-super" combined with polyethylene glycol with a molecular weight of 6,000 Da. The number of donor cows with positive response to hormonal treatment in groups I-III was similar: 94.8%, 95.9%, and 95.4%, respectively, what shows the effectiveness of one injection of long acting FSH. At the same time, group III (with polyethylene glycol as prolongator) was the leader in the effectiveness of induced poliovulationhere the highest ovarian poliovulatory reaction was obtained with the formation of 15.2 yellow bodies per one donor (in groups II and I-14.0 and 10.5, respectively). The number of obtained proper embryos in group III was higher by 9.6% than in group II, and by 15.6% than in group I, while the number of degenerated embryos in group III was 2.5% less than in group II and 15.0% less than in group I. In group III, there were the highest level of ovum fertilization rate (82.2%), the largest number of excellent-and good-quality embryos (optimal for cryopreservation)-89.9%, and also, of embryos at the stages of late morula, early blastocyst and expanded blastocyst (optimal for transplantation)-93.3%. The considered facts can be taken into account when developing protocols for one FSH injection in order to stimulate poliovulation in embryo donor cows.

Factors affecting embryo engraftment in recipient cows

“…Ïðèìåíåíèå òåõíîëîãèè íà äàííîì óðîâíå å¸ ðàçâèòèÿ ïîçâîëÿåò â íåñêîëüêî äåñÿòêîâ ðàç óñêîðèòü âîñïðîèçâîäñòâî êðóïíîãî ðîãàòîãî ñêîòà, ñàìêè êîòîðîãî, êàê èçâåñòíî, ìàëîïëîäíû è ñïîñîáíû ðîaeäàòü îäíîãî òåëåíêà â ãîä, à â òå÷åíèå âñåãî ïåðèîäà ðåïðîäóêòèâíîé aeèçíè -8 -10 òåëÿò [13]. Äëÿ ñðàâíåíèÿ, â ïðîöåññå ïðèìåíåíèÿ ðàññìàòðèâàåìîé òåõíîëîãèè çà îäíó ñåññèþ èíäóêöèè ïîëèîâóëÿöèè îò îäíîé êîðîâû-äîíîðà âîçìîaeíî ïîëó÷èòü 20 è áîëåå ýìáðèîíîâ [14,15], à, ïðèíèìàÿ âî âíèìàíèå, ÷òî â òå÷åíèå îäíîãî ãîäà ó êîðîâû-äîíîðà äàííóþ òåõíîëîãèþ ïðèìåíÿþò 4 -6 ðàç, ÷òî ðàñïðîñòðàíåíî â õîçÿéñòâàõ, çàíèìàþùèõñÿ ïîëó÷åíèåì ýìáðèîíîâ êðóïíîãî ðîãàòîãî ñêîòà [16][17][18], òî ïîêàçàòåëü ïîëó÷àåìûõ ýìáðèîíîâ çà ãîä ìîaeåò ñîñòàâëÿòü 80 -140 øò., ïðè ïåðåñàäêå êîòîðûõ ðåöèïèåíòàì, ñ ó÷åòîì âûøåïðèâåäåííûõ ïîêàçàòåëåé óðîâíÿ ïðèaeèâëÿåìîñòè (â ñðåäíåì, 50 %), çà îäèí ãîä ìîaeíî ïîëó÷èòü 40 -70 òåëÿò [13]. Îäíàêî, íåñìîòðÿ íà äàííûå óñïåõè, ââèäó âîçðàñòàþùåé ïîòðåáíîñòè â ïðèìåíåíèè òåõíîëîãèè ýìáðèîòðàíñôåðà, âîïðîñû äîñòèaeåíèÿ áîëåå âûñîêîãî óðîâíÿ ïðèaeèâëÿåìîñòè ïåðåñàaeåííûõ ýìáðèîíîâ è âûõîäà áîëüøåãî êîëè÷åñòâà òåëÿò îñòàþòñÿ àêòóàëüíûìè.…”

EMBRYO SURVIVAL TO ACCELERATE GENETIC PROGRESS IN DAIRY HERDS (review)

“…Количество эмбрионов in vivo, продуцируемых коровами-донорами, варьирует в широком диапазоне (41)(42)(43). Согласно международной практике, у 30 % доноров отсутствует ответная реакция яичников на экзогенные гонадотропины (44), 30 % доноров проявляют крайне низкую ответную реакцию яичников с числом овуляций 1-3, что соответствует естественному процессу овуляции, и только от одной трети доноров получают суперовуляторный ответ с числом овуляций от 5 до 12 (45)(46)(47).…”

ВЫЖИВАЕМОСТЬ ЭМБРИОНОВ И ПОВЫШЕНИЕ ТЕМПОВ ГЕНЕТИЧЕСКОГО ПРОГРЕССА В МОЛОЧНЫХ СТАДАХ (Обзор)

“…Количество эмбрионов in vivo, продуцируемых коровами-донорами, варьирует в широком диапазоне (41)(42)(43). Согласно международной практике, у 30 % доноров отсутствует ответная реакция яичников на экзогенные гонадотропины (44), 30 % доноров проявляют крайне низкую ответную реакцию яичников с числом овуляций 1-3, что соответствует естественному процессу овуляции, и только от одной трети доноров получают суперовуляторный ответ с числом овуляций от 5 до 12 (45)(46)(47).…”