Comparative evaluation of the DPP® CVL rapid test for canine serodiagnosis in area of visceral leishmaniasis

Laurenti, Márcia Dalastra; Leandro, M. V. de Santana; Tomokane, Thaíse Yumie; Lucca, H. R. L. De; Aschar, Mariana; Souza, C. S. F.; Silva, R. M.; Marcondes, Mary; Matta, Vânia Lúcia Ribeiro da

doi:10.1016/j.vetpar.2014.09.002

Cited by 70 publications

(88 citation statements)

References 22 publications

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“…After antigens were obtained, the ELISA technique was performed for each of the antigens as previously described 12 . Reference serum samples from the serum bank of the Laboratory of Parasitic Diseases in the Department of Preventive Veterinary Medicine and Animal Health at the College of Veterinary Medicine (FMVZ), University of São Paulo (USP), were included as negative and positive controls.…”

Section: Enzyme-linked Immunosorbent Assay With Crude Antigenmentioning

confidence: 99%

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Lima

Gennari

Soares

et al. 2017

Rev. Soc. Bras. Med. Trop.

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Section: Enzyme-linked Immunosorbent Assay With Crude Antigenmentioning

confidence: 99%

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Lima

Gennari

Soares

et al. 2017

Rev. Soc. Bras. Med. Trop.

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“…Moreover, the specificity of both TR DPP ® and ELISA can be low 15 , thereby compromising the efficacy of these screening methods in Brazil. Additionally, several conventional serological tests are limited due to their cross reactivity with other parasitic diseases 16 . Therefore, more efficient tests must be identified and combined with others for the improved identification of naturally infected animals 6 .…”

Section: Introductionmentioning

confidence: 99%

The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease

Mendonça

Batista

Schallig

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Dogs are considered the main reservoir of Leishmania infantum. This protozoan causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil. Serological tests and polymerase chain reaction (PCR) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (Teresina and Vitória). One-hundred infected and 57 non-infected animals from Teresina and 100 non-infected animals from Vitória were studied. Animal selection was not dependent on previous serology. The sensitivity (Teresina) and specificity (Teresina and Vitória) were as follows: indirect antibody fluorescence (IFAT) cut-off of 1:40 (IFAT 1:40): 96%, 18%, and 76%; IFAT 1:80: 90%, 33%, and 93%; direct agglutination test (DAT): 96%, 33%, and 98%; fast agglutination screening test (FAST): 93%, 68%, and 100%; immunochromatographic assay with a recombinant rK39 antigen (rK39): 88%, 74%, and 98%; enzyme linked immunosorbent assay (ELISA): 91%, 79%, and 98%; rapid dual-path platform test (TR DPP®): 98%, 60%, and 98%; and blood PCR: 29%, 93%, and 97%, respectively. In the high transmission area, none of the tests adequately discriminated L. infantum-infected from non-infected dogs. However, in the high transmission city, the area under the receiver operating characteristic (ROC) curve of FAST, DAT, ICrK39, ELISA and TR DPP® was high.

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“…Dogs that did not show clinical signs were considered subclinically infected dogs (SI group). The control group (CLT) consisted of dogs from the same areas of endemicity that had no clinical signs and tested negative by PCR, LST, LPA, and anti-L. infantum IgG enzyme-linked immunosorbent assay (ELISA) as described by Laurenti et al (37). Given the predicted effects of vaccination on the immune response, dogs that had previous vaccination or immunotherapy against CanL were removed from the CMI profile evaluation.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Study of Cell-Mediated Response in Dogs Naturally Infected by Leishmania infantum

et al. 2016

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A genome-wide association study (GWAS) could unravel the complexity of the cell-mediated immunity (CMI) to canine leishmaniasis (CanL). Therefore, we scanned 110,165 single-nucleotide polymorphisms (SNPs), aiming to identify chromosomal regions associated with the leishmanin skin test (LST), lymphocyte proliferation assay (LPA), and cytokine responses to further understand the role played by CMI in the outcome of natural Leishmania infantum infection in 189 dogs. Based on LST and LPA, four CMI profiles were identified (LST ؊ /LPA ؊ , LST ؉ /LPA ؊ , LST ؊ /LPA ؉ , and LST ؉ /LPA ؉ ), which were not associated with subclinically infected or diseased dogs. LST ؉ /LPA ؉ dogs showed increased interferon gamma (IFN-␥) and tumor necrosis factor alpha (TNF-␣) levels and mild parasitism in the lymph nodes, whereas LST ؊ /LPA ؉ dogs, in spite of increased IFN-␥, also showed increased interleukin-10 (IL-10) and transforming growth factor ␤ (TGF-␤) levels and the highest parasite load in lymph nodes. Low T cell proliferation under low parasite load suggested that L. infantum was not able to induce effective CMI in the early stage of infection. Altogether, genetic markers explained 87%, 16%, 15%, 11%, 0%, and 0% of phenotypic variance in TNF-␣, TGF-␤, LST, IL-10, IFN-␥, and LPA, respectively. GWAS showed that regions associated with TNF-␣ include the following genes: IL12RB1, JAK3, CCRL2, CCR2, CCR3, and CXCR6, involved in cytokine and chemokine signaling; regions associated with LST, including COMMD5 and SHARPIN, involved in regulation of NF-B signaling; and regions associated with IL-10, including LTBP1 and RASGRP3, involved in T regulatory lymphocytes differentiation. These findings pinpoint chromosomic regions related to the cell-mediated response that potentially affect the clinical complexity and the parasite replication in canine L. infantum infection.

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Comparative evaluation of the DPP® CVL rapid test for canine serodiagnosis in area of visceral leishmaniasis

Cited by 70 publications

References 22 publications

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

Serodiagnosis of visceral and cutaneous leishmaniasis in human and canine populations living in Indigenous Reserves in the Brazilian Amazon Region

The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease

Genome-Wide Association Study of Cell-Mediated Response in Dogs Naturally Infected by Leishmania infantum

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