Comparative evaluation of ELISA kits’ reliability for the aflatoxin M1 determination in goat milk

Christoforidou, Sofia; Malissiova, Eleni; Gortzi, Olga; Hadjichristodoulou, Christos

doi:10.1007/s00217-014-2374-x

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…Currently, a number of HPLC-MS or MS/MS equipment are used world-wide to gain a detailed overview on the mycotoxin spectra in feeds and foods depending on laboratory capabilities (Berthiller et al, 2018). At the same time, ELISA methods and equipment are used for quick mycotoxin measurements (Christoforidou et al, 2015;Xu et al, 2015;Sineque et al, 2017). New developments in this field have been published in the latest literature (Pennington, 2017;Udomkun et al, 2017;Yan et al, 2017).…”

Section: Qualitative and Quantitative Aflatoxin Analytical Methods -Ementioning

confidence: 99%

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

et al. 2019

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Section: Qualitative and Quantitative Aflatoxin Analytical Methods -Ementioning

confidence: 99%

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

et al. 2019

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“…Over the last two decades, the application of immunological methods in aflatoxin detection, particularly enzyme-linked immunosorbent assay (ELISA) and immunochramotographic assay (strip), has been favorably accepted [ 12 , 13 ]. ELISA not only is a tool for rapid and sensitive detection with high sample throughput capacity, but also is relatively inexpensive [ 13 , 14 ]. Production of polyclonal antibodies is simple and easy when enough immunogens and animals are available, but monoclonal antibodies (McAbs) have many other advantages, including the capacity for sustainable production and consistent properties.…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins

Zhou

Wang

et al. 2015

Toxins

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To detect and monitor G-group aflatoxins in agricultural products, we generated class-specific monoclonal antibodies that specifically recognized aflatoxins G1 and G2. Of the final three positive and stable hybridomas obtained, clone 2G6 produced a monoclonal antibody that had equal sensitivity to aflatoxins G1 and G2, and did not cross-react with aflatoxins B1, B2, or M1. Its IC50 values for aflatoxins G1 and G2 were 17.18 ng·mL−1 and 19.75 ng·mL−1, respectively. Using this new monoclonal antibody, we developed a competitive indirect enzyme-linked immunosorbent assay (CI-ELISA); the method had a limit of detection of 0.06 ng·mL−1. To validate this CI-ELISA, we spiked uncontaminated peanut samples with various amounts of aflatoxins G1 and G2 and compared recovery rates with those determined by a standard HPLC method. The recovery rates of the CI-ELISA ranging from 94% to 103% were comparable to those of the HPLC (92% to 102%). We also used both methods to determine the amounts of G-group aflatoxins in five peanut samples contaminated by aflatoxin B1-positive, and their relative standard deviations ranged from 8.4% to 17.7% (under 20%), which demonstrates a good correlation between the two methods. We further used this CI-ELISA to assess the ability of 126 fungal strains isolated from peanuts or field soils to produce G-group aflatoxins. Among these, seven stains producing different amounts of G-group aflatoxins were identified. Our results showed that the monoclonal antibody 2 G6-based CI-ELISA was suitable for the detection of G-group aflatoxins present in peanuts and also those produced by fungi.

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“…Currently, a number of HPLC-MS or MS/MS equipment are used world-wide to gain a detailed overview on the mycotoxin spectra in feeds and foods depending on laboratory capabilities (Berthiller et al, 2018). At the same time, ELISA methods and equipment are used for quick mycotoxin measurements (Christoforidou et al, 2015;Sineque et al, 2017). New developments in this field have been published in the latest literature (Pennington, 2017;Yan et al, 2017).…”

Section: Qualitative and Quantitative Aflatoxin Analytical Methods -E...mentioning

confidence: 99%

Aspergillus-Derived Mycotoxins In The Feed And Food Chain

2021

Frontiers Research Topics

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Frontiers is more than just an open-access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers Journal SeriesThe Frontiers Journal Series is a multi-tier and interdisciplinary set of open-access, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers Journal Series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to QualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation.

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Comparative evaluation of ELISA kits’ reliability for the aflatoxin M1 determination in goat milk

Cited by 9 publications

References 27 publications

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

Development of an Enzyme-Linked Immunosorbent Assay Method Specific for the Detection of G-Group Aflatoxins

Aspergillus-Derived Mycotoxins In The Feed And Food Chain

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