Comparative Efficiency Analysis of Different Explants and Contribution of Polyamines on in vitro Propagation of Dendrobium Hybrid Sonia

Mandal, Sanjay K.; Pal, Nishith Kumar; Mondal, Tustu; Banerjee, Nirmalya

doi:10.3329/ptcb.v30i1.47793

Cited by 3 publications

(4 citation statements)

References 14 publications

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“…It was observed that when MS medium supplemented with the combination of BAP and NAA, stimulated the formation of EC after 6 weeks of culture and further development of SEs were observed in lower concentrations. This finding could be corelated with the effect of BAP and NAA to induce maximum number of PLBs from thin cell layer culture of Dendrobium hybrid Sonia (Mandal et al 2020). The similar synergistic effect of BAP and NAA were also reported in Dendrobium palpebrae (Bhowmik and Rahman, 2020) and Cymbidium finlaysonianum (Shahinul Islam et al 2015).…”

Section: Pyatisupporting

confidence: 77%

“…This is advantageous to pharmaceutical industry that uses orchids as raw material in bulk quantity. Different plant tissues such as shoot tips (Mamun et al 2018;Mandal et al 2020) protocorm like bodies (Ashok Pyati 2020, Klaocheed et al 2021) pseudobulbs (Bowmik and Rahman 2020), nodal explants (Bhattacharyya et al 2016, Kaur 2017, leaf and roots (Lee and Chen 2014) stem thin cell layers (Parthibhan et al 2018) have been widely used as explants to obtain regenerative plantlets in several Dendrobiums. In D. ovatum different tissue culture systems for micropropagation have been established using mature and immature seeds as explants (Gurudeva 2019, Deeksha Raj et al 2021, Shetty et al 2015, Thejaswini and Narasimhan, 2017, Pujari et al 2021, Mary and Divakar 2015.…”

Section: Introductionmentioning

confidence: 99%

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In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Pyati¹

2022

Plant Tissue Cult. & Biotech.

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An efficient in vitro regeneration protocol through somatic embryogenesis was established from longitudinally bisected protocorm (lTCL) of an endangered orchid Dendrobium ovatum. The efficiency of EC and SEs from the protocorms significantly relied on the concentration of PGRs. MS medium supplemented with TDZ (1.0 mg/l) induced optimum of EC (31.8%) and SEs (28.1/explant). Similarly, ZEA (0.5 mg/l) induced EC (27.6%) and SEs (17.1/explant). The combined treatment of TDZ (1.0 mg/l) and NAA (0.5 mg/l) resulted in the maximum induction of EC (58.6%) and SEs (39.8/explant) in an upright incubated explant. In another combined effect of ZEA (1.0 mg/l) and NAA (0.5 mg/l) induced EC (43.8%) and SEs (24.3/explant), whereas the combination of BAP (0.5 mg/l) and NAA (0.5 mg/l) produced EC (34.4%) and SEs (16.8/explants). The induced EC and SEs where healthier on medium containing TDZ + NAA than on the medium containing ZEA+NAA and BAP+NAA. The orientation of lTCL explants seemed to interact with position to affect the morphogenesis. The lTCL explants incubated in the upright orientation induced higher percentage of EC and a greater number of SEs/explants (EC-58.6% and SEs-39.8/explants) than that in the inverted orientation (EC- 16.4% and SEs-9.1/explants) irrespective of the PGRs. The SEs developed from the EC and the intermediate stage of PLBs were finally differentiated into plantlets. About 89% of plantlets were successfully acclimatized in the green house conditions. Plant Tissue Cult. & Biotech. 32(1): 53-66, 2022 (June)

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Section: Pyatisupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Pyati¹

2022

Plant Tissue Cult. & Biotech.

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“…The addition of PAs (1.0 µM of Put and Spd) to liquid MS medium induced direct PLB formation with a highest frequency of 80%, and associated with 8 µM BA enhanced the direct PLB formation in shoot tip culture of Dendrobium hybrid Sonia. While the control with only 8 µM BA showed no direct PLB formation of Dendrobium(Mandal et al 2020), in the present study, using the same concentration of BA, 89% of PLBs regeneration with apical tTCLs was obtained. We only evaluated the responses of PGRs and isolated PAs, requiring experiments of the combination to verify the effect on the PLBs regeneration of C. warneri.…”

contrasting

confidence: 48%

Effect of microalga Desmodesmus subspicatus, polyamines and plant growth regulators on the in vitro propagation of Cattleya warneri

Navarro

Corrêa

Behling

et al. 2022

Preprint

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Cattleya warneri, an orchid with high ornamental potential, suffers indiscriminate harvest and is classified as a vulnerable species, requiring propagation studies. The aim of this study was to evaluate the effect of biomass or aqueous extract of Desmodesmus subspicatus microalga, polyamines (PAs) and plant growth regulators (PGRs): 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA) on the in vitro propagation of C. warneri using the thin cell layer (TCL) technique. Entire protocorms and transversal sections (tTCLs) or longitudinal sections (lTCLs) were grown in MS culture medium, with the concentration of macronutrients reduced by half (MSM/2) containing D. subspicatus biomass or extract Pas, and BA and/or NAA. The results indicated that the apical tTCLs were the best explants and the entire protocorms formed seedlings. The regeneration of protocorm-like bodies (PLBs) was more efficient with the addition of 1.5 g L− 1 of microalgae biomass (92% and 4.7 PLBs) or 1.0 g L− 1 of extract (100% and 3.3 PLBs) and 8 µM BA (89% and 5.2 PLBs). The biomass (1.0 g L− 1) or extract (2.0 g L− 1) also enabled PLBs regeneration from entire protocorms (70% and 4.0 PLBs, 50% and 3.2 PLBs, respectively). The plant acclimatization was recommended using coconut fiber and vermiculite (1:1, v/v) as substrate (survival rate 84%% and greater fresh mass 0.895 g). In conclusion, a rapid and efficient protocol for the mass propagation of C. warneri was achieved using the TCL technique. In addition, MSM/2 medium supplementation with biomass and extract of D. subspicatus is an effective alternative to replace conventional PGRs.

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“…al) added with TDZ (1.0 mg/l) and NAA (0.5 mg/l) induced callus frequency of 77.8% (Ahmed Sherif et al 2016). The thin cell layer culture in Dendrobium Hybrid Sonia induced maximum frequency of callus formation (40%) in MS medium supplemented with NAA (0.5 mg/l) with BAP (0.5 mg/l) (Mandal et al 2020). Until there are many reports about plantlet regeneration from callus cultures via PLB formation in many orchids.…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through Protocorm like bodies.

Pyati¹

2023

Plant Tissue Cult. & Biotech.

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A protocol for regeneration of an endangered medicinal orchid Dendrobium crepidatum was established mainly via indirect protocorm like body (PLBs) formation. The PLBs procured from in vitro growth cultures was assessed in ½ MS medium alone and in combinations with 6-Benzyl amino purine (BAP), Kinetin (Kn) and α-Naphthalene acetic acid (NAA). The calli were induced from longitudinally bisected segments of PLBs. The optimum percentage of callus was obtained in ½ MS medium supplemented with BAP (1.0 mg/l) and combination of BAP (1.0 mg/l) with NAA (0.5 mg/l), where 64.3% and 83.3% of callus was formed respectively. PLB formation was achieved when callus was transferred onto the respective combinations in ½ MS basal medium. The optimum percentage (91.6%) of PLBs were regenerated in the combination of BAP with NAA. These PLBs differentiated into intact plantlets about 3 cm in height and with 4-5 roots were transplanted into potting mixture with highest rate of 93.3% survival rate in a green house.

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Comparative Efficiency Analysis of Different Explants and Contribution of Polyamines on in vitro Propagation of Dendrobium Hybrid Sonia

Cited by 3 publications

References 14 publications

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

In vitro Propagation of orchid (Dendrobium ovatum (L.) Kraenzl.) through Somatic Embryogenesis

Effect of microalga Desmodesmus subspicatus, polyamines and plant growth regulators on the in vitro propagation of Cattleya warneri

In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through Protocorm like bodies.

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