Comparative effects of colloidal silver nanoparticles used in packaging film and spray in inactivating bacteria experimentally added to chicken eggshells

Abstract

This study focused on the inactivation of bacterial growth using a mixture containing Salmonella enteritidis, Staphylococcus aureus, Bacillus cereus, and Escherichia coli. This bacterial mixture was added to chicken eggshells and subsequently targeted for elimination using two types of silver nanoparticle (Ag-NP) delivery systems: a composite film (named cover) and a colloidal spray. Both systems were applied to the eggshells' packaging at concentrations of 500, 1000, and 2000 ppm over 28 days. A General Linear Model (GLM), repeated measurement-Analysis of Variance (ANOVA) procedure was used to analyse the effect of time and concentration of the Ag-NP materials on the inactivation of bacteria. The maximum reduction of bacterial growth resulted from the "Spray 2000 ppm", for which the value of the bacteria reached a minimum (0.93 ± 0.42) on day 7, and was calculated to be undetected on days 14 and 28, followed by Spray 1000 ppm. In other words, increasing the concentration of the Ag-NP in both coatings, either spray or cover, significantly decreased the number of bacteria colonies on the eggshells. However, the effect of packaging with different concentrations of nanocomposites and the length of the study (number of days) did not show statistically significant results.

Increasing the shelf life of pikeperch (Sander lucioperca) fillets affected by low-density polyethylene/Ag/TiO2 nanocomposites experimentally produced by sol-gel and melt-mixing methods

“…[11] Mihaly Cozmuta et al [12] studied the ability of the packaging of a silver (Ag)/ TiO 2 nanocomposite prepared by the solvent method to increase the durability of bread. Ahari et al [13] examined the microbiological properties of TiO 2 and Ag (1%, 3%, and 5%) nanocomposite coatings on dry sausages. NPs display different characteristics against microbes.…”

Influence of preparation of silver nanoparticles in carriers based on polymer/inorganic hybrids on the mineral composition of chicken eggs

“…Our assumption is consistent with the results of studies by a few authors who used exogenous processing of bird eggshells with nanosilver. So, due to the use for 28 days of colloidal spray and composite membrane which contained silver nanoparticles in concentrations of 500, 1000 and 2000 ppm, Farrokhi et al (2017) succeeded in improving the sanitary performance of edible eggshells. Treatment of hatching eggs with disinfectants based on silver nanoparticles showed significant changes in the conductivity of the eggshell of fertilized eggs and, thus, a decrease in their moisture loss, which contributed to a decrease in mortality and an increase in body weight of quails (Batkowska et al, 2017), as well as of partridges (Chmielowiec-Korzeniowska et al, 2015).…”