Comparative Evaluation of Polyphenol Contents and Antioxidant Activities between Ethanol Extracts of Vitex negundo and Vitex trifolia L. Leaves by Different Methods

Abstract

The in vitro antioxidant potential assay between ethanolic extracts of two species from the genus Vitex (Vitex negundo L. and Vitex trifolia L.) belonging to the Lamiaceae family were evaluated. The antioxidant properties of different extracts prepared from both plant species were evaluated by different methods. DPPH scavenging, nitric oxide scavenging, and β-carotene-linoleic acid and ferrous ion chelation methods were applied. The antioxidant activities of these two species were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and Ethylene diamine tetra acetic acid (EDTA). Both species of Vitex showed significant antioxidant activity in all of the tested methods. As compared to V. trifolia L. (60.87–89.99%; 40.0–226.7 μg/mL), V. negundo has been found to hold higher antioxidant activity (62.6–94.22%; IC50 = 23.5–208.3 μg/mL) in all assays. In accordance with antioxidant activity, total polyphenol contents in V. negundo possessed greater phenolic (89.71 mg GAE/g dry weight of extract) and flavonoid content (63.11 mg QE/g dry weight of extract) as compared to that of V. trifolia (77.20 mg GAE/g and 57.41 mg QE/g dry weight of extract respectively). Our study revealed the significant correlation between the antioxidant activity and total phenolic and flavonoid contents of both plant species.

Nutritional Composition, Total Phenolic Content, Antioxidant and α-Amylase Inhibitory Activities of Different Fractions of Selected Wild Edible Plants

“…To our knowledge, there are little or no available data in the literature about the TPC and TFC of the selected plants; thus, only a few papers could be found related to the TPC and TFC of other species of the same families. The leaf extract of Vitex negundo has recently been reported to contain 89.71 mg GAE/g of TPC and 63.11 mg QE/g of TFC [30], which are relatively higher values than our study. Shahat et al [31] reported TPC in the range from 11 to 56 mg GAE/g of DW for different species of Asteraceae family, which is a lower value than our findings.…”

Evaluation of antioxidant, antibacterial and wound healing activities of Vitex pinnata

“…Closely related plant species have been shown to have similar phytochemical constituents ( Diris et al, 2017 ). The antioxidant activities of V. pinnata ’s close relatives, V. agnus-cactus , V. negundo and V. trifolia have also been determined using various solvents, and was found that the fruits and leaves of these Vitex plants were significantly capable of scavenging free radicals ( Rashed, 2013 ; Saklani et al, 2017 ), signifying that Vitex plants are potential sources of promising antioxidants.…”

Vitex Negundo Extracts Protect Human Corneal Epithelial Cells From Ultraviolet C-Induced Phototoxicity

“…The total phenolic content was high in the ethanol extract than the aqueous extract, and this property is attributed to their antioxidant activity. This is mainly due to the ability of the extracts to absorb and neutralize the free radicals, to quench the singlet, or triplet oxygen species [28]. The total flavonoid content was also found to be high in ethanol extract than the aqueous extract.…”