Comparative Evaluation of The Efficacy of Aloe Vera Gel with Milk and Hank’s Balanced Salt Solution in Maintaining the Viability of PDL Cells in Avulsed Teeth

Abraham, Baren; Kumaran, Parvathy; Varma, Beena; Xavier, Arun Mamachan; Kumar, Suresh

doi:10.7860/jcdr/2019/37876.12700

Cited by 10 publications

(15 citation statements)

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“…The analysis of cell viability was similar across the different studies: After storage of the teeth, the PDL cells were harvested, stained with 0.4% (w/v) trypan blue, and counted under a light microscope with a hemocytometer. Viable cells have intact cell membranes and do not absorb the blue stain, while the dye readily penetrates into the cytoplasm of the non‐viable cells with damaged cell membranes 33,34 . This method is considered one of the most accurate analyses to detect the remaining viable cells 22 .…”

Section: Resultsmentioning

confidence: 99%

“…Two RCTs evaluating storage of 50 extracted teeth in aloe vera gel for 45 minutes found conflicting results. Sharma et al 52 demonstrated significantly greater cell viability rates from storage of extracted teeth in aloe vera gel compared with storage in milk, while Abraham et al 33 found lower cell viability rates following storage of extracted teeth in aloe vera gel compared with milk (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…The extracted teeth in the studies were standardized since teeth with caries, periodontal diseases, and restorations were mostly excluded prior to randomization. It is of note that Abraham et al, 33 Martin and Pileggi, 43 and Rajendran et al 42 highlighted that the tooth extraction procedure may have induced variable trauma potentially leading to variability of PDL cell viability counts. Although the people performing the interventions were probably aware of the assigned intervention, no deviations from the intended interventions were expected that were likely to have affected the outcome at this stage.…”

Section: Resultsmentioning

confidence: 99%

“…Martin and Pileggi 43 reported that the cell viability was independently assessed by two researchers to minimize the risk of bias due to human errors. Finally, it was judged that studies reporting on cell growth rate or on number of viable PDL cells likely selected the outcome results from multiple measurements or data analyses, respectively 33,34,36,42,43,47,50,53 . In the outcome domain, percentage of viable PDL cells is clearly the most reported measurement and it is not appropriate to only show the number of viable PDL cells since it is not corrected for the total amount of cells in the sample.…”

Section: Resultsmentioning

confidence: 99%

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Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Brier

Dorien

Borra³

et al. 2020

Dental Traumatology

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Background/Aim It is crucial to store an avulsed tooth appropriately to preserve the viability of the periodontal ligament cells prior to replantation. The aim of this systematic review was to identify the best available evidence for the effectiveness of any technique available to laypeople for storing an avulsed tooth compared with storage in milk or saliva. Methods The following databases were searched (September 2019): Cochrane Library, MEDLINE, and Embase. Two reviewers independently considered trial eligibility, then extracted and analyzed data, and assessed the risk of bias. The certainty of the body of evidence was appraised according to the GRADE methodology. Results Out of 4118 references, 33 studies were included and reported 23 comparisons of which 10 were synthesized in a meta‐analysis. The limited evidence available favors storing an avulsed tooth in Hank's balanced salt solution (pooled SMD 2.47, 95% CI [1.59;3.34], P < .00001), propolis solution (pooled SMD 1.73, 95% CI [1.12;2.33], P < .00001), oral rehydration salts (pooled SMD 4.16, 95% CI [2.10;6.23], P < .0001), rice water, and cling film compared with storage in milk. The cell viability rate was significantly lower in teeth stored in saline solutions (pooled SMD −4.35, 95% CI [−7.55;−1.14], P = .008), tap water, buttermilk, castor oil, GC Tooth Mousse, and turmeric extract than those stored in milk. There is insufficient evidence to recommend for or against temporary storage of an avulsed tooth in saliva compared with alternative solutions. The certainty of evidence was considered low to very low due to limitations in study design, indirect study populations and outcome measures, and imprecision. Conclusion Although milk was shown to extend the periodontal ligament cell viability before replantation compared with saline or tap water, the following media have also demonstrated efficacy at preserving the cell viability: Hank's balanced salt solution, propolis, oral rehydration salts, rice water, and cling film.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Brier

Dorien

Borra³

et al. 2020

Dental Traumatology

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“…The First Aid Task Force requested a 2020 SysRev of media used to store an avulsed tooth until it can be reimplanted. 111 Many RCTs found benefit from immersion of the tooth in Hanks’ Balanced Salt Solution 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 , 156 , 157 as well as in oral rehydration salt solutions 154 , 155 or from wrapping the tooth in cling film (ie, plastic wrap) 158 as compared with immersion in milk. However, milk was better than many other media for storing a tooth until reimplantation.…”

Section: First Aidmentioning

confidence: 99%

Executive Summary 2020 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations

Nolan¹,

Maconochie²,

Soar³

et al. 2020

Resuscitation

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requiring first aid, related education and implementation strategies, and systems of care. After the publication of the 2015 International Consensus on CPR and ECC Science With Treatment Recommendations, ILCOR also committed to sponsoring a continuous evidenceevaluation process, with topics prioritized for review by the task forces and with CoSTR updates published annually. For this 2020 CoSTR, the 6 ILCOR task forces performed structured reviews of 184 topics, completing the most ambitious evidence review that ILCOR has attempted to date.The ILCOR systematic review process continues to be based on the methodological principles published by the National Academy of Health and Medicine (formerly the Institute of Medicine) 2 ; Cochrane 3,4 ; Grading of Recommendations Assessment, Development, and Evaluation (GRADE) 5 ; and the reporting guidelines based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses recommendations. 6,7 Three types of evidence evaluation provided the basis for this 2020 CoSTR: the systematic review, the scoping review, and the evidence update. Based on recommendations from the ILCOR Scientific Affairs Committee and agreement of the task forces, only systematic reviews could result in new or modified treatment recommendations.

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2020 International Consensus on First Aid Science With Treatment Recommendations

Singletary¹,

Zideman²,

Bendall³

et al. 2020

Resuscitation

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This is the summary publication of the International Liaison Committee on Resuscitation's 2020 International Consensus on First Aid Science WithTreatment Recommendations. It addresses the most recent published evidence reviewed by the First Aid Task Force science experts. This summary addresses the topics of first aid methods of glucose administration for hypoglycemia; techniques for cooling of exertional hyperthermia and heatstroke; recognition of acute stroke; the use of supplementary oxygen in acute stroke; early or first aid use of aspirin for chest pain; control of life-threatening bleeding through the use of tourniquets, hemostatic dressings, direct pressure, or pressure devices; the use of a compression wrap for closed extremity joint injuries; and temporary storage of an avulsed tooth. Additional summaries of scoping reviews are presented for the use of a recovery position, recognition of a concussion, and 6 other first aid topics. The First Aid Task Force has assessed, discussed, and debated the certainty of evidence on the basis of Grading of Recommendations, Assessment, Development, and Evaluation criteria and present their consensus treatment recommendations with evidence-to-decision highlights and identified priority knowledge gaps for future research.The 2020 International Consensus on Cardiopulmonary Resuscitation (CPR) and Emergency Cardiovascular Care (ECC) Science With Treatment Recommendations (CoSTR) is the fourth in a series of annual summary publications from the International Liaison Committee on Resuscitation (ILCOR). This 2020 CoSTR for first aid includes new topics addressed by systematic reviews performed within the past 12 months. It also includes updates of the first aid treatment recommendations published from 2010 through 2019 that are based on additional evidence evaluations and updates.As a result, this 2020 CoSTR for first aid represents the most comprehensive update since 2010.

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Comparative Evaluation of The Efficacy of Aloe Vera Gel with Milk and Hank’s Balanced Salt Solution in Maintaining the Viability of PDL Cells in Avulsed Teeth

Cited by 10 publications

References 0 publications

Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Storage of an avulsed tooth prior to replantation: A systematic review and meta‐analysis

Executive Summary 2020 International Consensus on Cardiopulmonary Resuscitation and Emergency Cardiovascular Care Science With Treatment Recommendations

2020 International Consensus on First Aid Science With Treatment Recommendations

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