Comparative efficacy of macrolides containing marine actinomycetes formulation versus ciprofloxacin ophthalmic solution in controlling <i>Pseudomonas aeruginosa</i> induced conjunctivitis on rabbit model

Wahaab, Femina; Subramaniam, Kalidass; Jawahar, Morris

doi:10.1016/j.ijvsm.2015.08.003

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…In the present study, resistance of P. aeruginosa strains to Kanamycin, and Tetracycline were 91.8%, and 94.5%, respectively, similar to results of Wahaab et al 21 study, who recorded resistance to those antibiotics were 100%. Resistance of P. aeruginosa strains to Aztreonam, Meropenem, Cefepime, Piperacillin/Tazobactam, Gentamicin, and Imipenem were 75.6%, 73%, 94.5%, 72.9%, 81%, and 78.3%, respectively, similar to results of Shokri et al 7 study, who recorded resistance to those antibiotics were 64.5%, 85.4%, 85.4%, 74%, 79.2%, and 84.4%, respectively.…”

Section: Discussionsupporting

confidence: 91%

The Role of Lactobacillus against Pseudomonas aeruginosa infection caused by urethral catheter in Assiut Urology Hospital

Abdelmoez

2021

Egyptian Journal of Medical Microbiology

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Section: Discussionsupporting

confidence: 91%

The Role of Lactobacillus against Pseudomonas aeruginosa infection caused by urethral catheter in Assiut Urology Hospital

Abdelmoez

2021

Egyptian Journal of Medical Microbiology

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“…To concentrate the antimicrobial metabolite produced by the actinomycetes, medium were extracted using hexane (HEX), dichloromethane (DCM) and ethyl acetate (AcOEt) (1000 mL), in increasing order of polarity. Then, the organic phase, containing the active metabolite, was separated from the aqueous phase with a separatory funnel and the extracts were concentrated in a rotary evaporator at 37 °C [17]. Crude extracts obtained from the isolates were dissolved in DMSO and used as a stock solution to determine antimicrobial activity to the test pathogens, using DMSO as a control treatment.…”

Section: Secondary Screening and Molecular Identification Of Actinomy...mentioning

confidence: 99%

Antimicrobial Activity of Actinomycetes Isolated from Soils in the Brazilian Amazon

Oliveira,

Diniz,

Peters

et al. 2024

Braz. arch. biol. technol.

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The objective of this work was to evaluate the antimicrobial activity of actinomycetes isolated from soils in the Brazilian Amazon. Soil samples were collected for the isolation of actinomycetes. The actinomycetes were grown in submerged culture and the metabolites extracted in ethyl acetate. The well diffusion method was used to verify the antimicrobial activity of isolated actinomycetes against test microorganisms. The microdilution method was used to determine the minimum inhibitory concentration (MIC) of the extracts against the test microorganisms. Bioautography was used to verify the bioactive compound of the extracts. The biomass and active metabolites were determined. 219 actinomycetes were isolated from Amazonian soils, organized into 153 morphospecies. The most frequent genus were Streptomyces (48.85%) and Nocardia (20.09%). Of the 153 extracts analyzed, 12 (7.84%) had antimicrobial activity, 9 against Gram positive bacteria and 5 against Candida albicans. The MICs of the extracts with activity ranged from 15 to 250 ug/mL. The bioautography method showed that the extracts from Streptomyces 3323 HEX, Streptomyces 3098 DCM, Streptomyces 3333 DCM and Streptomyces 3333 EtOAc generally had an active substance, and that they are different from commercial antimicrobials produced by HIGHLIGHTS• The genus Streptomyces is the most frequent actinomycete in samples of only Amazonia.• The bioautography method reveal that actinomycete extracts possess an active substance against human pathogens.• Active metabolites of actinomycetes are correlated to the stationary phase of biomass production.• Actinomycetes from the Amazon show potential to produce new antibiotics.

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“…[5] Wahaab et al, 2015, have isolated marine actinomycetes from Rameswaram and have identified the strain RAM24C2 with the highest antibacterial activity against ocular pathogen Pseudomonas aeruginosa. [6] Rameswaram is one of the major hotspots for exploration of novel actinomycetes species and extraction of bioactive secondary metabolites.…”

Section: Original Articlementioning

confidence: 99%

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Каннабиран

2016

AJP

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Aim: To isolate and identify marine actinomycetes with potential antibacterial activity. Materials and Methods: Marine actinomycetes were isolated from soil samples collected at the Rameswaram and Dhanushkodi coast of Tamil Nadu, India. The isolated strains were screened for antibacterial activity by crossstreaking method against Escherichia coli and Bacillus cereus. Actinomycetes isolates demonstrated potential antibacterial activity were mass cultured and secondary metabolites were extracted using chloroform. The crude extract prepared was analyzed for antibacterial activity by well diffusion method (100 µg/0.1 ml) and also subjected to chemical characterization using gas chromatography-mass spectrometry (GC-MS) JEOL (GCMATE II GC-MS, Agilent Technologies 6890N Network GC system for GC). Results: Among the five different isolation media used, composed Starch Casein Agar (composed) proved to be most effective for isolation of actinomycetes from Rameswaram and Dhanushkodi coastal soil samples. Among the 100 isolated strains, 10 isolates (LGK001 to LGK010) demonstrated a significant antibacterial activity with the zone of inhibition ranging from 8 to 12 mm against Escherichia coli and B. cereus. Based on the GC-MS analysis, all 10 isolates produced either gancidin-W (and/or) cyclo-pro-phe as major secondary metabolites in their crude extracts. Both these molecules are previously reported antibiotic compounds. Conclusion: The results of this study suggest that Rameswaram and Dhanushkodi coastal soil samples are the rich sources for antibacterial bioactive compounds (gancidin-W and cyclo-pro-phe) producing actinomycetes.

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Comparative efficacy of macrolides containing marine actinomycetes formulation versus ciprofloxacin ophthalmic solution in controlling Pseudomonas aeruginosa induced conjunctivitis on rabbit model

Abstract: P. aeruginosa. Compared to ciprofloxacin ophthalmic solution our RAM24C2 extract formulation hastens the cure of conjunctivitis developed rabbits and need less dosage frequency.

Cited by 6 publications

References 15 publications

The Role of Lactobacillus against Pseudomonas aeruginosa infection caused by urethral catheter in Assiut Urology Hospital

The Role of Lactobacillus against Pseudomonas aeruginosa infection caused by urethral catheter in Assiut Urology Hospital

Antimicrobial Activity of Actinomycetes Isolated from Soils in the Brazilian Amazon

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