Comparative effects of perilla and fish oils on the activity and gene expression of fatty acid oxidation enzymes in rat liver

Ishii, Takashi; Kobayashi, Hideyuki; Lakshmikuttyamma, Ashakumary; Rouyer, Isabelle A.; Takahashi, Yōko; Aoyama, Toshifumi; Hashimoto, Takashi; Mizugaki, Michinao

doi:10.1016/s1388-1981(00)00026-3

Cited by 141 publications

(129 citation statements)

References 43 publications

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“…12) Enzymatic activity. The activities of hepatic enzymes (fatty acid synthase, malic enzyme, glucose-6-phosphate dehydrogenase, carnitin palmitoyltransferase and acyl CoA oxidase) were basically measured according to the methods described by Ide et al 13) Liver was homogenized with 0.25 M sucrose containing 1 mM EDTA and a 3 mM Tris-HCl buffer (pH 7.0), and then centrifuged at 500 Â g for 10 min. The supernatant fraction was centrifuged at 9000 Â g for 10 min to obtain a mitochondrial fraction.…”

Section: Methodsmentioning

confidence: 99%

Effects of Non-Methylene-Interrupted Polyunsaturated Fatty Acid, Sciadonic (All-cis-5,11,14-eicosatrienoic Acid) on Lipid Metabolism in Rats

Endo

Tsunokake

Ikeda

2009

Bioscience, Biotechnology, and Biochemistry

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We investigated effects of the non-methylene-interrupted polyunsaturated fatty acid, sciadonic acid (allcis-5,11,14-eicosatrienoic acid), on the lipid metabolism in rats, to identify the mechanism for the plasma and hepatic triacylglycerol-lowering effects of Japanese torreya (Torreya nucifera) seed oil. Sciadonic acid was isolated from torreya seed oil by the combination of urea-adduct with lipase-esterification. Sprague-Dawley (SD) male rats were fed with experimental diets containing 5% and 10% sciadonic acid based on corn oil for 2 weeks. The serum and liver triacylglycerol levels were lower in the rats fed with sciadonic acid. Considerable amounts of sciadonic acid were detected in the triacylglycerol and phospholipid in both the serum and liver of the rats fed with sciadonic acid. These observations demonstrate that sciadonic acid could modify the lipid metabolism in rats.

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Section: Methodsmentioning

confidence: 99%

Effects of Non-Methylene-Interrupted Polyunsaturated Fatty Acid, Sciadonic (All-cis-5,11,14-eicosatrienoic Acid) on Lipid Metabolism in Rats

Endo

Tsunokake

Ikeda

2009

Bioscience, Biotechnology, and Biochemistry

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“…Finally, we could also hypothesise that oxidation declined with higher doses of ALA, which would increase the pool of secreted TG and could prevent a further decrease in plasma TG concentration. This mechanism is very unlikely, since rats fed high doses of ALA (57% total fatty acids, 15-20% total energy) exhibited higher mitochondrial and peroxisomal oxidation rates than those fed palm (rich in SFA and oleic acid) or safflower (rich in LA) oil [21]. Therefore, it is possible that, in these fasting animals, triglyceridemia did not parallel the inhibition of lipogenic activity, because the decrease in de novo fatty acid synthesis is compensated for by fatty acids originating from the adipose tissue and recycled by the liver in the form of TG.…”

Section: Triglyceride Metabolismmentioning

confidence: 99%

“…Moreover, a recent epidemiological survey highlighted an inverse relation between ALA consumption and plasma TG concentration (after adjustment for other dietary parameters) [18]. Studies in the rat suggested that mechanisms involved in the decreased triglyceridemia could rely, as with n-3 LC-PUFA, on gene regulation resulting in a decreased activity [19] and expression [20] of key lipogenic enzymes and sometimes on a rise in mitochondrial oxidation [21], this latter effect being controversial [19]. Some of these discrepancies could originate from the different doses of dietary ALA used in human studies, which varied between 1.8 and 12% of total energy intake.…”

Section: Introductionmentioning

confidence: 99%

Dose effect of alpha-linolenic acid on lipid metabolism in the hamster

Morise¹,

Mourot²,

Riottot³

et al. 2005

Reprod. Nutr. Dev.

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-In order to meet dietary requirements, the consumption of α-linolenic acid (ALA, 18:3 n-3) must be promoted. However, its effects on triglyceride (TG) and cholesterol metabolism are still controversial, and may be dose-dependent. The effects of increasing dietary ALA intakes (1%, 10%, 20% and 40% of total FA) were investigated in male hamsters. ALA replaced oleic acid while linoleic and saturated FA were kept constant. Triglyceridemia decreased by 45% in response to 10% dietary ALA and was not affected by higher intakes. It was associated with lower hepatic total activities of acetyl-CoA-carboxylase (up to -29%) and malic enzyme (up to -42%), which were negatively correlated to ALA intake (r 2 = 0.33 and r 2 = 0.38, respectively). Adipose tissue lipogenesis was 2-6 fold lower than in the liver and was not affected by dietary treatment. Substitution of 10% ALA for oleic acid increased cholesterolemia by 15% but, as in TG, higher ALA intakes did not amplify the response. The highest ALA intake (40%) dramatically modified the hepatobiliary metabolism of sterols: cholesterol content fell by 45% in the liver and increased by 28% in the faeces. Besides, faecal bile acids decreased by 61%, and contained more hydrophobic and less secondary bile acids. Thus, replacing 10% oleic acid by ALA is sufficient to exert a beneficial hypotriglyceridemic effect, which may be counteracted by the slight increase in cholesterolemia. Higher intakes did not modify these parameters, but a very high dose resulted in adverse effects on sterol metabolism. α-linolenic acid / oleic acid / lipogenesis / cholesterol / triglyceride / bile acid / hamster

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“…Rat liver was homogenized in 0.2 M HEPES (pH 7.4) containing 0.1 mM EDTA and 10 mM mercaptoethanol, centrifuged at 20,000× g for 30 min at 49 C. The activities of acetyl-CoA carboxylase, FAS, malic enzyme, and glucose-6-phosphate dehydrogenase (G6PDH) were measured as detailed previously. [7][8][9][10] The activity of ATP-citrate lyase was measured according to the procedure described by Takeda et al…”

mentioning

confidence: 99%