Comparative efficacy of resorbable fiber wraps loaded with gentamicin sulfate or gallium maltolate in the treatment of osteomyelitis

Buie, Taneidra W.; Whitely, Michael; McCune, Joshua; Lan, Ziyang; Jose, Anupriya; Balakrishnan, Annika; Wenke, Joseph C.; Cosgriff‐Hernandez, Elizabeth

doi:10.1002/jbm.a.37210

Cited by 5 publications

(2 citation statements)

References 53 publications

(98 reference statements)

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“…Additionally, fiber microarchitecture can be controlled to vary anisotropy, pore size, and surface texture. 10,[33][34][35][36][37][38] For instance, the fibers can be aligned to support regeneration of anisotropic tissues like skeletal muscle or randomly oriented to support regeneration of isotropic tissue like adipose tissue. The interconnected porosity and high surface area of electrospun scaffolds also provide a tunable platform for cell interaction and infiltration.…”

Section: Introductionmentioning

confidence: 99%

Suspension Electrospinning of Decellularized Extracellular Matrix

Jones,

VandenHeuval,

Martinez

et al. 2024

Preprint

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Decellularized extracellular matrices (dECM) have strong regenerative potential as tissue engineering scaffolds; however, current clinical options for dECM are limited to freeze-drying its native form into sheets. Electrospinning is a versatile scaffold fabrication technique that allows control of macro- and microarchitecture. It remains challenging to electrospin dECM; which has led researchers to either blend it with synthetic materials or use enzymatic digestion to fully solubilize the dECM. Both strategies reduce the innate bioactivity of dECM and limit its regenerative potential. Herein, we developed a new suspension electrospinning method to fabricate a pure dECM scaffold that retains its innate bioactivity. Systematic investigation of suspension parameters was used to identify critical rheological properties required to instill spinnability, including homogenization, concentration, and particle size. Homogenization enhanced particle interaction to impart the requisite elastic behavior to withstand electrostatic drawing without breaking. A direct correlation between concentration and viscosity was observed that altered fiber morphology; whereas, particle size had minimal impact on suspension properties and fiber morphology. The versatility of this new method was demonstrated by electrospinning dECM with three common decellularization techniques (Abraham, Badylak, Luo) and tissue origins (intestinal submucosa, heart, skin). Bioactivity retention after electrospinning was confirmed using cell proliferation, angiogenesis, and macrophage assays. Collectively, these findings provide a framework for researchers to electrospin dECM for diverse tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Suspension Electrospinning of Decellularized Extracellular Matrix

Jones,

VandenHeuval,

Martinez

et al. 2024

Preprint

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“…In this study, by analysing data from a large-scale genome-wide association study (GWAS), we assessed potential causal associations between speci c immune cell pro les and osteomyelitis risk (11). The application of this approach not only enhances our understanding of the etiology of osteomyelitis, but also provides a scienti c basis for the development of new therapeutic strategies (12).…”

Section: Introductionmentioning

confidence: 99%

Osteomyelitis and immune cell phenotypes: a study based on a Mendelian randomisation approach

Long,

Dong,

et al. 2023

Preprint

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Background: Osteomyelitis is a severe bone marrow infection, whose pathogenesis is not fully understood. This study aims to explore the causal relationship between immune cell characteristics and osteomyelitis, in hopes of providing new insights for the prevention and treatment of osteomyelitis. Methods: Based on two independent samples, this study employed a two-sample Mendelian randomization (MR) analysis to assess the causal relationship between 731 immune cell characteristics (divided into 7 groups) and osteomyelitis. Genetic variants were used as proxies for risk factors to ensure the selected instrumental variables meet the three key assumptions of MR analysis. GWAS data for immune characteristics came from the public GWAS catalog, while data for osteomyelitis was sourced from the Finnish database. Results: At a significance level of 0.05, 21 immune phenotypes were identified as having a causal relationship with the development of osteomyelitis. In the B cell group, phenotypes such as Memory B cell %B cell, CD20- %B cell, and Memory B cell %lymphocyte showed a positive causal relationship with osteomyelitis, while Naive-mature B cell %B cell and IgD- CD38- AC phenotypes showed a negative causal relationship. In addition, specific immune phenotypes in the cDC cell group, Myeloid cell group, TBNK cell group, T cell maturation stage, and Treg cell group also showed significant associations with osteomyelitis. Through reverse MR analysis, it was found that osteomyelitis had no significant causal impact on these immune phenotypes, suggesting that the occurrence of osteomyelitis might not in turn affect these immune cell phenotypes. Conclusion: This study reveals for the first time the causal relationship between specific immune cell characteristics and the development of osteomyelitis, providing a new perspective for understanding the immune mechanism of osteomyelitis. These findings are significant for formulating targeted prevention and treatment strategies, and hold promise for improving the clinical treatment outcomes of patients’ osteomyelitis.

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Silk fibroin/polycaprolactone nanofibrous membranes loaded with natural Manuka honey for potential wound healing

Lan

Zhang

et al. 2021

J of Applied Polymer Sci

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Antibacterial wound dressing, an effective barrier between the wound and the foreign environment, has the ability to inhibit and even kill the bacteria around the wound so that it can accelerate the wound healing. However, the loaded antibacterial agents of antibiotics and metal ions on wound dressings caused the new problems of drug resistance and toxicity. To develop a novel wound dressing with the natural antibacterial property agent, here, natural Manuka honey (MH), silk fibroin (SF), and polycaprolactone (PCL) were mixed to prepare nanofibrous membranes by electrospinning. The SEM observation showed the good microscopic morphology of SF/PCL/MH nanofibrous membranes that the fibers were even and smooth. With the addition of MH, the membranes gained the accepted stress and the excellent flexibility to adapt to wound contour. In addition, their hydrophilic property was beneficial to cell adhesion and proliferation. Most importantly, SF/PCL/MH nanofibrous membranes exhibited excellent sustained release effect and antibacterial properties.The antibacterial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans reached 99.88%, 99.70%, and 98.95%, respectively when 75% natural antimicrobial agent MH was loaded. Moreover, SF/PCL/MH membranes showed good cytocompatibility. These results indicated the fabricated SF/PCL/MH nanofibrous membranes are a kind of novel dressing with good antimicrobial property.

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Comparative efficacy of resorbable fiber wraps loaded with gentamicin sulfate or gallium maltolate in the treatment of osteomyelitis

Cited by 5 publications

References 53 publications

Suspension Electrospinning of Decellularized Extracellular Matrix

Suspension Electrospinning of Decellularized Extracellular Matrix

Osteomyelitis and immune cell phenotypes: a study based on a Mendelian randomisation approach

Silk fibroin/polycaprolactone nanofibrous membranes loaded with natural Manuka honey for potential wound healing

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