Comparative Effectiveness of Synthetic and Natural Antioxidants in Toxic Liver Damage by Carbon Tetrachloride

Abstract

Currently there is an active search for medicines increasing the stability of liver to pathological effects, reinforcing its neutralizing function, promoting its functional recovery after various injuries, including poisoning by industrial poisons. Therefore, the study of opportunities to reduce toxic liver damage by carbon tetrachloride introduction of synthetic and natural antioxidants is of interest. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the introduction of the succinate containing drug called Reamberin (Polysan, St.Petersburg) and licorice syrup. The animals were divided into 4 groups and each of them had 10 rats: the group with intact animals which were held in standard conditions of vivarium; the control group in which rats were given carbon tetrachloride during 3 days daily; the experimental group in which before the introduction of carbon tetrachloride animals had a daily intra-abdominal intake of the Reamberin in a dose of 100 mg/kg (20 ml/kg); the experimental group in which before the introduction of carbon tetrachloride animals had a daily oral intake of the licorice syrup in a dose of 5 ml/kg. It was found out that in the blood and in the liver of experimental animals an introduction of carbon tetrachloride during 3 days contributes to the increase of lipid hydroperoxides level (by 24-43%), of diene conjugate (by 19-47%), and of malonic dialdehyde (by 61-81%) against the decrease of antioxidant system activity in the blood of intact animals. The introduction of the succinate containing drug to rats in the conditions of oxidative stress contributes to the decrease in the blood and in the liver of lipid hydroperoxides by 13-26%, of diene conjugates by 11-28%, and of malonic dialdehyde by 30-34% in comparison with the rats of the control group. Adding of the licorice syrup to rats contributes to the reliable decrease in the blood and in the liver of lipid hydroperoxides by 6-23%, of diene conjugates by 9-24%, and of malonic dialdehyde by 25-30% in comparison with the rats of the control group. While analyzing the effect of the antioxidants on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood and in the liver of animals was reliably higher by 10-47%, of vitamin E by 13-42% in comparison with the same parameters of the rats of the control group. So, the application of the synthetic and natural antioxidants in the conditions of introduction of carbon tetrachloride of the organism of animals under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity.

Comparative effectiveness of phytoadaptogens in toxic liver damage by carbon tetrachloride

“…Результаты проведенного исследования показали (табл. 1, 2), что введение тетрахлорметана крысам сопровождается активацией процессов ПОЛ и накоплением продуктов пероксидации в крови и печени контрольных животных: увеличением содержания ГП -на 23% (кровь) и 43% (печень) в сравнении с аналогичным показателем в группе интактных крыс; ДКна 22% (кровь) и 39% (печень); МДА -на 55% (кровь) и 79% (печень), что в очередной раз подтверждает гепатотоксичность четыреххлористого углерода (CCl 4 ) и возможность использования данной модели токсического стресса в экспериментальной фармакологии [12][13][14][15][16].…”