Comparative Evaluation of Broth Microdilution with Polystyrene and Glass-Coated Plates, Agar Dilution, E-Test, Vitek, and Disk Diffusion for Susceptibility Testing of Colistin and Polymyxin B on Carbapenem-Resistant Clinical Isolates of <i>Acinetobacter baumannii</i>

Singhal, Lipika; Sharma, Megha; Verma, Shreya; Kaur, Ramanpreet; Xavier, Basil Britto; Kumar, Surbhi Malhotra; Ray, Pallab; Gautam, Vikas

doi:10.1089/mdr.2017.0251

Cited by 40 publications

(30 citation statements)

References 23 publications

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“…These type of microtubes showed the least adsorption, followed by standard polypro-pylene and glass. Similar results have also been obtained from other studies, demonstrating that BMD with glass-coated plates gives more reliable MIC results for polymyxins than BMD with polystyrene plates (96,123). Furthermore, Karvanen et al (103) previously showed that colistin adsorption was proportionally higher at a lower concentration of 0.125 g/ml after 24 h at 35°C, with the estimated colistin concentration in MHB being 8%, 13%, and 25% in polystyrene, polypropylene, and glass-coated wells, respectively.…”

Section: Challenges In Polymyxin Susceptibility Testingsupporting

confidence: 86%

Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

2019

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Polymyxins, including polymyxin B and polymyxin E (colistin), are now increasingly being used worldwide to treat patients with multidrug-resistant (MDR) Gram-negative bacterial infections. This necessitates that laboratories employ an accurate and reliable method for the routine performance of polymyxin susceptibility testing. A number of reasons have accounted for the difficulties with susceptibility testing for the polymyxins, including their multicomponent composition, poor diffusion in the agar medium, adsorption to microtiter plates, the lack of a reliable susceptibility test, the lack of a specific breakpoint from professional organizations, the synergistic effect of polysorbate 80, and the development of heteroresistance. This minireview discusses such problems that impact the results of currently available susceptibility testing methods. We also provide emerging concepts on mechanisms of polymyxin resistance, including chromosomally and plasmid-mediated mcr-related resistance. Broad-range investigations on such critical issues in relation to polymyxins can be beneficial for the implementation of effective treatment against MDR Gram-negative bacterial infections.

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Section: Challenges In Polymyxin Susceptibility Testingsupporting

confidence: 86%

Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

2019

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“…Albur et al demonstrated that the polystyrene trays used also have an influence: using tissue-culture-coated round-bottom trays gave a 5.3-fold increase in MIC values compared to noncoated V-bottom trays [113], for the material used [106] (Table S3). A very recent study compared polystyrene coated trays to glass coated trays and also showed very few differences (Table 3) [114].…”

Section: New Recommendations On Polymyxins Susceptibility Testingmentioning

confidence: 99%

Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains

Bardet

Rolain

2018

Canadian Journal of Infectious Diseases and Medical Microbiolog

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The recent discovery of the plasmid-mediated mcr-1 gene conferring resistance to colistin is of clinical concern. The worldwide screening of this resistance mechanism among samples of different origins has highlighted the urgent need to improve the detection of colistin-resistant isolates in clinical microbiology laboratories. Currently, phenotypic methods used to detect colistin resistance are not necessarily suitable as the main characteristic of the mcr genes is the low level of resistance that they confer, close to the clinical breakpoint recommended jointly by the CLSI and EUCAST expert systems (S ≤ 2 mg/L and R > 2 mg/L). In this context, susceptibility testing recommendations for polymyxins have evolved and are becoming difficult to implement in routine laboratory work. The large number of mechanisms and genes involved in colistin resistance limits the access to rapid detection by molecular biology. It is therefore necessary to implement well-defined protocols using specific tools to detect all colistin-resistant bacteria. This review aims to summarize the current clinical microbiology diagnosis techniques and their ability to detect all colistin resistance mechanisms and describe new tools specifically developed to assess plasmid-mediated colistin resistance. Phenotyping, susceptibility testing, and genotyping methods are presented, including an update on recent studies related to the development of specific techniques.

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“…A number of publications have demonstrated that the results of AST of many antimicrobials, not just AMP, can be affected by the choice of laboratory plasticware for use with the broth microdilution procedure and even the choice of tubes used for preparing reagents (Singhal et al, 2018 ; Kavanagh et al, 2019 ). This also applies to AST of AMP (Otvos and Cudic, 2007 ; Wiegand et al, 2008 ; Kristensen et al, 2015 ).…”

Section: Antimicrobial Susceptibility Testing Of Ampmentioning

confidence: 99%

Antimicrobial Susceptibility Testing of Antimicrobial Peptides to Better Predict Efficacy

Mercer

Torres

Duay

et al. 2020

Front. Cell. Infect. Microbiol.

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During the development of antimicrobial peptides (AMP) as potential therapeutics, antimicrobial susceptibility testing (AST) stands as an essential part of the process in identification and optimisation of candidate AMP. Standard methods for AST, developed almost 60 years ago for testing conventional antibiotics, are not necessarily fit for purpose when it comes to determining the susceptibility of microorganisms to AMP. Without careful consideration of the parameters comprising AST there is a risk of failing to identify novel antimicrobials at a time when antimicrobial resistance (AMR) is leading the planet toward a post-antibiotic era. More physiologically/clinically relevant AST will allow better determination of the preclinical activity of drug candidates and allow the identification of lead compounds. An important consideration is the efficacy of AMP in biological matrices replicating sites of infection, e.g., blood/plasma/serum, lung bronchiolar lavage fluid/sputum, urine, biofilms, etc., as this will likely be more predictive of clinical efficacy. Additionally, specific AST for different target microorganisms may help to better predict efficacy of AMP in specific infections. In this manuscript, we describe what we believe are the key considerations for AST of AMP and hope that this information can better guide the preclinical development of AMP toward becoming a new generation of urgently needed antimicrobials.

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Comparative Evaluation of Broth Microdilution with Polystyrene and Glass-Coated Plates, Agar Dilution, E-Test, Vitek, and Disk Diffusion for Susceptibility Testing of Colistin and Polymyxin B on Carbapenem-Resistant Clinical Isolates of Acinetobacter baumannii

Abstract: Different susceptibility testing methods for polymyxins show great variation in their results and BMD using glass-coated plates can be considered the best candidate for gold standard.

Cited by 40 publications

References 23 publications

Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

Antimicrobial Susceptibility Testing for Polymyxins: Challenges, Issues, and Recommendations

Development of New Tools to Detect Colistin-Resistance among Enterobacteriaceae Strains

Antimicrobial Susceptibility Testing of Antimicrobial Peptides to Better Predict Efficacy

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