Preparation of Fluorescent Molecularly Imprinted Polymers via Pickering Emulsion Interfaces and the Application for Visual Sensing Analysis of Listeria Monocytogenes

Zhao, Xiaolei; Cui, Yan; Wang, Junping; Wang, Junying

doi:10.3390/polym11060984

Cited by 42 publications

(29 citation statements)

References 40 publications

(43 reference statements)

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“…According to WHO (2015), almost 600 million disease cases caused by the consumption of contaminated food by pathogenic bacteria. Salmonella sp., Escherichia coli O157:H7, Staphylococcus aureus, Listeria monocytogenes, and Bacillus cereus are known pathogens in food [1,2]. Those bacteria are a threat to ready-to-eat foods.…”

Section: Introductionmentioning

confidence: 99%

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Astantri

Prakoso

Triyana

et al. 2020

Veterinary Sciences

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The aim of this study is to determine the performance of a lab-made electronic nose (e-nose) composed of an array of metal oxide semiconductor (MOS) gas sensors in the detection and differentiation of Listeria monocytogenes (L. monocytogenes) and Bacillus cereus (B. cereus) incubated in trypticsoy broth (TSB) media. Conventionally, the detection of L. monocytogenes and B. cereus is often performed by enzyme link immunosorbent assay (ELISA) and polymerase chain reaction (PCR). These techniques require trained operators and expert, expensive reagents and specific containment. In this study, three types of samples, namely, TSB media, L. monocytogenes (serotype 4b American Type Culture Collection (ATCC) 13792), and B. cereus (ATCC) 10876, were used for this experiment. Prior to measurement using the e-nose, each bacterium was inoculated in TSB at 1 × 10 3 -10 4 CFU/mL, followed by incubation for 48 h. To evaluate the performance of the e-nose, the measured data were then analyzed with chemometric models, namely linear and quadratic discriminant analysis (LDA and QDA), and support vector machine (SVM). As a result, the e-nose coupled with SVM showeda high accuracy of 98% in discriminating between TSB media and L. monocytogenes, and between TSB media and B. cereus. It could be concluded that the lab-made e-nose is able to detect rapidly the presence of bacteria L. monocytogenes and B. cereus on TSB media. For the future, it could be used to identify the presence of L. monocytogenes or B. cereus contamination in the routine and fast assessment of food products in animal quarantine.Listeria monocytogenes causes the highest case of hospitalization (up to 91%) among other foodborne illnesses [4]. Listeriosis is infectious to humans and mammals, including the ruminant and monogastric animals. The clinical signs of listeriosis in humans include gastroenteritis, diarrhea, meningitis, bacteremia, and it causes encephalitis, septicemia, abortion, mastitis, and gastroenteritis in cows [5,6]. Member of genus Listeria is a non-spore bacterium, being anaerobic facultative, a small size, Gram-positive, and rod-shaped (0.5-4.0 µm diameter and 0.5-2.0 µm long). Listeria monocytogenes can contaminate a wide range of foods, including yogurt, cheese, meat, ham, smoked salmon, poultry, seafood and vegetable products [2,7].Bacillus cereus is a facultative aerobic to anaerobic, Gram-positive, rod-shaped, and spore-forming bacteria. Spore endurance to unfavorable conditions has assisted the widespread of Bacillus [8,9]. Although the culture method is the gold standard for bacteria identification, it is inefficient, time-consuming (more than 1 week), requires laboratory operator expertise, and identification depends on specific microbiological and biochemical testing [7,9,10]. Besides these methods, there are also other detection methods such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). However, the PCR-based technique requires sophisticated equipment, complicated techniques, and lengthy processes such as ...

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Section: Introductionmentioning

confidence: 99%

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Astantri

Prakoso

Triyana

et al. 2020

Veterinary Sciences

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“…We observed that the fluorescence intensity of PABA gradually decreased with increasing BPO concentration (Figure 5A) and the negative logarithm of BPO concentration was proportional to the quenching in PABA fluorescence intensity (F 0 –F i ) with a linear relationship of Y = −198.94 X + 1819.44 (R 2 = 0.9941) in the detection range of 8.26 × 10 −9 M – 8.26 × 10 −4 M (Figure 5B). The LOD is usually obtained from three times of signals to noise (S/N = 3) [8,35,36,37,38] and the estimated LOD was 1.06 × 10 −9 M, featuring a high sensitivity. With the developed method, the ultrasensitive detection for BPO can be completed within a short time.…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive Detection of Benzoyl Peroxide Based on Organoboron Fluorescent Conjugated Polymers

Yin

Zhang

et al. 2019

Polymers

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The method capable of rapid and sensitive detection of benzoyl peroxide (BPO) is necessary and receiving increasing attention. In consideration of the vast signal amplification of fluorescent conjugated polymers (FCPs) for high sensitivity detection and the potential applications of boron-containing materials in the emerging sensing fields, the organoboron FCPs, poly (3-aminophenyl boronic acid) (PABA) is directly synthesized via free-radical polymerization reaction by using the commercially available 3-aminophenyl boronic acid (ABA) as the functional monomer and ammonium persulfate as the initiator. PABA is employed as a fluorescence sensor for sensing of trace BPO based on the formation of charge-transfer complexes between PABA and BPO. The fluorescence emission intensity of PABA demonstrates a negative correlation with the concentration of BPO. And a linear range of 8.26 × 10−9 M–8.26 × 10–4 M and a limit of detection of 1.06 × 10–9 M as well as a good recovery (86.25%–111.38%) of BPO in spiked real samples (wheat flour and antimicrobial agent) are obtained. The proposed sensor provides a promising prospective candidate for the rapid detection and surveillance of BPO.

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“…Finally, the obtained polymers are eluted under appropriate conditions to elute the template molecules. Thus, molecularly imprinted polymers with imprinted holes matched with the size, shape, and functional groups of the template molecules are obtained [1,2]. Pauling, a famous scholar, put forward the theory of antibody formation in the 1940s, which laid a theoretical foundation for the generation of molecular imprinting technology [3].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Molecularly Imprinted Polymer via Emulsion Polymerization for Application in Solanesol Separation

Zhao

Liu

et al. 2020

Applied Sciences

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High-purity solanesol can be used for pharmaceutical applications, but the current method for purifying solanesol has high cost and difficult continuous operation, and the use of molecular imprinting to purify natural products is a hot research topic of current research. Solanesol molecularly imprinted polymers were synthesized via emulsion polymerization for the first time. The morphology of the SSO-MIPs was observed with a scanning electron microscope, and the effects of the synthesis time, initiator dosage, functional monomer dosage, and cross-linking agent dosage on the adsorption effects under high-temperature and rapid synthesis conditions were discussed. The results demonstrate that the optimum synthesis conditions were a ratio of the template molecules to the functional monomers to the cross-linking agents of 1:8:30 (mol:mol:mol), 10 mg of the initiator, and a synthesis temperature of 70 °C. The imprinting factor of SSO-MIPs synthesized under the optimized process was found to reach 2.51, and the SSO-MIPs synthesized by this method exhibited a good adsorption effect, emitted less pollution during the synthesis process, and are convenient for demulsification. This research reports a reliable method for the synthesis of solanesol molecularly imprinted polymers.

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Preparation of Fluorescent Molecularly Imprinted Polymers via Pickering Emulsion Interfaces and the Application for Visual Sensing Analysis of Listeria Monocytogenes

Cited by 42 publications

References 40 publications

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Highly Sensitive Detection of Benzoyl Peroxide Based on Organoboron Fluorescent Conjugated Polymers

Synthesis of Molecularly Imprinted Polymer via Emulsion Polymerization for Application in Solanesol Separation

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