Comparative evaluation of dietary probiotics Bacillus subtilis WB60 and Lactobacillus plantarum KCTC3928 on the growth performance, immunological parameters, gut morphology and disease resistance in Japanese eel, Anguilla japonica

Lee, Seunghan; Katya, Kumar; Park, Youngjin; Won, Seonghun; Seong, Minji; Hamidoghli, Ali; Bai, Sungchul C.

doi:10.1016/j.fsi.2016.12.035

Cited by 103 publications

(89 citation statements)

References 53 publications

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“…Probiotics have been used as an alternative environment-friendly strategy to develop sustainable aquaculture and have been defined in several ways; as live and viable microbial food supplements that provide beneficial effects to fish in terms of growth performances, immune responses, intestinal microbial balance and digestive enzyme activities [9,10]. Probiotics such as Bacillus subtilis [11], Baillus licheniformis [12], Lactococcus lactis [13] and Micrococcus luteus [14] have been identified beneficial for aquaculture. Specially, B. subtilis is a common probiotic and it has been reported that 10 7 and 10 8 CFU/g of this bacteria can enhance growth and immune while improving gastrointestinal tract of fish [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

et al. 2020

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An eight-week feeding trial was conducted to evaluate the effects of a basal control diet (CON), Bacillus subtilis at 107 (BS7) and at 108 CFU/g diet (BS8), Lactococcus lactis at 107 CFU/g (LL7) and at 108 CFU/g diet (LL8), and oxytetracycline (OTC) at 4 g/kg diet on Nile tilapia. Fish with initial body weight of 2.83 ± 0.05 g (mean ± SD) were fed two times a day. Weight gain, specific growth rate, feed efficiency, protein efficiency ratio and lysozyme activity of fish fed BS8, LL8 and LL7 diets were significantly higher than those of fish fed CON diet (p < 0.05). Superoxide dismutase and myeloperoxidase activity of fish fed BS8, LL8, BS7, LL7 and OTC diets were significantly higher than those of fish fed CON diet. Intestinal villi length and muscular layer thickness of fish fed BS8, LL8 and LL7 diets were significantly higher than those of fish fed CON and OTC diets. Also, heat shock protein 70 (HSP70), interleukin (IL-1β), interferon-gamma (IFN-γ) and tumour necrosis factor (TNF-α) gene expression of fish fed BS8 and LL8 diets were significantly higher than those of fish fed CON diet. After 13 days of challenge test, cumulative survival rate of fish fed BS8 and LL8 diets were significantly higher than those of fish fed CON, BS7 and OTC diets. Based on these results, B. subtilis and L. lactis at 108 (CFU/g) could replace antibiotics, and have beneficial effects on growth, immunity, histology, gene expression, and disease resistance in Nile tilapia.

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Section: Introductionmentioning

confidence: 99%

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

et al. 2020

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“…Our previous study assessed isolated B. subtillis WB60 from the intestine of healthy Japanese eel and compared it with Lactobacillus plantarum to examine the dietary effects on several parameters such as growth performance, non‐specific immune responses and disease resistance of this fish. The results showed fish fed B. subtillis at 10 8 CFU/g diet had better growth and immune responses compared with fish fed other levels (10 6 ~ 10 8 CFU/g diet) or L. plantarum assuming that dietary Bacillus subtillis could be the most effective probiotic in Japanese eel (Lee et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of two dietary probiotics ( Bacillus subtilis or licheniformis ) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica

et al. 2019

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A 12‐week feeding trial was conducted to investigate the effects of two dietary probiotics; Bacillus subtilis KCTC 2217 or Bacillus licheniformis KCCM 11775 with two prebiotics; mannan oligosaccharide (MOS) or fructooligosaccharide (FOS) in Japanese eel. Fish averaging 12.8 ± 0.47 g (mean ± SD) were randomly distributed into five treatments with triplicate tanks. A basal control diet (CON) and four synbiotic diets supplementing B. subtilis + MOS (BSM), B. subtilis + FOS (BSF), B. licheniformis + MOS (BLM), and B. licheniformis + FOS (BLF). Weight gain and specific growth rate of fish fed all synbiotic diets were higher than those of fish fed CON. Immune‐related gene expression of heat shock protein 70 and immunoglobulin M of fish fed BSF and BLM were significantly higher than those of fish fed CON. Fish fed BSF and BLM had significantly higher intestinal villi length than those of fish fed BLF and CON (p < .05). Disease resistance against Aeromonas hydrophila of fish fed all synbiotic diets were significantly higher than those of fish fed CON (p < .05). Therefore, these results indicated that dietary B. subtilis with FOS (BSF) and B. licheniformis with MOS (BLM) could have beneficial effects on intestinal morphology, and immune‐related gene expression in Japanese eel.

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“…This outcome is contrary to that of Jesus et al (), who observed higher width, height, number of villi, and numbers of goblet cells in hybrid surubim ( Pseudoplatystoma reticulatum × P. corruscans ) after receiving the probiotic W. cibaria for 45 days. Other studies have reported higher measurements of gut metrics in fish that received probiotic bacteria, for example, Lee et al () in Anguilla japonica after 60 days of experiment and Abid et al () in Salmon salar after 63 days of feeding with probiotic bacteria. This discrepancy could be attributed to the longer feeding period during which the probiotic had been offered to the fish.…”

Section: Discussionmentioning

confidence: 93%

Autochthonous probiotic bacteria modulate intestinal microbiota of Pirarucu, Arapaima gigas

Pereira

Soares³

et al. 2019

J World Aquaculture Soc

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This study aimed to investigate the ability of two probiotic strains (Lactococcus lactis subsp. lactis and Enterococcus faecium) to colonize Arapaima gigas intestines and their competence in modulating gut microbiota, morphology, and immunological status. A total of 135 A. gigas (58.86 ± 10.25 g) were divided into three treatments: (a) fish fed a diet containing L. lactis subsp. lactis at 1 × 108 CFU−1, (b) fish fed a diet containing E. faecium at 1 × 108 CFU−1, and (c) fish fed a diet nonsupplemented with probiotic. After 21 days, 12 fish per treatment were sampled. Blood and posterior intestine were sampled for further analysis. High‐throughput sequencing results demonstrated that the relative abundance of Cetobacterium genus was high in all treatments. The number of thrombocytes increased in both groups fed the probiotic, and eosinophils were higher in fish fed E. faecium. Serum antimicrobial activity of fish fed L. lactis subsp. lactis was higher when compared with the other two treatments. In conclusion, both strains could populate the intestinal mucosa of A. gigas and could be used as probiotics in pirarucu production as they can influence hematoimmunological parameters and decrease the abundance of pathogenic bacteria in the gut of pirarucu within 21 days of feeding.

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Comparative evaluation of dietary probiotics Bacillus subtilis WB60 and Lactobacillus plantarum KCTC3928 on the growth performance, immunological parameters, gut morphology and disease resistance in Japanese eel, Anguilla japonica

Cited by 103 publications

References 53 publications

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

Effects of Bacillus subtilis WB60 and Lactococcus lactis on Growth, Immune Responses, Histology and Gene Expression in Nile Tilapia, Oreochromis niloticus

Effects of two dietary probiotics ( Bacillus subtilis or licheniformis ) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica

Autochthonous probiotic bacteria modulate intestinal microbiota of Pirarucu, Arapaima gigas

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