Comparative efficacy of chlorophyllin in reducing cytotoxicity of some heavy metals

Abstract

The potential of chlorophyllin in reducing clastogenicity was studied against two concentrations of each of three potent metallic clastogens (cesium chloride, mercuric chloride and cobalt chloride) in bone marrow cells of mice in vivo. The respective salts and chlorophyllin were administered orally to mice by gavaging in different combinations. Simultaneous administration of chlorophyllin with both concentrations of each salt reduced the clastogenic effects in the order Cs greater than Hg greater than Co. Chlorophyllin could not decrease the clastogenic effects when administered 2 h before the salts.

Comparison of the effects of crude extract of spinach‐beet leaves and equivalent amounts of chlorophyll and chlorophyllin in modifying the clastogenic activity of chromium (VI) oxide in mice in vivo

“…This dose has been found to be effective in reducing the clastogenicities of a number of known clastogens including chromium (VI) oxide (Ghosh et al, 1991;Sarkar et al, 1993;Sen et al, 1991).…”

“…Chlorophyllin, a sodium-copper salt of chlorophyll, obtained from Sigma (St Louis, MO, USA), was dissolved in distilled water at a dose of 1.5 mg/kg body wt (equivalent to the amount of chlorophyll extracted). This dose has been found to be effective in reducing the clastogenicities of a number of known clastogens including chromium (VI) oxide (Ghosh et al, 1991;Sarkar et al, 1993;Sen et al, 1991). Chromium (VI) oxide (CrO,, mol wt 101.01, E. Merck, India) was dissolved in distilled water and used at a dose of 20 mg/kg body wt, corresponding to a onetenth fraction of the LDx, value of this compound.…”

“…Work done in our laboratory Author to whom correspondence should be addressed. demonstrated that chlorophyllin, when given alone, is non-clastogenic and reduces to a significant extent the clastogenic activity of a number of metallic salts including chromium (VI) oxide in mammalian systems in vivo (Ghosh et al, 1991;Palit ef al., 1991;Sarkar et al, 1993;Sen et al, 1991).…”

Chemopreventive Potential of Chlorophyllin: A Review of the Mechanisms of Action and Molecular Targets

“…CHL exerted strong anticlastogenic activity against several classes of chemicals including cesium chloride, mercuric chloride, cobalt chloride, chromium (VI) oxide, N-nitroso-N-ethyl urea, and urethane in mammalian cells (16). CHL was found to reduce chromosomal aberrations induced by ethyl methane sulfonate in cultured mammalian cells during S and G2/S phase of the cell cycle as well as the formation of micronuclei (MN) and bone marrow toxicity in sodium nitrite treated mice (17,18).…”

Interactions of chlorophyllin with acridine orange, quinacrine mustard and doxorubicin analyzed by light absorption and fluorescence spectroscopy

“…It has frequently been reported during the past decade that CHL has strong anti-oxidative, antimutagenic and anti-carcinogenic properties [1]. Thus, the mutagenicity of such diverse agents as heterocyclic amines [2][3][4][5], aflatoxin B 1 [6,7], benzo[a]pyrene [8][9][10][11], dibenzo[a, 1]pyrene [12], doxorubicin [13], cyclophosphamide [14], reactive oxygen intermediates (ROIs) [15] or heavy metal ions [16][17][18], was shown to be reduced in the presence of CHL.…”