Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Igbokwe, Abigail A.; Iyasere, Oluwaseun S.; Sobayo, R. A.; Iyasere, Seyifunmi; Animashaun, Rukayat I.; Balogun, Fatimoh A.; Aganran, Zainab O.; Fasola, M.O.; Adedokun, Afeez D.; Lakehinde, Olawale A.; Lasisi, Sodiq O.; Suleiman, Muhammad R.; Iyiola, Jamiu D.; Daramola, J. O.

doi:10.1111/rda.13393

Cited by 6 publications

(6 citation statements)

References 52 publications

(66 reference statements)

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“…Thus, the ability to protect sperm cells after the freezing process using a Tris-based extender, combined with casein at different concentrations (CAS1 = 0.125 g/l; CAS2 = 0.25 g/l; CAS3 = 0.5 g/l) is evident. The mechanism of protection of casein on gametes is not fully elucidated, but, as verified by some authors, it is believed that milk proteins protect spermatozoa through interaction with binder of sperm proteins (BSP), preventing loss of lipids through the sperm membrane, which can be harmful to sperm storage, similar to the already known protection of the Tris egg yolk-based extender (Bergeron & Manjunath, 2006;Igbokwe et al, 2019) and used in this study as a control group. However, in this study, the semen was centrifuged, removing the seminal plasma and consequently, the BSP before freezing the semen.…”

Section: Parametersmentioning

confidence: 76%

Chemically defined diluent based on Tris-casein in freezing goat semen

Souza

Monteiro²,

Seal³

et al. 2022

RSD

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The objective of the present work was to elaborate on a chemically defined extender, based on Tris-casein, and to evaluate its efficacy in the goat semen cryopreservation. For this purpose, three goats were used. Eight ejaculates were collected per male (n = 24). After semen collection, the ejaculates were frozen in a conventional extender (Tris-egg yolk) – control group, as well as an extender based on Tris-casein, in different concentrations (CAS1: 0.125 g/l; CAS2: 0.25 g/l; CAS3: 0.5 g/l), combined with 5% of glycerol. After dilution, 200 x 106 sperm/ml was frozen in an automated system and stored in liquid nitrogen (−196 ºC). After thawing (37 ºC, 30 s), the samples were submitted to kinetic analysis (CASA) and functional analysis, through plasma membrane integrity, acrosome integrity and mitochondrial membrane potential. Regarding the kinetic parameters, plasma membrane integrity, acrosome integrity and mitochondrial membrane potential, there were no significant differences (P > 0.05) between the groups evaluated, with the exception of flagellar beat cross frequency (BCF), where the CAS3 group had a higher value (P ≤ 0.05) than the control group. It can be concluded that the chemically defined extender based on Tris-casein presents a viable alternative to the conventional diluent used for freezing goat semen.

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Section: Parametersmentioning

confidence: 76%

Chemically defined diluent based on Tris-casein in freezing goat semen

Souza

Monteiro²,

Seal³

et al. 2022

RSD

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“…In contrast, significant differences in sperm quality parameters in slow versus rapid freezing have been reported. For example, there was a significant difference in sperm motility between slow and rapid freezing in Korean native bucks (Choe et al, 2006), superior post-thaw motility and cryo-survival in rapid versus slow freezing (Vutyavanich et al 2010) and improved post-thaw sperm quality parameters in slow versus rapid freezing Igbokwe et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Alternatively, plantderived products seem to overcome this problem. Post-thaw quality of goat sperm was improved when it was cryopreserved with tiger nut milk extender (Igbokwe et al, 2019). Soybean milk is rich in important components comparable to those in egg yolk, which is routinely used for protection of animal sperm from cold shock during cryopreservation (Campbell and Farrel, 2007;Zhang et al, 2009;USDA, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, soybean lecithin may provide more protection for sperm than egg yolk during the freeze-thawing process and also reduce the risk of introducing bacteria and mycoplasma into semen extenders (Fukui et al, 2008). In addition, although semen cryopreservation (mainly slow freezing and rapid freezing) enables long-term preservation of sperm (Sharma et al, 2015), there are conflicting reports on optimal cryopreservation methods (Choe et al, 2006;Vutyavanich et al, 2010;Tongdee et al, 2015;Igbokwe et al, 2019). The objective was therefore to determine effects of varying concentrations of soybean milk extender as well as comparative effects of slow and rapid freezing protocols on sperm functional, seminal oxidative stress and fertilizing end points of WAD goat sperm.…”

Section: Introductionmentioning

confidence: 99%

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Soybean milk extender improves sperm functional and oxidative stress parameters of goat sperm during slow and rapid freezing

Sorongbe

Daramola

Onagbesan

et al. 2019

Agricultura Tropica Et Subtropica

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The objective of this study was to determine effects of soybean milk extender on sperm quality end points of cryopreserved goat sperm. Pooled ejaculates from West African Dwarf (WAD) goats were diluted with various amounts (0, 5, 10, 15, 20 ml) of soybean milk (SBM) in Tris-extenders, subjected to slow or rapid freezing for 30 days and subsequently thawed and evaluated. Inclusion of SBM in extenders improved sperm quality (P < 0.05) compared to control (egg yolk-based extender) for both slow and rapid freezing methods. Semen cryopreserved with 10 or 15 % SBM extender had higher (P < 0.05) motility compared to other concentrations and the control using slow freezing. Although both cryoprotocols had higher (P < 0.05) acrosome integrity when cryopreserved with 20 % SBM extender, acrosome integrity at 20 % SBM was higher (P < 0.05) in rapid freezing compared to slow freezing. Semen cryopreserved with SBM extenders had lower (P < 0.05) Malondialdehyde (MDA) concentrations in 10 to 20 % SBM extenders for both freezing methods, although MDA concentrations were lower (P < 0.05) in slow freezing compared to rapid freezing. Semen cryopreserved with SBM extenders in both protocols had fewer (P < 0.05) leukocytes and a higher (P < 0.05) acrosome reaction and sperm capacitation, whereas there was a higher (P < 0.05) acrosome reaction with 10 and 5 % SBM extenders using slow and rapid freezing respectively. In conclusion, for goat semen cryopreserved using slow or rapid freezing protocols, SBM extenders improved functional, fertilizing and seminal oxidative stress end points.

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“…Secondly, when ice crystals form, the solute concentration and osmotic pressure in spermatozoa cells will increase, and water molecules permeate from the inside to the outside of spermatozoa cells, causing continuous contraction and dehydration of spermatozoa cells, which will lead to the damage of cell membrane structure and protein degeneration 16 , 17 . Therefore, ice crystals are the main factor causing spermatozoa death, and appropriate cooling rate can help avoid the production of ice crystals in spermatozoa as much as possible and reduce the damage to spermatozoa 18 .…”

Section: Introductionmentioning

confidence: 99%

Effect of fumigation height and time on cryopreservation of ram semen

Zhang,

Wang,

Jiang

et al. 2024

Sci Rep

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The cooling rate is a crucial factor in the process of freezing semen, influencing the overall freezing effectiveness. The height and time of fumigation can significantly impact the rate of cooling. Appropriate cooling rates can help minimize the formation of ice crystals in spermatozoa and reduce potential damage to them. Therefore, the aim of this study was to evaluate the effect of different fumigation heights and time for the cryopreservation of Hu ram semen. Experiments I–IV assessed the effect of semen cryopreservation by testing the post-thawed spermatozoa total motility (TM), progressive motility (PM) and kinetic parameters fumigated at distances of 2, 4, 6 and 8 cm for durations of 5, 10, 15 and 20 min, respectively. Based on the results of experiments I to IV, experiment V evaluated the effect of semen cryopreservation by testing the post-thawed spermatozoa TM, PM, kinetic parameters, plasma membrane integrity, acrosome integrity and reactive oxygen species (ROS) level fumigated at distances of 2, 4, 6 and 8 cm for duration of 20 min. The results indicated that fumigation at 2 cm for 20 min significantly (P < 0.05) improved spermatozoa TM, PM, mean angular displacement (MAD), plasma membrane integrity and acrosome integrity compared to other groups. Additionally, it significantly (P < 0.05) reduced spermatozoa ROS level compared to the 6 and 8 cm groups. In conclusion, fumigation for 20 min at a distance of 2 cm from the liquid nitrogen surface is the most suitable cooling method for the cryopreservation of Hu ram semen.

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Comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender

Cited by 6 publications

References 52 publications

Chemically defined diluent based on Tris-casein in freezing goat semen

Chemically defined diluent based on Tris-casein in freezing goat semen

Soybean milk extender improves sperm functional and oxidative stress parameters of goat sperm during slow and rapid freezing

Effect of fumigation height and time on cryopreservation of ram semen

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