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Wound healing activity of the bark extracts of Acacia leucophloea Willd., Fabaceae, was investigated by excision and incision wound healing models in Wistar male rats. Ethanolic extract based ointment of A. leucophloea bark (2 and 5% (w/w)) was formulated and evaluated for its wound healing in Wistar male rats. In comparision with a standard wound healing ointment betadine. A. leucophloea ethanolic extract ointment exhibited marked wound healing activity and signifi cantly enhanced the wound contraction and the period of epithelialization as assessed by wound contraction rate, tensile strength, increasing of DNA, collagen and protein synthesis and histopathological examination. The formulated ointment might well fi nd use as skin repair agent without hazard to human health based on these results.