Comparative evaluation of microbial and chemical methods for assessing 4-chlorophenol biodegradation in soil

Nagy, Zsuzsanna Magdolna; Gruiz, Katalin; Molnár, Mónika; Fenyvesi, Éva

doi:10.3311/ppch.2167

Cited by 15 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The BIOLOG EcoPlate TM method was used to study the substrate utilization patterns of the soil microbial community. The measurement was carried out according to Nagy et al The metabolic fingerprints were analysed with DIALAB EL800 Microplate Reader and the average well colour development (AWCD = ∑( C‐ R )/31, where C is the absorbance value in the control cells and R is the absorbance value in each carbon source well) was calculated.…”

Section: Methodsmentioning

confidence: 99%

Red mud as acidic sandy soil ameliorant: a microcosm incubation study

Ujaczki

Feigl

Farkas

et al. 2016

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Red mud is increasingly considered a valuable by-product requiring development of new re-use technologies instead of deposition as waste. This paper reports the results of a microcosm study aiming to reveal the beneficial effects of the Ajka red mud, as soil ameliorant, on a specific acidic sandy soil in eastern Hungary. RESULTS:The red mud amount mixed into the sandy soil ranged between 0 and 50%. The experiment was monitored by an integrated methodology combining physical and chemical methods with biological and ecotoxicological measurements. The adverse effects of red mud were examined by ecotoxicity tests using test organisms from three trophic levels: Aliivibrio fischeri (luminescent bacterium), Sinapis alba (plant), Triticum aestivum (plant) and Tetrahymena pyriformis (unicellular animal). The results showed that red mud applied at 5% had positive effects on the degraded acidic sandy soil, significantly increasing soil pH, improving soil texture and water holding capacity. In addition, according to the ecotoxicological test results 5% w/w red mud had no significant adverse effects on the tested organisms as members of the soil ecosystem. CONCLUSION:The results demonstrated the beneficial effects of red mud as an ameliorant of acidic sandy soils under continental climatic conditions.

show abstract

Section: Methodsmentioning

confidence: 99%

Red mud as acidic sandy soil ameliorant: a microcosm incubation study

Ujaczki

Feigl

Farkas

et al. 2016

J of Chemical Tech & Biotech

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plates were incubated in the dark at 25 • C, and the absorbance of the wells was measured every 24 h for 120 h at 490 nm wavelength by DIALAB EL800 Microplate Reader. Although the Biolog EcoPlate™ method usually measures optical density (OD) at 590 nm, because the highest absorbance of the tetrazolium dye could be measured at this wavelength [31,34,36], our microplate reader was equipped with 405, 450, 490 and 630 nm filters, and the optimal OD values were provided at 490 nm [37,38].…”

Section: Biolog Ecoplate™mentioning

confidence: 99%

The Biolog EcoPlate™ Technique for Assessing the Effect of Metal Oxide Nanoparticles on Freshwater Microbial Communities

et al. 2021

Self Cite

View full text Add to dashboard Cite

The application of Biolog EcoPlate™ for community-level physiological profiling of soils is well documented; however, the functional diversity of aquatic bacterial communities has been hardly studied. The objective of this study was to investigate the applicability of the Biolog EcoPlate™ technique and evaluate comparatively the applied endpoints, for the characterisation of the effects of metal oxide nanoparticles (MONPs) on freshwater microbial communities. Microcosm experiments were run to assess the effect of nano ZnO and nano TiO2 in freshwater at 0.8–100 mg/L concentration range. The average well colour development, substrate average well colour development, substrate richness, Shannon index and evenness, Simpson index, McIntosh index and Gini coefficient were determined to quantify the metabolic capabilities and functional diversity. Comprehensive analysis of the experimental data demonstrated that short-term exposure to TiO2 and ZnO NPs affected the metabolic activity at different extent and through different mechanisms of action. TiO2 NPs displayed lower impact on the metabolic profile showing up to 30% inhibition. However, the inhibitory effect of ZnO NPs reached 99% with clearly concentration-dependent responses. This study demonstrated that the McIntosh and Gini coefficients were well applicable and sensitive diversity indices. The parallel use of general metabolic capabilities and functional diversity indices may improve the output information of the ecological studies on microbial communities.

show abstract

“…AWCD indicates the total metabolic capacity of sediment microbial communities in terms of carbon-source utilization. The OD values for data evaluation and comparisons were used at 120hr since these represented the optimal range of OD readings in our study (Gryta et al 2014;Nagy et al 2013).…”

Section: Community-level Physiological Pro Ling (Clpp)mentioning

confidence: 99%

Spatio-temporal variations in the physiological profiles of streambed bacterial communities: implication of wastewater treatment plant effluents

Akinwole

Guta

Draper

et al. 2021

World J Microbiol Biotechnol

View full text Add to dashboard Cite

The e uents of wastewater treatment plants (WWTPs) represent a complex mixture of nutrients and toxic substances, thus, the potential exists for the e uents to signi cantly impact the biochemical characteristics and bacterial communities of the receiving water. We examined spatial and seasonal patterns, and the impact of e uents on microbial biomass, bacterial community structure, and metabolic diversity on a fourth-order stream. We took triplicate sediment samples at ve different locations along a 5,000m transect over three sampling periods. We quanti ed bacterial community structure as communitylevel physiological pro les and biomass with phospholipid phosphate analysis. Our ndings highlight the worrisome impacts of e uents on microbial biomass and bacterial metabolic diversity on the receiving water. Microbial biomass was signi cantly higher at the WWTP outfall compared to upstream and downstream sites and correlated positively with sediment physicochemical parameters. Furthermore, our data revealed signi cant spatial differences in bacterial community structure in the context of WWTP impact. High nutrient availability (lower carbon/nitrogen ratios) at the outfall increased site-speci c bacterial metabolic diversity in winter but decreased the same in fall. Seasonal changes in the sedimentary microbial biomass and bacterial carbon substrate utilization were evident regardless of the spatial variations or impacts of the wastewater e uents. Communities in fall showed more versatile substrate utilization patterns than the winter communities. These results suggest that WWTP e uents signi cantly increased microbial biomass and highlight its mixed effects on bacterial community structure and metabolic diversity. Also, our data underscore a close association between sedimentary physicochemical parameters and the associated microbial functional activities.

show abstract

Comparative evaluation of microbial and chemical methods for assessing 4-chlorophenol biodegradation in soil

Cited by 15 publications

References 31 publications

Red mud as acidic sandy soil ameliorant: a microcosm incubation study

Red mud as acidic sandy soil ameliorant: a microcosm incubation study

The Biolog EcoPlate™ Technique for Assessing the Effect of Metal Oxide Nanoparticles on Freshwater Microbial Communities

Spatio-temporal variations in the physiological profiles of streambed bacterial communities: implication of wastewater treatment plant effluents

Contact Info

Product

Resources

About