Comparative Evaluation of Conventional Blood Culture with Bactec 9050 for Bacterial Isolates in Clinically Suspected Cases of Fever of Unknown Origin

Kaur, Avneet; Soodan, Puneet Singh; Singh, Varsha

doi:10.9790/0853-13711721

Cited by 7 publications

(16 citation statements)

References 9 publications

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“…[14] In present study, males constituted majority (53.7%) of the patients with male to female ratio of 1.16:1 from rural background (56%). This finding was similar with Avneet Kaur et al 2014 [7] who reported male predominance (65.22%) with rural background (65.22%). Gopi et al…”

Section: Discussionsupporting

confidence: 91%

“…Though newer techniques like nucleic acid probes, polymerase chain reaction and other molecular techniques are available; blood culture still remains the most practical and reliable method in the diagnosis of bloodstream infections. [7,8] Blood cultures provide the best yield for microbiological diagnosis, with sensitivity ranging from 53% to 90%. [9] Conventional blood culture methods use culture media like brain heart infusion broth, tryptic soy broth, bile broth, glucose broth etc.…”

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confidence: 99%

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Comparative Study on Conventional Blood Culture and Automated Blood Culture (Bactec 9050) in the Early Detection of Bacterial Isolates in Tertiary Care Hospital of Kumaun Region

Anjum¹,

Umesh²,

Rawat³

et al. 2019

jemds

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BACKGROUND Blood stream infections range from self-limiting infections to life threatening sepsis that require rapid and aggressive antimicrobial treatment. Timely detection and identification of blood-borne pathogens would be a useful guide for clinicians in initiating the empiric antibiotic therapy. Objectives-To evaluate the capability, efficiency and reliability of automated blood culture methods (BACTEC 9050) in comparison to conventional blood culture for detection of bacterial isolates in clinically suspected cases of septicaemia. METHODSAll the blood culture samples (in duplicate), from 2 different sites, at 2 different times, 30 minutes apart, were taken from suspected cases of septicaemia consecutively during study period (September 2016 to June 2017). Samples were subjected to conventional blood culture and BACTEC 9050 culture system. RESULTSOut of 254 suspected cases of septicaemia, 93 (36.6%) cases were culture positive. Among these, 60% were positive with both methods while 36.5% were positive on BACTEC culture only. Out of 93 positive cases, a total of 100 isolates comprising of grampositive bacteria (62%), gram-negative bacteria (36%) and Candida sp. (2%) were detected. BACTEC 9050 detected all positive samples in within 24 hours while Conventional method detected none within 24 hrs, 25.4% within 48 hours, and 84.7% within 86 hours. Among gram-positive bacteria, predominant isolates were Coagulase Negative Staphylococcus (41%) followed by Enterococcus (9%). Among gram-negative isolates, 14% were Pseudomonas sp. followed by 10% Acinetobacter sp. BACTEC 9050 was observed to be more sensitive (94.9%) in comparison to conventional blood culture. Mean time of detection was significantly less (11.3 hours) with the BACTEC 9050 than with conventional method (61.7 hours). CONCLUSIONSBACTEC 9050 proved to be a reliable, fast technique with high sensitivity and specificity in identification of the blood stream pathogens in blood culture in comparison to conventional culture methods. HOW TO CITE THIS ARTICLE: Anjum N, Umesh, Rawat V, et al. Comparative study on conventional blood culture and automated blood culture (BACTEC 9050) in the early detection of bacterial isolates in tertiary care hospital of Kumaun region.

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Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

Comparative Study on Conventional Blood Culture and Automated Blood Culture (Bactec 9050) in the Early Detection of Bacterial Isolates in Tertiary Care Hospital of Kumaun Region

Anjum¹,

Umesh²,

Rawat³

et al. 2019

jemds

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“…[1921] A few studies, however, have reported a higher Gram-positive isolation. [620] This may be attributed to possible geographical variations in antimicrobial spectrum. Individual pathogen comparison between the two systems showed significantly higher recovery of Acinetobacter spp, Streptococcus spp, and CoNS [Table 3] by BACTEC 9050.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies have reported a mean detection time of 19–24 h hours by BACTEC and 5–7 days by conventional method. [6182022] Since BACTEC is a continuous monitoring system, detection occurs earlier and can be reported in hours. However, conventional blood culture, by its very nature of processing, cannot be reported positive before 24 h and that too when a Gram's stained primary smear reveals the presence of microorganisms.…”

Section: Discussionmentioning

confidence: 99%

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An appropriately performed conventional blood culture can facilitate choice of therapy in resource-constrained settings-comparison with BACTEC 9050

Surase

Nataraj

Pattamadai

et al. 2016

Journal of Postgraduate Medicine

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Aims:Comparison of conventional blood culture with BACTEC 9050 for rate and time to detection of microorganisms.Settings and Design:A prospective study was carried out in a multispecialty tertiary care teaching hospital.Subjects and Methods:A total of 835 paired specimens (797 blood and 38 nonblood specimens) were collected and processed according to standard microbiological procedures by both conventional method as well as by BACTEC 9050 automated culture system. Clinical details of patients were recorded. Data were analyzed for time to detection and isolation rate by the two systems and compared.Results:Overall culture positivity for BACTEC 9050 and the conventional system was 32% and 19.88%, respectively. Eighty-five demonstrated concordant growth, 136 specimens were culture positive by BACTEC only, and 38 specimens were culture positive by conventional only. Twelve contaminants in BACTEC and nine contaminants in conventional system were detected. Using BACTEC 9050, higher isolation was observed for Acinetobacter spp., coagulase negative Staphylococcus spp., Streptococcus spp., and Candida spp. A total of 410 patients were on antimicrobial treatment and culture positivity was significantly higher with BACTEC 9050 (P < 0.0001). There was a significant difference in the mean time to detection with BACTEC 9050 recovering 86.8% of isolates within 48 h (P < 0.0001).Conclusions:Although BACTEC 9050 demonstrated a significantly higher recovery of microorganisms from blood, an appropriately performed conventional blood culture can facilitate the choice of therapy.

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2023

IJPSR

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Aim & Background: Body fluids are sent to clinical microbiology laboratory for culture to find the etiological agent causing the infection. This study was conducted to compare the culture results of sterile body fluids processed simultaneously by both conventional method and BACTEC blood culture system. Methods: Sterile body fluids except blood were included in the study. A total of 61 body fluid samples were received and simultaneously processed by both the culture methods-conventional and BACTEC. Results: In this study, overall culture positivity for BACTEC was 85.25% as compared to 59.02% by conventional system. According to the results of our study, the overall culture positivity was increased by 26.23% by BACTEC method than the conventional method. BACTEC blood culture system detected more pathogenic isolates (52.45%) than the routine conventional culture method (21.31%) and it was found to be statistically significant (p value=<0.005). The most common pathogenic micro-organisms detected by conventional and BACTEC methods in this study were Gram-negative followed by Gram-positive. The mean time to detection of pathogenic isolates by BACTEC and conventional method was 1.19(+/-0.39) days and 2.00(+/-1.00) days and the difference was statistically significant (p value=0.013). Conclusion: The study recommends the use of automated blood culture system for culture of sterile body fluids as it was found to improve the yield of isolates with reduced time to detection. However, cost may be a limiting factor in resource constrained settings. INTRODUCTION:Sterile body fluid infections can be caused by both Gram-positive and Gramnegative bacteria. Gram-negative bacteria like Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumanii and Pseudomonas aeruginosa are the frequently isolated pathogens followed by Gram-positive bacteria like Staphylococcus aureus and Enterococcus 1-4 . Presence of microorganisms in normally sterile body sites causes life-threatening infections 2, 5 .

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Comparative Evaluation of Conventional Blood Culture with Bactec 9050 for Bacterial Isolates in Clinically Suspected Cases of Fever of Unknown Origin

Abstract: Abstract

Cited by 7 publications

References 9 publications

Comparative Study on Conventional Blood Culture and Automated Blood Culture (Bactec 9050) in the Early Detection of Bacterial Isolates in Tertiary Care Hospital of Kumaun Region

Comparative Study on Conventional Blood Culture and Automated Blood Culture (Bactec 9050) in the Early Detection of Bacterial Isolates in Tertiary Care Hospital of Kumaun Region

An appropriately performed conventional blood culture can facilitate choice of therapy in resource-constrained settings-comparison with BACTEC 9050

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