2017
DOI: 10.1590/s1678-9946201759039
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The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease

Abstract: Dogs are considered the main reservoir of Leishmania infantum. This protozoan causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil. Serological tests and polymerase chain reaction (PCR) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (Teresina and Vitória). One-hundred infected and 57 non-infected animals from Teresina and 100 non-infected animals from Vitória were studied. Animal selection was not dependent on prev… Show more

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Cited by 26 publications
(22 citation statements)
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References 37 publications
(36 reference statements)
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“…These tests are antibody-based and do not effectively distinguish infected dogs from healthy dogs with an immunological memory of a previous infection, as we have previously shown 16,18 . Therefore, a large number of seropositive, not infective dogs have been culled in Brazil.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…These tests are antibody-based and do not effectively distinguish infected dogs from healthy dogs with an immunological memory of a previous infection, as we have previously shown 16,18 . Therefore, a large number of seropositive, not infective dogs have been culled in Brazil.…”
Section: Discussionmentioning
confidence: 99%
“…As previously reported 16 , we performed an enzyme-linked immunosorbent assay (ELISA), immunofluorescence antibody tests in 1:40 and 1:80 dilutions (IFAT40 and IFAT80), rapid dual path platform tests (RT-DPP), immunochromatographic assay with a recombinant rK39 antigen (ICrK39), fast agglutination screening tests (FAST), and direct agglutination tests (DAT), and polymerase chain reaction (PCR).…”
Section: Diagnostic Testsmentioning
confidence: 99%
“… 37 Another more recent study used the direct parasitological test associated with the parasite culture, and it estimated sensitivity for TR-DPP of 100%; its specificity varied between 22 and 96%. 38 The study by Silva et al 39 used serological tests as a reference standard and the TR-DPP’s sensitivity was 58.33% (CI95% 43 to 72). Carvalho et al 10 demonstrated that by using qPCR in blood serum samples, the protocol for CVL diagnosis prescribed by the Ministry of Health had a sensitivity of 67.24% (CI95% 54.42 to 77.92) and specificity of 86.59% (CI95% 77.55 to 92.34).…”
Section: Discussionmentioning
confidence: 99%
“…In addition, the three observers clearly verified the appearance of a blue‐coloured solution in only the positive samples and a pink‐coloured solution in the negative samples, without conflicting results. The highest specificity of pELISA is an important parameter because an incorrect identification may result in inadequate treatment or unnecessary euthanasia . The pELISA also presented the best PLR and NLR values (1.6 × 10 10 and .05, respectively).…”
Section: Discussionmentioning
confidence: 99%