Comparative evaluation of RapID ANA and API 20A for identification of anaerobic bacteria

Summanen, Paula; Jousimies‐Somer, Hannele

doi:10.1007/bf01975045

Cited by 32 publications

(16 citation statements)

References 12 publications

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“…Gram-positive facultative cocci-forming chains in the subcultures were classi®ed as streptococci and were identi®ed with the API 20 STREP system (API System, Vercieu, France). Anaerobic isolates were identi®ed using the pattern of metabolic end products and reactions in the RapID ANA II micromethod kit system (Innovative Diagnostic System Inc., Atlanta, GA, USA) (13). Additional enzymatic activities were tested with the aid of API ZYM (API System, Vercieu, France) (14,15).…”

Section: Microbiological Techniquesmentioning

confidence: 99%

Profile of subgingival microbiota in children with primary dentition

Kamma

Diamanti-Kipioti

Nakou

et al. 2000

J of Periodontal Research

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Eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. The aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4-5 yr, chosen randomly. Subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). The samples were cultured for bacterial isolation anaerobically and in 10% CO2 plus air using selective and non-selective media. Forty-one different microbial species were isolated. Gemella morbillorum and Peptostreptococcus magnus were statistically significantly more frequently detected in incisors while P. micros, Streptococcus intermedius, Bacteroides forsythus, Fusobacterium nucleatum, Prevotella loeschei, P. melaninogenica and Selenomonas sputigena were more frequently detected in molars. The bacterial species S. constellatus, G. morbillorum and P. magnus were isolated in greater numbers in incisors and P. micros, S. intermedius, Campylobacter concisus, Bacteroides egertheii, B. forsythus, P. oralis and S. sputigena were isolated in greater numbers in molars, respectively. Cluster analysis revealed 4 clusters in which 6-7 bacterial species were elevated above mean levels. Cluster I was predominated by S. constellatus, S. mitis, S. sanguis, G. morbillorum, P. melaninogenica and P. oralis; cluster II was predominated by S. sanguis, Actinomyces naeslundii, Capnocytophaga gingivalis, C. ochracea and P. intermedia; cluster III was predominated by S. mitis, C. ochracea, F. nucleatum, P. loeschei, P. melaninogenica and P. oralis; and finally cluster IV was predominated by S. sanguis, C. gingivalis, Veillonella parvula, Campylobacter gracilis, F. nucleatum and P. intermedia. The bacterial species S. constellatus, P. micros, Pseudoramibacter alactolyticus, Eikenella corrodens and F. nucleatum were associated with non-bleeding sites while S. intermedius, C. concisus, P. intermedia and P. loescheii were found more frequently in bleeding sites.

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Section: Microbiological Techniquesmentioning

confidence: 99%

Profile of subgingival microbiota in children with primary dentition

Kamma

Diamanti-Kipioti

Nakou

et al. 2000

J of Periodontal Research

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“…Isolates were further identified by determining their biochemical characteristics and carbohydrate utilisation by the prereduced anaerobically sterilised (PRAS) biochemicals (Holdeman and others 1977, others 1987, Jousimies-Somer andFinegold 1991). Profiles of preformed enzymes were determined by the Rap ID Ana I1 system (Innovative Diagnostic Systems, USA) (Summanen and Jousimies-Somer 1988) and by individual tablet substrates (Rosco; Taastrup, Denmark). Short chain fatty acid profiles of metabolic end products of glucose were determined by gas-liquid chromatography (Holdeman andothers 1977, Sutter andothers 1985).…”

Section: Microbiological Examin a Tionmentioning

confidence: 99%

Clinical, radiological and bacteriological findings in canine periodontitis

Sarkiala

Asikainen

Wolf

et al. 1993

J of Small Animal Practice

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The clinical and radiological features and bacterial flora were studied in 16 small dogs with periodontitis. Gingival retraction, bleeding and alveolar bone loss were the most typical findings, whereas deep periodontal pockets were infrequently found. Periodontitis was frequently localised to certain regions of the dentition, most often in premolars or incisors. However, the deepest periodontal pockets were found in canine teeth. The mean pocket depth was 2·0 ± 0·4 mm (mean ± SD). The mean percentage of the sites with a pocket depth of more than 3 mm was 10·5 per cent. The mean occurrence of gingival bleeding after probing was 22·7 ± 12·7 per cent and the mean percentage of furcation lesions in multirooted teeth per dog was 46·0 ± 23·5 per cent. Tooth mobility was seen in 26·7 ± 13·3 per cent of the teeth. In each case subgingival plaque samples were taken for microbiological examination from two teeth with periodontitis and one healthy tooth. There was a clear difference between the diseased and healthy pockets in the detection frequency of the following Gram‐negative anaerobes: pigmented, non‐pigmented slime producing and fusiform rods. The counts of Gram‐negative pigmented, other non‐pigmented and fusiform rods as well as Gram‐positive cocci were clearly higher in the diseased pockets. Pigmented Gram‐negative rods (mainly asaccharolytic, Porphyromonas‐like species) were the most common finding in both diseased and healthy pockets.

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“…It is now well established that anaerobes may be involved in numerous infections, including severe infections (8,12). Until recently, however, identification of anaerobes in clinical laboratories relied mainly on the use of time-consuming and labor-intensive conventional methods or of manual commercial systems, the performances of which are quite variable at the species level (3,5,7,10,13,14).…”

mentioning

confidence: 99%

Evaluation of the New Vitek 2 ANC Card for Identification of Medically Relevant Anaerobic Bacteria

et al. 2009

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Of 261 anaerobic clinical isolates tested with the new Vitek 2 ANC card, 257 (98.5%) were correctly identified at the genus level. Among the 251 strains for which identification at the species level is possible with regard to the ANC database, 217 (86.5%) were correctly identified at the species level. Two strains (0.8%) were not identified, and eight were misidentified (3.1%). Of the 21 strains (8.1%) with low-level discrimination results, 14 were correctly identified at the species level by using the recommended additional tests. This system is a satisfactory new automated tool for the rapid identification of most anaerobic bacteria isolated in clinical laboratories.

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Comparative evaluation of RapID ANA and API 20A for identification of anaerobic bacteria

Cited by 32 publications

References 12 publications

Profile of subgingival microbiota in children with primary dentition

Profile of subgingival microbiota in children with primary dentition

Clinical, radiological and bacteriological findings in canine periodontitis

Evaluation of the New Vitek 2 ANC Card for Identification of Medically Relevant Anaerobic Bacteria

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