Comparative evaluation of impedanciometry combined with chromogenic agars or RNA hybridization and real-time PCR methods for the detection of L. monocytogenes in dry-cured ham

Abstract

Comparative evaluation of impedanciometry combined with chromogenic agars or RNA hybridization and real-time PCR methods for the detection of L. monocytogenes in dry-cured ham, Food Control (2018),

In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-Producing Escherichia coli, Listeria monocytogenes and Salmonella spp. in Raw Meats

“…The authors of [ 15 ] evaluated the effectiveness of ISO 6579 and VIDAS UP Salmonella SPT in slaughtered sheep, reporting values of 94.44% for SE alt and 99.79% for RT. Relatively high values of SE alt and RT were reported by [ 17 ] who evaluated RT-PCR to detect L. monocytogenes contaminated in dry-cured ham. [ 16 ] investigated a loop-mediated amplification combined with a standard culturing procedure to detect Salmonella enterica in soya meal recording results of 100% for both SE alt and RT.…”

“…Several publications have recently reported the evaluation of RLOD values for alternative methods according to ISO 16140-2: 2016. For example, [ 17 ] evaluated RT-PCR to specifically detect L. monocytogenes present in sliced dry-cured ham at three levels of contamination (0, 0.3 and 0.9 CFU/25 g). They obtained an RLOD value of 1.00 which was below the acceptability limit.…”

“…Such international standards are used to prove that the performance characteristics of a newly developed method are at least equivalent or better than those of a standard method. In addition, few publications have reported on ISO 16140-2: 2016-based validations of different alternative methods to detect only single target bacterium in various food samples [ 15 , 16 , 17 ].…”

Preparation of Fluorescent Molecularly Imprinted Polymers via Pickering Emulsion Interfaces and the Application for Visual Sensing Analysis of Listeria Monocytogenes

“…Aside from its high fatality rate (20–30%), listeriosis can cause many diseases such as sepsis and meningitis. It readily infects immunocompromised people and in particular neonates, pregnant women, and people over age 65 [6,7]. Meats, fruits, vegetables, seafood, milk, and dairy products are common foods associated with Listeria monocytogenes [8,9].…”